I. Summary
SecReT6 ID4 experimental
StrainPseudomonas aeruginosa PAO1
Repliconchromosome (H2-T6SS) [Browse all T6SS(s) in this replicon]
CommentH2-T6SS modulates internalization in epithelial cells; virulence in the worm model; virulence (slow killing) in Caenorhabditis elegans model; PldA achieves its antibacterial activity by degrading phosphatidylethanolamine; PldA contributes to the internalization of P. aeruginosa into Human Epithelial Cells and activates the PI3K/Akt Pathway during P. aeruginosa Infection.
Functionanti-eukaryotic and anti-bacterial; effector translocation;virulence
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1PA16471793019..1794740 [-], 172215596844sulfate transporter 
2PA16481794883..1795887 [-], 100515596845oxidoreductase 
3PA16491796138..1796899 [+], 76215596846short-chain dehydrogenase 
4PA16501797058..1797951 [+], 89415596847transporter 
5PA16511797958..1799154 [-], 119715596848transporter 
6PA16521799385..1800020 [+], 63615596849hypothetical protein 
7PA16531800071..1800547 [-], 47715596850transcriptional regulator 
8PA16541800629..1801795 [+], 116715596851aminotransferase 
9PA16551801851..1802453 [+], 60315596852glutathione S-transferase 
10PA16561803626..1805182 [+], 155715596853hypothetical protein  TssA
11PA16571805218..1805724 [+], 50715596854hypothetical protein  TssB
12PA16581805753..1807228 [+], 147615596855hypothetical protein  TssC
13PA16591807241..1807648 [+], 40815596856hypothetical protein  TssE
14PA16601808193..1809773 [+], 158115596857hypothetical protein  TssF
15PA16611809737..1810744 [+], 100815596858hypothetical protein  TssG
16PA16621810751..1813384 [+], 263415596859ClpA/B-type protease  TssH
17PA16631813395..1814906 [+], 151215596860transcriptional regulator  Sfa2
18PA16641814995..1815135 [+], 14115596861hypothetical protein 
19PA16651815153..1816346 [+], 119415596862hypothetical protein  Fha
20PA16661816352..1816858 [+], 50715596863hypothetical protein  TssJ
21PA16671816855..1818186 [+], 133215596864hypothetical protein  TssK
22PA16681818189..1819058 [+], 87015596865hypothetical protein  TssL
23PA16691819074..1822601 [+], 352815596866hypothetical protein  TssM
24PA1670 (stp1)1822601..1823329 [+], 72915596867serine/threonine phosphoprotein phosphatase Stp1  Stp1
25PA1671 (stk1)1823326..1824315 [+], 99015596868serine-threonine kinase Stk1  Stk1
26PA16721824343..1824723 [-], 38115596869hypothetical protein 
27PA16731824969..1825430 [+], 46215596870bacteriohemerythrin 
28PA1674 (folE2)1825495..1826040 [-], 54615596871GTP cyclohydrolase I 
29PA16751826118..1826675 [-], 55815596872hypothetical protein 
30PA16761826732..1827052 [-], 32115596873hypothetical protein 
31PA16771827225..1827821 [-], 59715596874hypothetical protein 
32PA16781827992..1828906 [+], 9151559687550S ribosomal protein L3 glutamine methyltransferase 
33PA16791828931..1829710 [-], 78015596876hypothetical protein 
34PA16801829788..1830771 [-], 98415596877hypothetical protein 
35PA1681 (aroC)1830879..1831970 [+], 109215596878chorismate synthase 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
PA0260 , PSPTO_5435, PA1510, PSPTO_5435, PA3487 (Tle5), PSPTO_5435

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA0260291154..293304 [-], 215115595457hypothetical protein PA0260
2PA0263(hcpC)297043..297561 [-], 51915595460secreted protein Hcp PSPTO_5435
3PA15101639791..1641500 [-], 171015596707hypothetical protein PA1510
4PA1512(hcpA)1644207..1644725 [-], 51915596709secreted protein Hcp PSPTO_5435
5PA3487(pldA)3902663..3905962 [+], 330015598683phospholipase D (Tle5)PA3487 (Tle5)
6PA5267(hcpB)5932877..5933395 [-], 51915600460secreted protein Hcp PSPTO_5435
Data from experimental literature

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Proteins        Genes

Homology Immunity protein list
PA0261, PA1509, PA3488 (Tli5)

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA0261293301..293798 [-], 49815595458hypothetical protein PA0261
Data from experimental literature

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Proteins        Genes
Regulatory element identified
LasR (PA1430)ActivatorPMID: 19497948

MvfR (PA1003)ActivatorPMID: 15686549

PsrA (PA3006)RepressorPMID: 18524913

Fur (PA4764)RepressorPMID: 22665491

RpoN Sigma 54 factor (PA4462)RepressorPMID: 24204589

MvaT (PA4315)RepressorPMID: 19028873

sfa2 (PA1663)Repressor: rpoNPMID: 24204589

RhlI (PA3476)Indirect activatorPMID: 22665491
RhlR (PA3477)Indirect activatorPMID: 22665491

AmrZ (PA3385)RepressorPMID: 24603766

Adaptors list

Adaptor identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA0259289562..291004 [-], 144315595456hypothetical protein PA0259
Data from experimental literature

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Proteins        Genes
The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1PA1244 (QslA)QS regulator-H2-T6SS (PA1656) promotorThe expression of H2-T6SS machinery genes was controlled at a transcriptional level by QslA.
2PA4778 (CueR)QS-H2-T6SS.
3PA4778 (CueR)hcp2 (-208 to -159 relative to the ATG start codon).
4PA4778 (CueR)Transcription regulator+tssA2 (-173 to -140 relative to the ATG start codon)t CueR only impacts H2-T6SS activity, through binding the sequence of tssA2 and hcp2, but not the H1- and H3-T6SSs.
(1) Sana TG et al. (2015). Internalization of Pseudomonas aeruginosa Strain PAO1 into Epithelial Cells Is Promoted by Interaction of a T6SS Effector with the Microtubule Network. MBio. 6(3):e00712. [PudMed:26037124] experimental
(2) Jiang F et al. (2014). A Pseudomonas aeruginosa Type VI Secretion Phospholipase D Effector Targets Both Prokaryotic and Eukaryotic Cells. Cell Host Microbe. 15(5):600-10. [PudMed:24832454] experimental
(3) Sana TG et al. (2013). Divergent Control of Two Type VI Secretion Systems by RpoN in Pseudomonas aeruginosa. PLoS One. 8(10):e76030. [PudMed:24204589] experimental
(4) Russell AB et al. (2013). Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 496(7446):508-12. [PudMed:23552891] experimental
(5) Sana TG et al. (2012). The Second Type VI Secretion System of Pseudomonas aeruginosa Strain PAO1 Is Regulated by Quorum Sensing and Fur and Modulates Internalization in Epithelial Cells. J Biol Chem. 287(32):27095-105. [PudMed:22665491] experimental
(6) Osipiuk J et al. (2011). Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa. J Struct Funct Genomics. 12(1):21-6. [PudMed:21476004]
(7) Barret M et al. (2011). Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] in_silico
(8) Hachani A et al. (2011). Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 286(14):12317-27. [PudMed:21325275] experimental
(9) Bleves S et al. (2010). Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol. 300(8):534-43. [PudMed:20947426]
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation