I. Summary

SecReT6 ID	463
Strain	Escherichia coli 042
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_017626
Location	4852875..4885437
Comment
Function	effector translocation
T6SS Type	Type i; subtype i2
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	EC042_RS24130 (hha)	4848505..4848717 [+], 213	446375476	hemolysin expression modulator Hha
2	EC042_RS24135	4849334..4849903 [+], 570	446142872	inovirus Gp2 family protein
3	EC042_RS30730	4849941..4850099 [+], 159	446415670	hypothetical protein
4	EC042_RS24140	4850071..4850454 [+], 384	446193167	hypothetical protein
5	EC042_RS24145	4850444..4850863 [+], 420	485761816	hypothetical protein
6	EC042_RS24150	4850869..4851051 [+], 183	447139057	hypothetical protein
7	EC042_RS24155	4851172..4851795 [+], 624	447183010	hypothetical protein
8	EC042_RS24160	4851891..4852124 [+], 234	446046326	hypothetical protein
9	EC042_RS24165	4852177..4852368 [-], 192	447210540	DUF4222 domain-containing protein
10	EC042_RS24170 (tssB)	4852875..4853372 [+], 498	446021084	type VI secretion system contractile sheath small subunit
11	EC042_RS24175 (tssC)	4853394..4854938 [+], 1545	446852935	type VI secretion system contractile sheath large subunit
12	EC042_RS24180 (tssK)	4854954..4856291 [+], 1338	446631292	type VI secretion system baseplate subunit TssK
13	EC042_RS24185	4856288..4856941 [+], 654	446962789	type VI secretion system protein TssL, short form
14	EC042_RS24190	4856944..4858674 [+], 1731	446480629	OmpA family protein
15	EC042_RS24195 (hcp)	4858680..4859171 [+], 492	446930057	type VI secretion system effector Hcp
16	EC042_RS24200 (tssH)	4859340..4861997 [+], 2658	446353791	type VI secretion system ATPase TssH
17	EC042_RS24205	4861994..4862560 [+], 567	446070506	hypothetical protein
18	EC042_RS28850	4862554..4863096 [+], 543	485781044	hypothetical protein
19	EC042_RS24215 (vgrG)	4863083..4865608 [+], 2526	446977247	type VI secretion system tip protein VgrG
20	EC042_RS24220 (tle1)	4865605..4867287 [+], 1683	446092701	T6SS effector phospholipase Tle1-EAEC
21	EC042_RS24225 (tli1)	4867307..4867984 [+], 678	446118504	T6SS immunity phospholipase A1-binding lipoprotein Tli1-EAEC
22	EC042_RS24230 (tli1)	4868128..4868805 [+], 678	446118503	T6SS immunity phospholipase A1-binding lipoprotein Tli1-EAEC
23	EC042_RS24235	4868837..4869103 [+], 267	445955555	PAAR domain-containing protein
24	EC042_RS24240	4869107..4870255 [+], 1149	485781045	hypothetical protein
25	EC042_RS24245	4870248..4873637 [+], 3390	446959832	type VI secretion protein VasK
26	EC042_RS24250 (tssA)	4873637..4875235 [+], 1599	446068660	type VI secretion system protein TssA
27	EC042_RS24255	4875237..4875929 [+], 693	446037502	hypothetical protein
28	EC042_RS24260 (tssF)	4875937..4877700 [+], 1764	446264608	type VI secretion system baseplate subunit TssF
29	EC042_RS24265 (tssG)	4877655..4878755 [+], 1101	446475927	type VI secretion system baseplate subunit TssG
30	EC042_RS24270 (tssJ)	4878736..4879272 [+], 537	446897213	type VI secretion system lipoprotein TssJ
31	EC042_RS24275 (tssE)	4879275..4879706 [+], 432	447060676	type VI secretion system baseplate subunit TssE
32	EC042_RS24280	4879863..4880264 [+], 402	485781047	hypothetical protein
33	EC042_RS24285	4880420..4881664 [+], 1245	446819776	GIY-YIG nuclease family protein
34	EC042_RS24290	4881900..4883645 [+], 1746	586706166	AAA family ATPase
35	EC042_RS24300	4884070..4885437 [+], 1368	446122356	type VI secretion system ImpA family N-terminal domain-containing protein
36	EC042_RS24305	4885652..4886212 [+], 561	446508647	hypothetical protein
37	EC042_RS24310	4886215..4887045 [+], 831	446293040	hypothetical protein
38	EC042_RS24315	4887086..4888318 [+], 1233	447127386	phosphoadenosine phosphosulfate reductase
39	EC042_RS24320	4888303..4888947 [+], 645	446425008	ParB N-terminal domain-containing protein
40	EC042_RS24325	4889026..4889295 [+], 270	446148662	PerC family transcriptional regulator
41	EC042_RS24330	4889316..4889960 [+], 645	485761812	hypothetical protein
42	EC042_RS24335	4890026..4890349 [+], 324	485761811	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

EC042_4529

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

Homology Immunity protein list

EC042_4535

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	EC042_RS24225(tli1)	4867307..4867984 [+], 678	446118504	T6SS immunity phospholipase A1-binding lipoprotein Tli1-EAEC	EC042_4535
Data from experimental literature

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Proteins        Genes

(1) Zoued A et al. (2013). TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the Type VI secretion system. J Biol Chem. 288(38):27031-41. [PudMed:23921384]

(2) Morin N et al. (2013). Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 81(1):122-32. [PudMed:23090962]

(3) Durand E et al. (2012). Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. J Biol Chem. 287(17):14157-68. [PudMed:22371492]

(4) Felisberto-Rodrigues C et al. (2011). Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 7(11):e1002386. [PudMed:22102820]

(5) Dudley EG et al. (2006). Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 61(5):1267-82. [PudMed:16925558]

This literature contains experimental investigation