I. Summary

SecReT6 ID	366
Strain	Escherichia coli O103:H2 str. 12009
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_013353
Location	3415188..3441852
Comment
Function	N/A
T6SS Type	Type i; subtype i2
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	ECO103_3353 (csdA)	3410370..3411575 [+], 1206	260845456	cysteine sulfinate desulfinase CsdA
2	ECO103_3354 (ygdK)	3411575..3412018 [+], 444	260845457	Fe-S metabolism protein
3	ECO103_3355 (ygdL)	3412069..3412875 [-], 807	260845458	hypothetical protein
4	ECO103_3356 (mltA)	3412952..3414049 [-], 1098	260845459	membrane-bound lytic murein transglycosylase A
5	ECO103_3357	3415188..3415697 [+], 510	260845460	hypothetical protein	TssB
6	ECO103_3358	3415746..3417284 [+], 1539	260845461	hypothetical protein	TssC
7	ECO103_3359	3417302..3418639 [+], 1338	260845462	hypothetical protein	TssK
8	ECO103_3360	3418636..3419301 [+], 666	260845463	hypothetical protein	TssL
9	ECO103_3361	3419314..3420966 [+], 1653	260845464	outer membrane lipoprotein	TagL
10	ECO103_3362	3421024..3421515 [+], 492	260845465	Hcp-like protein	TssD
11	ECO103_3363	3421706..3424360 [+], 2655	260845466	ATP-dependent Clp proteinase ATP-binding chain	TssH
12	ECO103_3364	3424372..3426879 [+], 2508	260845467	hypothetical protein	TssI
13	ECO103_3365	3426901..3427494 [+], 594	260845468	hypothetical protein
14	ECO103_3366	3427598..3428047 [+], 450	260845469	hypothetical protein
15	ECO103_3367	3428125..3429339 [+], 1215	260845470	hypothetical protein
16	ECO103_3368	3429396..3432722 [+], 3327	260845471	hypothetical protein	TssM
17	ECO103_3369	3432715..3434325 [+], 1611	260845472	hypothetical protein	TssA
18	ECO103_3370	3434325..3435749 [+], 1425	260845473	hypothetical protein	PAAR
19	ECO103_3371	3436724..3438484 [+], 1761	260845474	hypothetical protein	TssF
20	ECO103_3372	3438448..3439527 [+], 1080	260845475	hypothetical protein	TssG
21	ECO103_3373	3439508..3440044 [+], 537	260845476	hypothetical protein	TssJ
22	ECO103_3374	3440048..3440476 [+], 429	260845477	hypothetical protein	TssE
23	ECO103_3375	3440476..3441852 [+], 1377	260845478	hypothetical protein	TssA
24	ECO103_3376 (amiC)	3442811..3444064 [-], 1254	260845479	N-acetylmuramoyl-L-alanine amidase
25	ECO103_3377 (argA)	3444296..3445627 [+], 1332	260845480	fused acetylglutamate kinase homolog (inactive)/amino acid N-acetyltransferase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

EC042_4529

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	ECO103_3362	3421024..3421515 [+], 492	260845465	Hcp-like protein	EC042_4529

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Proteins        Genes

N/A

N/A