I. Summary
SecReT6 ID395 experimental
StrainEnterobacter cloacae subsp. cloacae ATCC 13047
Repliconchromosome [Browse all T6SS(s) in this replicon]
CommentRhsA and RhsB confer significant T6S-dependent interbacterial fitness in donor strains grown in competition with recipient strains lacking cognate immunity genes
Functioneffector translocation
T6SS TypeType i; subtype i3
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1ECL_015291547578..1548345 [+], 768296101891putative ABC phosphate/phosphonate transporter, periplasmic ligand binding protein 
2ECL_015301548311..1550599 [-], 2289296101892hypothetical protein 
3ECL_015311550726..1550851 [+], 126296101893hypothetical protein 
4ECL_015321551462..1551956 [+], 495296101894hypothetical protein 
5ECL_01533 (vasD)1551980..1552483 [+], 504296101895type VI secretion system protein VasD  TssJ
6ECL_01534 (vasE)1552509..1553852 [+], 1344296101896type VI secretion system protein ImpJ  TssK
7ECL_01535 (impK)1553870..1555108 [+], 1239296101897type VI secretion system protein ImpK  TssL
8ECL_01536 (impL)1555111..1558731 [+], 3621296101898type VI secretion system protein ImpL  TssM
9ECL_01537 (impM)1558748..1559464 [+], 717296101899type VI secretion system protein ImpM 
10ECL_01538 (impA)1559476..1560492 [+], 1017296101900type VI secretion system protein ImpA  TssA
11ECL_01539 (impB)1560568..1561092 [+], 525296101901type VI secretion system protein ImpB  TssB
12ECL_01540 (impC)1561096..1562595 [+], 1500296101902type VI secretion system protein ImpC  TssC
13ECL_01541 (hcp)1562931..1563413 [+], 483296101903type VI secretion system secreted protein Hcp  TssD
14ECL_015421563472..1563963 [+], 492296101904putative cytoplasmic protein  ECL_01542 (e)
15ECL_015431563960..1564313 [+], 354296101905hypothetical protein  ECL_01543 (i)
16ECL_015441564401..1566170 [+], 1770296101906FHA domain-containing protein  Fha
17ECL_015451566167..1566964 [+], 798296101907protein serine/threonine phosphatase 
18ECL_015461566981..1567967 [+], 987296101908hypothetical protein 
19ECL_01547 (impE)1567977..1568789 [+], 813296101909type VI secretion system protein ImpE  TagJ
20ECL_01548 (impF)1568782..1569345 [+], 564296101910type VI secretion system protein ImpF  TssE
21ECL_01549 (impG)1569342..1571219 [+], 1878296101911type VI secretion system protein ImpG  TssF
22ECL_01550 (impH)1571216..1572259 [+], 1044296101912type VI secretion system protein ImpH  TssG
23ECL_01551 (vasG)1572298..1574913 [+], 2616296101913type VI secretion system protein VasG  TssH
24ECL_015521574938..1576371 [+], 1434296101914hypothetical protein 
25ECL_015531576408..1577826 [+], 1419296101915hypothetical protein 
26ECL_015541577823..1578584 [+], 762296101916hypothetical protein 
27ECL_015551579446..1580414 [+], 969296101917putative IS903 transposase 
28ECL_015561580667..1581512 [+], 846296101918hypothetical protein 
29ECL_015571581563..1581910 [+], 348296101919hypothetical protein 
30ECL_015581582007..1583935 [+], 1929296101920hypothetical protein  TssI
31ECL_015591584430..1585398 [+], 969296101921putative IS903 transposase 
32ECL_015601585771..1586151 [+], 381296101922ADP-ribosylglycohydrolase family protein 
33ECL_015611586973..1588583 [+], 1611296101923hypothetical protein  TssI
34ECL_015621588556..1588894 [+], 339296101924hypothetical protein 
35ECL_015631588905..1589387 [+], 483296101925hypothetical protein 
36ECL_015641589389..1589943 [+], 555296101926hypothetical protein 
37ECL_015651589947..1591023 [+], 1077296101927hypothetical protein 
38ECL_015661591162..1591539 [+], 378296101928hypothetical protein 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
EC042_4529 , SMA2263, ECL_01542, SMA2276, ECL_01567 (RhsA), ECL_03140 (RhsB)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1ECL_013771397170..1397652 [-], 483296101741Hcp family type VI secretion system effector EC042_4529
2ECL_01541(hcp)1562931..1563413 [+], 483296101903type VI secretion system secreted protein Hcp SMA2263
3ECL_015421563472..1563963 [+], 492296101904putative cytoplasmic protein ECL_01542
4ECL_015611586973..1588583 [+], 1611296101923hypothetical protein SMA2276
5ECL_015671591543..1595931 [+], 4389296101929hypothetical protein (RhsA)ECL_01567 (RhsA)
6ECL_031403200343..3204599 [-], 4257296103479Rhs family protein (RhsB)ECL_03140 (RhsB)
Data from experimental literature

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Proteins        Genes

Homology Immunity protein list
ECL_01543, ECL_01568 (RhsIA), ECL_03139 (RhsIB)

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1ECL_015431563960..1564313 [+], 354296101905hypothetical protein ECL_01543
2ECL_015681595931..1596929 [+], 999296101930hypothetical protein (RhsIA)ECL_01568 (RhsIA)
3ECL_031393199746..3200309 [-], 564296103478hypothetical protein (RhsIB)ECL_03139 (RhsIB)
Data from experimental literature

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Proteins        Genes
(1) Whitney JC et al. (2014). Genetically distinct pathways guide effector export through the type VI secretion system. Mol Microbiol. 92(3):529-42. [PudMed:24589350] experimental
(2) Zhang H et al. (2013). Insights into the Cross-Immunity Mechanism within Effector Families of Bacteria Type VI Secretion System from the Structure of StTae4-EcTai4 Complex. PLoS One. 8(9):e73782. [PudMed:24023903] experimental in_silico
(3) Zhang H et al. (2013). Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein. J Biol Chem. 288(8):5928-39. [PudMed:23288853] experimental in_silico
(4) Russell AB et al. (2012). A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806] experimental in_silico
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation