I. Summary

SecReT6 ID	463
Strain	Escherichia coli 042
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_017626
Location	4852875..4885437
Comment
Function	effector translocation
T6SS Type	Type i; subtype i2
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	EC042_4516	4848505..4848717 [+], 213	387609939	putative plasmid-related regulatory protein
2	EC042_4518	4849334..4849903 [+], 570	387609940	hypothetical protein
3	EC042_4519	4850065..4850454 [+], 390	387609941	hypothetical protein
4	EC042_4521	4851172..4851795 [+], 624	387609942	hypothetical protein
5	EC042_4522	4851891..4852124 [+], 234	387609943	hypothetical protein
6	EC042_4523	4852177..4852368 [-], 192	387609944	hypothetical protein
7	EC042_4524	4852875..4853372 [+], 498	387609945	putative type VI secretion protein	TssB
8	EC042_4525	4853394..4854938 [+], 1545	387609946	putative type VI secretion protein	TssC
9	EC042_4526	4854954..4856291 [+], 1338	387609947	putative type VI secretion protein	TssK
10	EC042_4527	4856288..4856941 [+], 654	387609948	putative type VI secretion protein	TssL
11	EC042_4528	4856944..4858674 [+], 1731	387609949	putative type VI secretion protein	TagL
12	EC042_4529	4858680..4859171 [+], 492	387609950	putative type VI secretion protein	TssD
13	EC042_4530	4859340..4861997 [+], 2658	387609951	putative type VI secretion protein	TssH
14	EC042_4531	4861994..4862560 [+], 567	387609952	putative type VI secretion protein
15	EC042_4532	4862554..4863096 [+], 543	387609953	putative type VI secretion protein
16	EC042_4533	4863083..4865608 [+], 2526	387609954	putative type VI secretion protein	TssI
17	EC042_4534	4865605..4867287 [+], 1683	387609955	putative type VI secretion protein
18	EC042_4535	4867307..4867984 [+], 678	387609956	putative type VI secretion protein
19	EC042_4536	4868128..4868805 [+], 678	387609957	putative type VI secretion protein
20	EC042_4537	4868837..4869103 [+], 267	387609958	putative type VI secretion protein	PAAR
21	EC042_4538	4869107..4870255 [+], 1149	387609959	putative type VI secretion protein
22	EC042_4539	4870248..4873637 [+], 3390	387609960	putative type VI secretion protein	TssM
23	EC042_4540	4873637..4875235 [+], 1599	387609961	putative type VI secretion protein	TssA
24	EC042_4541	4875237..4875929 [+], 693	387609962	putative type VI secretion protein
25	EC042_4542	4875937..4877700 [+], 1764	387609963	putative type VI secretion protein	TssF
26	EC042_4543	4877754..4878755 [+], 1002	387609964	putative type VI secretion protein	TssG
27	EC042_4544	4878736..4879272 [+], 537	387609965	putative type VI secretion protein	TssJ
28	EC042_4545	4879275..4879706 [+], 432	387609966	putative type VI secretion protein	TssE
29	EC042_4546	4879851..4880264 [+], 414	387609967	hypothetical protein
30	EC042_4547	4880420..4881664 [+], 1245	387609968	hypothetical protein
31	EC042_4548	4881858..4883645 [+], 1788	387609969	hypothetical protein
32	EC042_4549	4883710..4884015 [+], 306	387609970	hypothetical protein
33	EC042_4550	4884070..4885437 [+], 1368	387609971	hypothetical protein	TssA
34	EC042_4551	4885652..4886212 [+], 561	387609972	hypothetical protein
35	EC042_4552	4886215..4887045 [+], 831	387609973	hypothetical protein
36	EC042_4553	4887086..4888318 [+], 1233	387609974	hypothetical protein
37	EC042_4554	4888303..4888947 [+], 645	387609975	ParB-like nuclease
38	EC042_4555	4889026..4889295 [+], 270	387609976	putative transcriptional regulator
39	EC042_4556	4889316..4889960 [+], 645	387609977	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

EC042_4529

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	EC042_4529	4858680..4859171 [+], 492	387609950	putative type VI secretion protein	EC042_4529
Data from experimental literature

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Proteins        Genes

Homology Immunity protein list

EC042_4535

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

(1) Zoued A et al. (2013). TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the Type VI secretion system. J Biol Chem. 288(38):27031-41. [PudMed:23921384]

(2) Morin N et al. (2013). Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 81(1):122-32. [PudMed:23090962]

(3) Durand E et al. (2012). Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. J Biol Chem. 287(17):14157-68. [PudMed:22371492]

(4) Felisberto-Rodrigues C et al. (2011). Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 7(11):e1002386. [PudMed:22102820]

(5) Dudley EG et al. (2006). Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 61(5):1267-82. [PudMed:16925558]

This literature contains experimental investigation