I. Summary

SecReT6 ID	443
Strain	Methylomonas methanica MC09
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_015572
Location	915765..926524
Comment
Function	effector translocation
T6SS Type	Type i; subtype i3
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	0	0	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	0	0

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	METME_RS04275	911243..912682 [+], 1440	503583475	F0F1 ATP synthase subunit alpha
2	METME_RS04280	912679..913491 [+], 813	503583476	F0F1 ATP synthase subunit gamma
3	METME_RS04285	913546..914016 [-], 471	908658285	hypothetical protein
4	METME_RS04290	914136..914531 [+], 396	pseudogene	hypothetical protein
5	METME_RS04295	914912..915655 [+], 744	503583479	response regulator
6	METME_RS04300 (tssA)	915765..916805 [+], 1041	503583480	type VI secretion system protein TssA
7	METME_RS04305 (tssB)	916832..917368 [+], 537	503583481	type VI secretion system contractile sheath small subunit
8	METME_RS04310 (tssC)	917372..918862 [+], 1491	503583482	type VI secretion system contractile sheath large subunit
9	METME_RS04315	918983..919465 [+], 483	503583483	type VI secretion system tube protein Hcp
10	METME_RS04320	919535..920344 [+], 810	503583484	virulence protein SciE type
11	METME_RS04325 (tssE)	920347..920850 [+], 504	503583485	type VI secretion system baseplate subunit TssE
12	METME_RS04330 (tssF)	920853..922736 [+], 1884	503583486	type VI secretion system baseplate subunit TssF
13	METME_RS04335 (tssG)	922700..923764 [+], 1065	503583487	type VI secretion system baseplate subunit TssG
14	METME_RS04340 (tssH)	923804..926524 [+], 2721	503583488	type VI secretion system ATPase TssH
15	METME_RS04345	926790..927809 [+], 1020	503583489	hypothetical protein
16	METME_RS04350	927880..928224 [+], 345	752716621	hypothetical protein
17	METME_RS04355	928491..929525 [+], 1035	503583491	hypothetical protein
18	METME_RS04360	929576..929917 [+], 342	503583492	hypothetical protein
19	METME_RS04365	930046..930384 [+], 339	752717890	PQQ-binding-like beta-propeller repeat protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PA2702

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

Homology Immunity protein list

Metme_3456 (Tsi2_MM)

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

(1) Silverman JM et al. (2013). Haemolysin Coregulated Protein Is an Exported Receptor and Chaperone of Type VI Secretion Substrates. Mol Cell. 51(5):584-93. [PudMed:23954347]

This literature contains experimental investigation

This literature contains bioinformatics investigation