I. Summary

SecReT6 ID	400
Strain	Acinetobacter oleivorans DR1
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_014259
Location	2617926..2655914
Comment
Function	N/A
T6SS Type	Type i; subtype i4b
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	AOLE_12165	2612981..2614162 [-], 1182	299770667	cyanate permease
2	AOLE_12170	2614162..2614644 [-], 483	299770668	guanine deaminase
3	AOLE_12175	2614661..2615329 [-], 669	299770669	ankyrin repeat-containing protein
4	AOLE_12180	2615478..2616335 [+], 858	299770670	LysR family transcriptional regulator
5	AOLE_12185	2616332..2617282 [-], 951	299770671	D-alanyl-D-alanine carboxypeptidase
6	AOLE_12190	2617293..2617913 [-], 621	299770672	hypothetical protein
7	AOLE_12195	2617926..2618732 [-], 807	299770673	DotU family type IV / VI secretion system protein	TssL
8	AOLE_12200	2618750..2620114 [-], 1365	299770674	type VI secretion protein	TssK
9	AOLE_12205	2620131..2621225 [-], 1095	299770675	ImpA-related N-terminal family protein	TssA
10	AOLE_12210	2621247..2623925 [-], 2679	299770676	chaperone ATPase	TssH
11	AOLE_12215	2624076..2624339 [-], 264	299770677	hypothetical protein	PAAR
12	AOLE_12220	2624356..2625123 [-], 768	299770678	OmpA/MotB domain-containing protein	TagL
13	AOLE_12225	2625126..2626085 [-], 960	299770679	hypothetical protein
14	AOLE_12230	2626123..2629944 [-], 3822	299770680	type VI secretion protein IcmF	TssM
15	AOLE_12235	2630169..2630300 [+], 132	299770681	hypothetical protein
16	AOLE_12240	2631381..2632379 [-], 999	299770682	hypothetical protein	TssG
17	AOLE_12245	2632343..2634154 [-], 1812	299770683	type VI secretion protein	TssF
18	AOLE_12250	2634171..2634647 [-], 477	299770684	type VI secretion system lysozyme-like protein	TssE
19	AOLE_12255	2634726..2635229 [-], 504	299770685	type VI secretion system effector	TssD
20	AOLE_12260	2635279..2636760 [-], 1482	299770686	type VI secretion protein EvpB	TssC
21	AOLE_12265	2636753..2637256 [-], 504	299770687	type VI secretion protein	TssB
22	AOLE_12270	2637273..2637962 [-], 690	299770688	hypothetical protein
23	AOLE_12275	2638655..2639560 [-], 906	299770689	hypothetical protein
24	AOLE_12280	2639577..2640347 [-], 771	299770690	hypothetical protein
25	AOLE_12285	2640399..2641142 [-], 744	299770691	hypothetical protein
26	AOLE_12290	2641144..2643897 [-], 2754	299770692	hypothetical protein
27	AOLE_12295	2643890..2644282 [-], 393	299770693	hypothetical protein
28	AOLE_12300	2644279..2644809 [-], 531	299770694	hypothetical protein
29	AOLE_12305	2644811..2647438 [-], 2628	299770695	hypothetical protein	TssI
30	AOLE_12310	2647622..2648122 [-], 501	299770696	hypothetical protein
31	AOLE_12315	2648211..2648747 [-], 537	299770697	hypothetical protein
32	AOLE_12320	2648825..2649343 [-], 519	299770698	hypothetical protein
33	AOLE_12325	2649361..2650224 [-], 864	299770699	hypothetical protein
34	AOLE_12330	2650619..2651602 [-], 984	299770700	hypothetical protein
35	AOLE_12335	2651617..2653017 [-], 1401	299770701	hypothetical protein
36	AOLE_12340	2653017..2655914 [-], 2898	299770702	hypothetical protein	TssI
37	AOLE_12345	2656021..2656917 [-], 897	299770703	LysR family transcriptional regulator
38	AOLE_12350	2657016..2658356 [+], 1341	299770704	MFS-family transporter
39	AOLE_12355	2658349..2658750 [+], 402	299770705	hypothetical protein
40	AOLE_12360	2658846..2659790 [+], 945	299770706	Taurine transport system permease protein tauC
41	AOLE_12365	2659790..2660671 [+], 882	299770707	ABC transporter ATP-binding protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

A1S_1296

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	AOLE_12255	2634726..2635229 [-], 504	299770685	type VI secretion system effector	A1S_1296

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Proteins        Genes

N/A

N/A