I. Summary
SecReT6 ID37 experimental
StrainEscherichia coli CFT073
Repliconchromosome [Browse all T6SS(s) in this replicon]
CommentPAI-metV2 genomic island mutant (c3398-c3410 deleted) was severely attenuated in its ability to colonize the bladders of mice.
Functionanti-eukaryotic; virulence
T6SS TypeType i; subtype i2
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1c3374 (fucU)3213754..3214176 [+], 42326249209fucose operon fucU protein 
2c3375 (fucR)3214225..3214965 [+], 74126249210DNA-binding transcriptional activator FucR 
3c3376 (ygdE)3215009..3216109 [-], 110126249211RNA 2'-O-ribose methyltransferase 
4c3377 (ygdD)3216102..3216404 [-], 30326249212hypothetical protein 
5c3378 (gcvA)3216516..3217433 [-], 91826249213DNA-binding transcriptional activator GcvA 
6c33793217751..3218044 [+], 29426249214hypothetical protein 
7c3380 (ygdI)3217784..3218014 [-], 23126249215lipoprotein ygdI 
8c33813218167..3219408 [+], 124226249216cysteine sulfinate desulfinase 
9c3382 (ygdK)3219408..3219851 [+], 44426249217hypothetical protein 
10c3383 (ygdL)3219902..3220708 [-], 80726249218hypothetical protein 
11c3384 (mltA)3220785..3221882 [-], 1098161486137murein transglycosylase A 
12c33853223002..3223523 [+], 52226249220hypothetical protein  TssB
13c33863223576..3225120 [+], 154526249221hypothetical protein  TssC
14c33873225140..3226477 [+], 133826249222hypothetical protein  TssK
15c33883226474..3227139 [+], 66626249223hypothetical protein  TssL
16c33893227152..3228804 [+], 165326249224hypothetical protein  TagL
17c33903228841..3229443 [-], 60326249225hypothetical protein 
18c33913228862..3229353 [+], 49226249226secreted protein Hcp  TssD
19c33923229545..3232181 [+], 263726249227ClpB protein  TssH
20c33933232181..3234655 [+], 247526249228hypothetical protein  TssI
21c33943234670..3235494 [+], 82526249229hypothetical protein 
22c33953235491..3237467 [+], 197726249230hypothetical protein 
23c33963237477..3237749 [+], 27326249231hypothetical protein  PAAR
24c33973237749..3239182 [+], 143426249232hypothetical protein 
25c33983239189..3242527 [+], 333926249233hypothetical protein  TssM
26c33993242493..3244130 [+], 163826249234hypothetical protein  TssA
27c34003244161..3246965 [+], 280526249235hypothetical protein  TssF
28c34013246946..3247482 [+], 53726249236hypothetical protein  TssJ
29c34023247486..3247914 [+], 42926249237hypothetical protein  TssE
30c34033247914..3249290 [+], 137726249238hypothetical protein  TssA
31c34043248464..3248616 [-], 15326249239hypothetical protein 
32c34053249590..3250546 [-], 957262492402-hydroxyacid dehydrogenase 
33c34063250609..3251205 [-], 59726249241phosphosugar isomerase 
34c34073251208..3252383 [-], 117626249242Beta-cystathionase 
35c34083252383..3253963 [-], 158126249243PTS system, maltose and glucose-specific IIABC component 
36c34093253995..3254876 [-], 88226249244antiterminator 
37c34103254915..3255067 [+], 15326249245hypothetical protein 
38c34113255077..3256420 [-], 134426249246N-acetylmuramoyl-L-alanine amidase 
39c3412 (argA)3256562..3257893 [+], 1332161486136N-acetylglutamate synthase 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1c33913228862..3229353 [+], 49226249226secreted protein Hcp EC042_4529

Download FASTA format files
Proteins        Genes
(1) Lloyd AL et al. (2009). Genomic islands of uropathogenic Escherichia coli contribute to virulence. J Bacteriol. 191(11):3469-81. [PudMed:19329634] experimental
(2) Boyer F et al. (2009). Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 10:104. [PudMed:19284603] in_silico
(3) Leiman PG et al. (2009). Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc Natl Acad Sci U S A. 106(11):4154-9. [PudMed:19251641]
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation