I. Summary

SecReT6 ID	404
Strain	Ralstonia solanacearum PSI07
Replicon	megaplasmid [Browse all T6SS(s) in this replicon]
Accession	NC_014310
Location	798603..845489
Comment
Function	N/A
T6SS Type	Type i; subtype i4b
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	3	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	RPSI07_mp0647	794068..794910 [+], 843	300693657	amino-acid-binding periplasmic ABC transporter protein
2	RPSI07_mp0648	795142..796086 [-], 945	300693658	hypothetical protein
3	RPSI07_mp0649	796108..796893 [-], 786	300693659	hypothetical protein
4	RPSI07_mp0650	797066..798094 [-], 1029	300693660	hypothetical protein
5	RPSI07_mp0651	798603..801947 [-], 3345	300693661	rhs element vgr-like protein	TssI
6	RPSI07_mp0652	802006..802797 [-], 792	300693662	secretion system protein	TssL
7	RPSI07_mp0653	802794..804140 [-], 1347	300693663	hypothetical protein	TssK
8	RPSI07_mp0654	804196..804777 [-], 582	300693664	hypothetical protein	TssJ
9	RPSI07_mp0655	805150..805806 [+], 657	300693665	hypothetical protein
10	RPSI07_mp0656	805843..806355 [+], 513	300693666	hypothetical protein	TssB
11	RPSI07_mp0657	806348..807838 [+], 1491	300693667	hypothetical protein	TssC
12	RPSI07_mp0658 (sciK)	807927..808430 [+], 504	300693668	secretion system protein (evpc-like), hemolysin-coregulated protein	TssD
13	RPSI07_mp0659	808491..808964 [+], 474	300693669	secretion system protein	TssE
14	RPSI07_mp0660	809027..810877 [+], 1851	300693670	type VI secretion protein (duf879)	TssF
15	RPSI07_mp0661	810841..811944 [+], 1104	300693671	type VI secretion protein, vc_a0111 family	TssG
16	RPSI07_mp0662 (clpB)	811977..814694 [+], 2718	300693672	chaperone clpb	TssH
17	RPSI07_mp0663	814715..817465 [+], 2751	300693673	rhs element vgr-related protein	TssI
18	RPSI07_mp0665	818443..821793 [+], 3351	300693674	hypothetical protein
19	RPSI07_mp0667	822743..823744 [+], 1002	300693675	hypothetical protein
20	RPSI07_mp0668	823767..824930 [-], 1164	300693676	transposase
21	RPSI07_mp0669	824996..825367 [-], 372	300693677	transposase, is4-like
22	RPSI07_mp0671	825865..826932 [+], 1068	300693678	hypothetical protein
23	RPSI07_mp0672	826941..828038 [+], 1098	300693679	hypothetical protein
24	RPSI07_mp0673	828373..829503 [+], 1131	300693680	hypothetical protein
25	RPSI07_mp0674	829487..830860 [+], 1374	300693681	rhs element vgr-related protein (fragment)	TssI
26	RPSI07_mp0675	830839..830964 [+], 126	300693682	hypothetical protein
27	RPSI07_mp0676	831006..831332 [-], 327	300693683	hypothetical protein
28	RPSI07_mp0677	831373..831786 [+], 414	300693684	hypothetical protein
29	RPSI07_mp0678	831915..833258 [+], 1344	300693685	lipoprotein yddw
30	RPSI07_mp0679	833457..834467 [+], 1011	300693686	hypothetical protein	TssA
31	RPSI07_mp0680	834653..835105 [-], 453	300693687	hypothetical protein
32	RPSI07_mp0681	835136..836020 [-], 885	300693688	hypothetical protein
33	RPSI07_mp0682	836020..836592 [-], 573	300693689	hypothetical protein
34	RPSI07_mp0683	836592..838163 [-], 1572	300693690	hypothetical protein
35	RPSI07_mp0684	838218..839090 [-], 873	300693691	hypothetical protein
36	RPSI07_mp0685	839738..840580 [-], 843	300693692	outer membrane protein; ompa/motb domain	TagL
37	RPSI07_mp0686	840577..841563 [-], 987	300693693	hypothetical protein
38	RPSI07_mp0687	841560..845489 [-], 3930	300693694	secretion system protein	TssM
39	RPSI07_mp0688	845527..846984 [-], 1458	300693695	hypothetical protein
40	RPSI07_mp0689	847025..847294 [-], 270	300693696	hypothetical protein	PAAR
41	RPSI07_mp0690	847334..848146 [-], 813	300693697	carboxypeptidase
42	RPSI07_mp0691	848468..849259 [+], 792	300693698	transmembrane protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

BCAL0343

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	RPSI07_mp0658(sciK)	807927..808430 [+], 504	300693668	secretion system protein (evpc-like), hemolysin-coregulated protein	BCAL0343

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Proteins        Genes

N/A

N/A