SecReT6
I. Summary
SecReT6 ID463 experimental
StrainEscherichia coli 042
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_017626
Location4852875..4885437
Comment
Functioneffector translocation
T6SS TypeType i; subtype i2
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number111121111
ComponentTssGTssJTssKTssM
Number1111
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1EC042_RS24130 (hha)4848505..4848717 [+], 213446375476hemolysin expression modulator Hha 
2EC042_RS241354849334..4849903 [+], 570446142872inovirus Gp2 family protein 
3EC042_RS307304849941..4850099 [+], 159446415670hypothetical protein 
4EC042_RS241404850071..4850454 [+], 384446193167hypothetical protein 
5EC042_RS241454850444..4850863 [+], 420485761816hypothetical protein 
6EC042_RS241504850869..4851051 [+], 183447139057hypothetical protein 
7EC042_RS241554851172..4851795 [+], 624447183010hypothetical protein 
8EC042_RS241604851891..4852124 [+], 234446046326hypothetical protein 
9EC042_RS241654852177..4852368 [-], 192447210540DUF4222 domain-containing protein 
10EC042_RS24170 (tssB)4852875..4853372 [+], 498446021084type VI secretion system contractile sheath small subunit 
11EC042_RS24175 (tssC)4853394..4854938 [+], 1545446852935type VI secretion system contractile sheath large subunit 
12EC042_RS24180 (tssK)4854954..4856291 [+], 1338446631292type VI secretion system baseplate subunit TssK 
13EC042_RS241854856288..4856941 [+], 654446962789type VI secretion system protein TssL, short form 
14EC042_RS241904856944..4858674 [+], 1731446480629OmpA family protein 
15EC042_RS24195 (hcp)4858680..4859171 [+], 492446930057type VI secretion system effector Hcp 
16EC042_RS24200 (tssH)4859340..4861997 [+], 2658446353791type VI secretion system ATPase TssH 
17EC042_RS242054861994..4862560 [+], 567446070506hypothetical protein 
18EC042_RS288504862554..4863096 [+], 543485781044hypothetical protein 
19EC042_RS24215 (vgrG)4863083..4865608 [+], 2526446977247type VI secretion system tip protein VgrG 
20EC042_RS24220 (tle1)4865605..4867287 [+], 1683446092701T6SS effector phospholipase Tle1-EAEC 
21EC042_RS24225 (tli1)4867307..4867984 [+], 678446118504T6SS immunity phospholipase A1-binding lipoprotein Tli1-EAEC 
22EC042_RS24230 (tli1)4868128..4868805 [+], 678446118503T6SS immunity phospholipase A1-binding lipoprotein Tli1-EAEC 
23EC042_RS242354868837..4869103 [+], 267445955555PAAR domain-containing protein 
24EC042_RS242404869107..4870255 [+], 1149485781045hypothetical protein 
25EC042_RS242454870248..4873637 [+], 3390446959832type VI secretion protein VasK 
26EC042_RS24250 (tssA)4873637..4875235 [+], 1599446068660type VI secretion system protein TssA 
27EC042_RS242554875237..4875929 [+], 693446037502hypothetical protein 
28EC042_RS24260 (tssF)4875937..4877700 [+], 1764446264608type VI secretion system baseplate subunit TssF 
29EC042_RS24265 (tssG)4877655..4878755 [+], 1101446475927type VI secretion system baseplate subunit TssG 
30EC042_RS24270 (tssJ)4878736..4879272 [+], 537446897213type VI secretion system lipoprotein TssJ 
31EC042_RS24275 (tssE)4879275..4879706 [+], 432447060676type VI secretion system baseplate subunit TssE 
32EC042_RS242804879863..4880264 [+], 402485781047hypothetical protein 
33EC042_RS242854880420..4881664 [+], 1245446819776GIY-YIG nuclease family protein 
34EC042_RS242904881900..4883645 [+], 1746586706166AAA family ATPase 
35EC042_RS243004884070..4885437 [+], 1368446122356type VI secretion system ImpA family N-terminal domain-containing protein 
36EC042_RS243054885652..4886212 [+], 561446508647hypothetical protein 
37EC042_RS243104886215..4887045 [+], 831446293040hypothetical protein 
38EC042_RS243154887086..4888318 [+], 1233447127386phosphoadenosine phosphosulfate reductase 
39EC042_RS243204888303..4888947 [+], 645446425008ParB N-terminal domain-containing protein 
40EC042_RS243254889026..4889295 [+], 270446148662PerC family transcriptional regulator 
41EC042_RS243304889316..4889960 [+], 645485761812hypothetical protein 
42EC042_RS243354890026..4890349 [+], 324485761811hypothetical protein 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
EC042_4529

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes

Homology Immunity protein list
EC042_4535

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1EC042_RS24225(tli1)4867307..4867984 [+], 678446118504T6SS immunity phospholipase A1-binding lipoprotein Tli1-EAEC EC042_4535
Data from experimental literature

Download FASTA format files
Proteins        Genes
(1) Zoued A et al. (2013). TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the Type VI secretion system. J Biol Chem. 288(38):27031-41. [PudMed:23921384] experimental
(2) Morin N et al. (2013). Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 81(1):122-32. [PudMed:23090962] experimental
(3) Durand E et al. (2012). Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. J Biol Chem. 287(17):14157-68. [PudMed:22371492]
(4) Felisberto-Rodrigues C et al. (2011). Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 7(11):e1002386. [PudMed:22102820]
(5) Dudley EG et al. (2006). Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 61(5):1267-82. [PudMed:16925558] experimental
 
experimental This literature contains experimental investigation