I. Summary
SecReT6 ID44 experimental
StrainVibrio parahaemolyticus RIMD 2210633
Repliconchromosome II [Browse all T6SS(s) in this replicon]
CommentAdhesion to cultured cell monolayers
Functionanti-eukaryotic;effector translocation;adherence
T6SS TypeType i; subtype i5
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1VPA10161069624..1070757 [-], 113428900871multidrug resistance protein D 
2VPA10171070897..1071676 [+], 78028900872AraC family transcriptional regulator 
3VPA10181071695..1072216 [-], 52228900873lipoprotein Blc 
4VPA10191072272..1072799 [-], 52828900874hypothetical protein 
5VPA10201072830..1073354 [-], 52528900875hypothetical protein 
6VPA10211073754..1074143 [+], 39028900876hypothetical protein 
7VPA10221074208..1077264 [-], 305728900877exonuclease SbcC 
8VPA10231077274..1078407 [-], 113428900878exonuclease SbcD 
9VPA10241078504..1079346 [-], 84328900879hypothetical protein 
10VPA10251079347..1079637 [-], 29128900880hypothetical protein  PAAR
11VPA10261079651..1081486 [-], 183628900881hypothetical protein  TssI
12VPA10271081544..1082023 [-], 48028900882hypothetical protein  TssD
13VPA10281082229..1084796 [-], 256828900883ClpA/B-type chaperone  TssH
14VPA10291084807..1085772 [-], 96628900884hypothetical protein  TssG
15VPA10301085769..1087592 [-], 182428900885hypothetical protein  TssF
16VPA10311087589..1088053 [-], 46528900886hypothetical protein  TssE
17VPA10321088062..1088859 [-], 79828900887hypothetical protein  TagJ
18VPA10331088870..1090390 [-], 152128900888hypothetical protein  TssC
19VPA10341090437..1091930 [-], 149428900889hypothetical protein  TssC
20VPA10351091930..1092460 [-], 53128900890hypothetical protein  TssB
21VPA10361092476..1093597 [-], 112228900891hypothetical protein  TssA
22VPA10371093594..1094382 [-], 78928900892phosphoprotein phosphatase 
23VPA10381094393..1095088 [-], 69628900893hypothetical protein 
24VPA10391095070..1098588 [-], 351928900894hypothetical protein  TssM
25VPA10401098604..1099905 [-], 130228900895hypothetical protein  TssL
26VPA10411099908..1101233 [-], 132628900896hypothetical protein  TssK
27VPA10421101255..1101710 [-], 45628900897hypothetical protein  TssJ
28VPA10431101720..1102916 [-], 119728900898hypothetical protein  Fha
29VPA10441103236..1105380 [+], 214528900899hypothetical protein 
30VPA10451105492..1107027 [+], 153628900900hypothetical protein 
31VPA10461107024..1107365 [+], 34228900901hypothetical protein 
32VPA10471107457..1107588 [-], 13228900902hypothetical protein 
33VPA10481107620..1108114 [-], 49528900903anti-sigma F factor antagonist 
34VPA10491108107..1109327 [-], 122128900904two-component response regulator 
35VPA10501109352..1109675 [-], 32428900905hypothetical protein 
36VPA10511109834..1110448 [-], 61528900906glutathione S-transferase 
37VPA10521110593..1111168 [+], 57628900907TetR family regulatory protein 
38VPA10531111170..1111574 [+], 40528900908hypothetical protein 
39VPA10541111677..1112012 [-], 33628900909nitrite reductase NADPH (small subunit) oxidoreductase protein 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1VPA10271081544..1082023 [-], 48028900882hypothetical protein VPA1027
Data from experimental literature

Download FASTA format files
Proteins        Genes
Regulatory element identified
OpaR (VP2516)ActivatorPMID: 23613791

VPA1045ActivatorPMID: 23173431

VPA1049ActivatorPMID: 23173431

AphARepressorPMID: 23977385

The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1VP0350 (CalR/LeuO)VP1133 (H-NS).
2VP0350 (CalR/LeuO)Transcription regulator+VPA1027 (hcp2) promotorCalR activates the promoter activities of T6SS2 genes,and antagonise H-NS.
3VP0350 (CalR/LeuO)VPA1043 (Fha) promotor.
4VP1241 (TfoX)QS regulator+T6SS2TfoX specifically induces T6SS2.
(1) Yu Y et al. (2015). VgrG2 of type VI secretion system 2 of Vibrio parahaemolyticus induces autophagy in macrophages. Front Microbiol. 6:168. [PudMed:25784905] experimental
(2) Wang L et al. (2013). Cell Density- and Quorum Sensing-Dependent Expression of Type VI Secretion System 2 in Vibrio parahaemolyticus. PLoS One. 8(8):e73363. [PudMed:23977385] experimental
(3) Salomon D et al. (2013). Vibrio parahaemolyticus Type VI Secretion System 1 Is Activated in Marine Conditions to Target Bacteria, and Is Differentially Regulated from System 2. PLoS One. 8(4):e61086. [PudMed:23613791] experimental
(4) Yu Y et al. (2012). VPA1045 and VPA1049 of Vibrio parahaemolyticus regulate translocation of Hcp2. Wei Sheng Wu Xue Bao. 52(8):954-61. [PudMed:23173431] experimental
(5) Ma L et al. (2012). Expression of the Type VI Secretion System 1 Component Hcp1 Is Indirectly Repressed by OpaR in Vibrio parahaemolyticus. Scientific World Journal. 2012:982140. [PudMed:22924031] experimental
(6) Yu Y et al. (2012). Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers. Arch Microbiol. 194(10):827-35. [PudMed:22535222] experimental
(7) Boyer F et al. (2009). Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 10:104. [PudMed:19284603] in_silico
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation