SecReT6
I. Summary
SecReT6 ID466 experimental
StrainFrancisella tularensis subsp. holarctica LVS
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_007880
Location102157..124300
CommentGrowth, phagosomal escape and cytopathogenicity in cultured macrophages; virulence in mouse model
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType ii (Type FPI)
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentPdpAPdpBIglEVgrGIglFIglGIglHDotUIglI
Number111111111
ComponentIglJPdpCPdpEIglDIglCIglBIglAPdpD
Number11111112
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1FTL_010297893..98216 [-], 32489255540hypothetical protein 
2FTL_010398400..98684 [-], 28589255541hypothetical protein 
3FTL_010498687..99097 [-], 41189255542hypothetical protein 
4FTL_010599118..99510 [-], 39389255543hypothetical protein 
5FTL_0107 (isftu1)100311..101191 [+], 88189255544transposase 
6FTL_0108101192..102064 [-], 87389255545hypothetical protein 
7FTL_0109102157..102558 [+], 40289255546hypothetical protein  PdpD
8FTL_0110102568..102771 [+], 20489255547hypothetical protein  PdpD
9FTL_0111102852..103406 [+], 55589255548intracellular growth locus, subunit A  IglA
10FTL_0112103420..104940 [+], 152189255549intracellular growth locus, subunit B  IglB
11FTL_0113104957..105586 [+], 63089255550intracellular growth locus, subunit C  IglC
12FTL_0114105607..106803 [+], 119789255551intracellular growth locus, subunit D  IglD
13FTL_0115106810..107385 [-], 57689255552hypothetical protein  PdpE
14FTL_0116107403..111389 [-], 398789255553hypothetical protein  PdpC
15FTL_0117111429..112202 [-], 77489255554hypothetical protein  IglJ
16FTL_0118112168..113319 [-], 115289255555hypothetical protein  IglI
17FTL_0119113341..113964 [-], 62489255556hypothetical protein  DotU
18FTL_0120113977..115407 [-], 143189255557hypothetical protein  IglH
19FTL_0121115420..115941 [-], 52289255558hypothetical protein  IglG
20FTL_0122116009..117673 [-], 166589255559hypothetical protein  IglF
21FTL_0123117678..118172 [-], 49589255560hypothetical protein  VgrG
22FTL_0124118185..118562 [-], 37889255561hypothetical protein  IglE
23FTL_0125118552..121833 [-], 328289255562hypothetical protein  PdpB
24FTL_0126121838..124300 [-], 246389255563hypothetical protein  PdpA
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
FTL_0118 (IglI) , FTT_1347 (VgrG)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1FTL_0118112168..113319 [-], 115289255555hypothetical protein (IglI)FTL_0118 (IglI)
2FTL_0123117678..118172 [-], 49589255560hypothetical protein (VgrG)FTT_1347 (VgrG)
Data from experimental literature

Download FASTA format files
Proteins        Genes
N/A
Regulatory element identified
SspA
RegulatorDescriptionReference
SspA (FTL_1606)Indirect activatorPMID: 19876386

PigR
RegulatorDescriptionReference
PigRIndirect activatorPMID: 19876386

MigR
RegulatorDescriptionReference
MigR (FTL_1542)Indirect activatorPMID: 19349423

FevR
RegulatorDescriptionReference
FevR (FTL_0449)ActivatorPMID: 19349423

MglA
RegulatorDescriptionReference
MglA (FTL_1185)ActivatorPMID: 17000729

Hfq
RegulatorDescriptionReference
HfqRepressorPMID: 19223477

(1) Lindgren M et al. (2013). Importance of PdpC, IglC, IglI, and IglG for modulation of a host cell death pathway induced by Francisella tularensis LVS. Infect Immun. 81(6):2076-84. [PudMed:23529623] experimental
(2) Lindgren M et al. (2013). The Francisella tularensis LVS DeltapdpC mutant exhibits a unique phenotype during intracellular infection. BMC Microbiol. 13(1):20. [PudMed:23356941] experimental
(3) Broms JE et al. (2012). Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631] experimental
(4) Broms JE et al. (2012). DotU and VgrG, core components of type VI secretion systems, are essential for Francisella LVS pathogenicity. PLoS One. 7(4):e34639. [PudMed:22514651] experimental
(5) de Bruin OM et al. (2011). The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115] experimental
(6) Broms JE et al. (2011). IglG and IglI of the Francisella pathogenicity island are important virulence determinants of Francisella tularensis LVS. Infect Immun. 79(9):3683-96. [PudMed:21690239] experimental
(7) Nano FE et al. (2007). The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]
 
experimental This literature contains experimental investigation