I. Summary
SecReT6 ID37 experimental
StrainEscherichia coli CFT073
Repliconchromosome [Browse all T6SS(s) in this replicon]
CommentPAI-metV2 genomic island mutant (c3398-c3410 deleted) was severely attenuated in its ability to colonize the bladders of mice.
Functionanti-eukaryotic; virulence
T6SS TypeType i; subtype i2
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1C_RS16000 (fucU)3213754..3214176 [+], 423446843584L-fucose mutarotase 
2C_RS16005 (fucR)3214234..3214965 [+], 732485663863L-fucose operon activator 
3C_RS16010 (rlmM)3215009..3216109 [-], 110144696827423S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM 
4C_RS160153216102..3216497 [-], 396446126055DUF423 domain-containing protein 
5C_RS16020 (gcvA)3216516..3217433 [-], 918445966546glycine cleavage system transcriptional regulator GcvA 
6C_RS160253217784..3218011 [-], 228446673052YgdI/YgdR family lipoprotein 
7C_RS16030 (csdA)3218203..3219408 [+], 1206485693493cysteine desulfurase CsdA 
8C_RS16035 (csdE)3219408..3219851 [+], 444446106417cysteine desulfurase sulfur acceptor subunit CsdE 
9C_RS16040 (tcdA)3219902..3220708 [-], 807446039856tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA 
10C_RS16045 (mltA)3220785..3221882 [-], 1098446601304murein transglycosylase A 
11C_RS16065 (tssB)3223023..3223523 [+], 501485828321type VI secretion system contractile sheath small subunit 
12C_RS16070 (tssC)3223582..3225120 [+], 1539485718542type VI secretion system contractile sheath large subunit 
13C_RS16075 (tssK)3225140..3226477 [+], 1338446609343type VI secretion system baseplate subunit TssK 
14C_RS160803226474..3227139 [+], 666446634969type VI secretion system protein TssL, short form 
15C_RS160853227152..3228804 [+], 1653447169270OmpA family protein 
16C_RS160903228862..3229353 [+], 492446919812Hcp family type VI secretion system effector  TssD
17C_RS16095 (tssH)3229545..3232181 [+], 2637446069484type VI secretion system ATPase TssH 
18C_RS16100 (vgrG)3232193..3234655 [+], 2463486165960type VI secretion system tip protein VgrG 
19C_RS161053234652..3235494 [+], 843485663865hypothetical protein 
20C_RS161103235491..3237467 [+], 1977446028334hypothetical protein 
21C_RS161153237489..3237749 [+], 261485828323PAAR domain-containing protein 
22C_RS161203237749..3239182 [+], 1434446462015hypothetical protein 
23C_RS161253239189..3242527 [+], 3339446755471type VI secretion protein VasK 
24C_RS16130 (tssA)3242520..3244130 [+], 1611485828325type VI secretion system protein TssA 
25C_RS16135 (tssF)3244164..3245922 [+], 1759pseudogenetype VI secretion system baseplate subunit TssF 
26C_RS16140 (tssG)3245886..3246965 [+], 1080446295459type VI secretion system baseplate subunit TssG 
27C_RS16145 (tssJ)3246946..3247482 [+], 537446406153type VI secretion system lipoprotein TssJ 
28C_RS16150 (tssE)3247486..3247914 [+], 429446029112type VI secretion system baseplate subunit TssE 
29C_RS161553247914..3249290 [+], 1377446079962type VI secretion system ImpA family N-terminal domain-containing protein 
30C_RS161603249590..3250537 [-], 948485663869phosphoglycerate dehydrogenase 
31C_RS161653250609..3251205 [-], 597446988967SIS domain-containing protein 
32C_RS161703251208..3252383 [-], 1176446304558PatB family C-S lyase 
33C_RS161753252383..3253963 [-], 1581446733301PTS transporter subunit EIIC 
34C_RS161803253995..3254819 [-], 825486165963PRD domain-containing protein 
35C_RS16185 (amiC)3255077..3256330 [-], 1254445939052N-acetylmuramoyl-L-alanine amidase AmiC 
36C_RS16190 (argA)3256562..3257893 [+], 1332446160092amino-acid N-acetyltransferase 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

Download FASTA format files
Proteins        Genes
(1) Lloyd AL et al. (2009). Genomic islands of uropathogenic Escherichia coli contribute to virulence. J Bacteriol. 191(11):3469-81. [PudMed:19329634] experimental
(2) Boyer F et al. (2009). Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 10:104. [PudMed:19284603] in_silico
(3) Leiman PG et al. (2009). Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc Natl Acad Sci U S A. 106(11):4154-9. [PudMed:19251641]
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation