I. Summary

SecReT6 ID	399
Strain	Xenorhabdus nematophila ATCC 19061
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_014228
Location	4336814..4358257
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	0	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

13

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	XNC1_4528	4332359..4332532 [+], 174	300725281	hypothetical protein
2	XNC1_4529	4332650..4333240 [+], 591	300725282	hypothetical protein
3	XNC1_4530	4333723..4333974 [-], 252	300725283	hypothetical protein
4	XNC1_4531	4333967..4334377 [-], 411	300725284	transposase
5	XNC1_4532	4334475..4334798 [-], 324	300725285	transposase
6	XNC1_4533	4334715..4335029 [+], 315	300725286	hypothetical protein
7	XNC1_4534	4335016..4335510 [+], 495	300725287	hypothetical protein
8	XNC1_4535	4335492..4336364 [+], 873	300725288	transposase
9	XNC1_4536	4336301..4336441 [+], 141	300725289	transposase
10	XNC1_4537	4336570..4336713 [+], 144	300725290	transposase (fragment)
11	XNC1_4538	4336814..4338214 [-], 1401	300725291	hypothetical protein	TssA
12	XNC1_4539	4338297..4341902 [-], 3606	300725292	hypothetical protein	TssM
13	XNC1_4540	4341899..4343341 [-], 1443	300725293	hypothetical protein	TssA
14	XNC1_4541	4343347..4344018 [-], 672	300725294	hypothetical protein
15	XNC1_4542	4344015..4344812 [-], 798	300725295	hypothetical protein
16	XNC1_4544	4344812..4347547 [-], 2736	300725296	ClpA/B-type chaperone	TssH
17	XNC1_4545	4347557..4348324 [-], 768	300725297	hypothetical protein	TssL
18	XNC1_4546	4348327..4349679 [-], 1353	300725298	hypothetical protein	TssK
19	XNC1_4547	4349682..4350236 [-], 555	300725299	hypothetical protein	TssJ
20	XNC1_4548	4350220..4351515 [-], 1296	300725300	hypothetical protein	Fha
21	XNC1_4549	4351521..4352573 [-], 1053	300725301	hypothetical protein	TssG
22	XNC1_4550	4352537..4354369 [-], 1833	300725302	hypothetical protein	TssF
23	XNC1_4551	4354370..4354810 [-], 441	300725303	hypothetical protein	TssE
24	XNC1_4552	4354813..4356291 [-], 1479	300725304	hypothetical protein	TssC
25	XNC1_4553	4356311..4356808 [-], 498	300725305	hypothetical protein	TssB
26	XNC1_4554 (hcp)	4357739..4358257 [+], 519	300725306	hemolysin-coregulated protein Hcp	TssD
27	XNC1_4555 (xhlB)	4358887..4360560 [+], 1674	300725307	XhlB, XhlA hemolysin secretion/activation protein (TpsB)

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

ECA4275

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	XNC1_4554(hcp)	4357739..4358257 [+], 519	300725306	hemolysin-coregulated protein Hcp	ECA4275

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Proteins        Genes

N/A

N/A