SecReT6
I. Summary
SecReT6 ID466 experimental
StrainFrancisella tularensis subsp. holarctica LVS
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_007880
Location102157..124300
CommentGrowth, phagosomal escape and cytopathogenicity in cultured macrophages; virulence in mouse model
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType ii (Type FPI)
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentPdpAPdpBIglEVgrGIglFIglGIglHDotUIglI
Number111111111
ComponentIglJPdpCPdpEIglDIglCIglBIglAPdpD
Number11111112
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1FTL_RS0050598287..98684 [-], 398pseudogeneDUF3568 domain-containing protein 
2FTL_RS0051098687..99097 [-], 411489104225DUF3568 domain-containing protein 
3FTL_RS0051599118..99510 [-], 393489104226DUF3568 domain-containing protein 
4FTL_RS0052099799..100251 [+], 453pseudogenenicotinamide mononucleotide transporter 
5FTL_RS10125100311..101191 [+], 881499776592IS630 family transposase 
6FTL_RS00535101192..102064 [-], 873499776609MFS transporter 
7FTL_RS10795102049..102771 [+], 723pseudogenePathogenicity determinant protein D 
8FTL_RS00550 (tssB)102852..103406 [+], 555489106300type VI secretion system contractile sheath small subunit 
9FTL_RS00555 (tssC)103420..104940 [+], 1521497717462type VI secretion system contractile sheath large subunit 
10FTL_RS00560104957..105586 [+], 630489106304hypothetical protein 
11FTL_RS00565105607..106803 [+], 1197499776611hypothetical protein 
12FTL_RS00570106810..107385 [-], 576489106308hypothetical protein 
13FTL_RS00575 (pdpC)107403..111389 [-], 3987489106311pathogenicity determinant protein PdpC 
14FTL_RS00580111429..112202 [-], 774489106314hypothetical protein 
15FTL_RS00585112168..113319 [-], 1152489106318hypothetical protein 
16FTL_RS00590113341..113964 [-], 624489106320DotU family type IV/VI secretion system protein 
17FTL_RS00595113977..115407 [-], 1431489106321hypothetical protein 
18FTL_RS00600115420..115941 [-], 522489106324DUF4280 domain-containing protein 
19FTL_RS00605116009..117673 [-], 1665489106327hypothetical protein 
20FTL_RS00610117678..118172 [-], 495489106331hypothetical protein 
21FTL_RS00615118185..118562 [-], 378489108377hypothetical protein 
22FTL_RS00620118552..121833 [-], 3282499776612hypothetical protein 
23FTL_RS00625121838..124300 [-], 2463489106334hypothetical protein 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
FTL_0118 (IglI) , FTT_1347 (VgrG)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes
N/A
Regulatory element identified
SspA
RegulatorDescriptionReference
SspA (FTL_1606)Indirect activatorPMID: 19876386

PigR
RegulatorDescriptionReference
PigRIndirect activatorPMID: 19876386

MigR
RegulatorDescriptionReference
MigR (FTL_1542)Indirect activatorPMID: 19349423

FevR
RegulatorDescriptionReference
FevR (FTL_0449)ActivatorPMID: 19349423

MglA
RegulatorDescriptionReference
MglA (FTL_1185)ActivatorPMID: 17000729

Hfq
RegulatorDescriptionReference
HfqRepressorPMID: 19223477

(1) Lindgren M et al. (2013). Importance of PdpC, IglC, IglI, and IglG for modulation of a host cell death pathway induced by Francisella tularensis LVS. Infect Immun. 81(6):2076-84. [PudMed:23529623] experimental
(2) Lindgren M et al. (2013). The Francisella tularensis LVS DeltapdpC mutant exhibits a unique phenotype during intracellular infection. BMC Microbiol. 13(1):20. [PudMed:23356941] experimental
(3) Broms JE et al. (2012). Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631] experimental
(4) Broms JE et al. (2012). DotU and VgrG, core components of type VI secretion systems, are essential for Francisella LVS pathogenicity. PLoS One. 7(4):e34639. [PudMed:22514651] experimental
(5) de Bruin OM et al. (2011). The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115] experimental
(6) Broms JE et al. (2011). IglG and IglI of the Francisella pathogenicity island are important virulence determinants of Francisella tularensis LVS. Infect Immun. 79(9):3683-96. [PudMed:21690239] experimental
(7) Nano FE et al. (2007). The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]
 
experimental This literature contains experimental investigation