I. Summary

SecReT6 ID	353
Strain	Pectobacterium carotovorum subsp. carotovorum PC1
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_012917
Location	3647323..3672243
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	PC1_3234	3643142..3644059 [-], 918	253689600	hypothetical protein
2	PC1_3235	3644056..3645012 [-], 957	253689601	hypothetical protein
3	PC1_3236	3645164..3645943 [-], 780	253689602	Ankyrin
4	PC1_3237	3645943..3647313 [-], 1371	253689603	PAAR repeat-containing protein	PAAR
5	PC1_3238	3647323..3649359 [-], 2037	253689604	type VI secretion system Vgr family protein	TssI
6	PC1_3239	3649589..3650107 [-], 519	253689605	Hcp1 family type VI secretion system effector	TssD
7	PC1_3240	3650646..3651998 [-], 1353	253689606	Sel1 domain-containing protein repeat-containing protein
8	PC1_3241	3652030..3653466 [-], 1437	253689607	ImpA domain-containing protein	TssA
9	PC1_3242	3653515..3657012 [-], 3498	253689608	type VI secretion protein IcmF	TssM
10	PC1_3243	3657037..3658461 [-], 1425	253689609	type VI secretion-associated protein	TssA
11	PC1_3244	3658473..3659033 [-], 561	253689610	type VI secretion-associated protein
12	PC1_3245	3659033..3660571 [-], 1539	253689611	Sigma 54 interacting domain-containing protein
13	PC1_3246	3660574..3663171 [-], 2598	253689612	type VI secretion ATPase, ClpV1 family	TssH
14	PC1_3247	3663185..3663961 [-], 777	253689613	type IV / VI secretion system protein, DotU family	TssL
15	PC1_3248	3663977..3665314 [-], 1338	253689614	type VI secretion protein	TssK
16	PC1_3249	3665317..3665838 [-], 522	253689615	type VI secretion lipoprotein, VC_A0113 family	TssJ
17	PC1_3250	3665838..3667058 [-], 1221	253689616	putative FHA domain-containing protein	Fha
18	PC1_3251	3667061..3668059 [-], 999	253689617	type VI secretion protein	TssG
19	PC1_3252	3668023..3669789 [-], 1767	253689618	type VI secretion protein	TssF
20	PC1_3253	3669792..3670223 [-], 432	253689619	type VI secretion system lysozyme-like protein	TssE
21	PC1_3254	3670229..3671707 [-], 1479	253689620	EvpB family type VI secretion protein	TssC
22	PC1_3255	3671740..3672243 [-], 504	253689621	type VI secretion protein	TssB
23	PC1_3256	3673656..3676088 [+], 2433	253689622	CRISPR-associated protein, Csm1 family
24	PC1_3257	3676113..3676496 [+], 384	253689623	CRISPR-associated protein, Csm2 family

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

Dda3937_01758 , ECA3427, ECA2866, ECA0456, ECA3427, ECA3427, ECA2866

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PC1_0051	56584..60897 [-], 4314	253686459	YD repeat-containing protein	Dda3937_01758
2	PC1_0053	61325..63178 [-], 1854	253686461	type VI secretion system Vgr family protein	ECA3427
3	PC1_0054	63269..63787 [-], 519	253686462	Hcp1 family type VI secretion system effector	ECA2866
4	PC1_0441	503878..504357 [-], 480	253686843	Hcp1 family type VI secretion system effector	ECA0456
5	PC1_2188	2518230..2520200 [-], 1971	253688573	type VI secretion system Vgr family protein	ECA3427
6	PC1_3238	3647323..3649359 [-], 2037	253689604	type VI secretion system Vgr family protein	ECA3427
7	PC1_3239	3649589..3650107 [-], 519	253689605	Hcp1 family type VI secretion system effector	ECA2866

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Proteins        Genes

N/A

N/A