I. Summary

SecReT6 ID	466
Strain	Francisella tularensis subsp. holarctica LVS
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_007880
Location	102157..124300
Comment	Growth, phagosomal escape and cytopathogenicity in cultured macrophages; virulence in mouse model
Function	anti-eukaryotic; effector translocation;virulence
T6SS Type	Type ii (Type FPI)
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	PdpA	PdpB	IglE	VgrG	IglF	IglG	IglH	DotU	IglI
Number	1	1	1	1	1	1	1	1	1
Component	IglJ	PdpC	PdpE	IglD	IglC	IglB	IglA	PdpD
Number	1	1	1	1	1	1	1	2

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	FTL_0102	97893..98216 [-], 324	89255540	hypothetical protein
2	FTL_0103	98400..98684 [-], 285	89255541	hypothetical protein
3	FTL_0104	98687..99097 [-], 411	89255542	hypothetical protein
4	FTL_0105	99118..99510 [-], 393	89255543	hypothetical protein
5	FTL_0107 (isftu1)	100311..101191 [+], 881	89255544	transposase
6	FTL_0108	101192..102064 [-], 873	89255545	hypothetical protein
7	FTL_0109	102157..102558 [+], 402	89255546	hypothetical protein	PdpD
8	FTL_0110	102568..102771 [+], 204	89255547	hypothetical protein	PdpD
9	FTL_0111	102852..103406 [+], 555	89255548	intracellular growth locus, subunit A	IglA
10	FTL_0112	103420..104940 [+], 1521	89255549	intracellular growth locus, subunit B	IglB
11	FTL_0113	104957..105586 [+], 630	89255550	intracellular growth locus, subunit C	IglC
12	FTL_0114	105607..106803 [+], 1197	89255551	intracellular growth locus, subunit D	IglD
13	FTL_0115	106810..107385 [-], 576	89255552	hypothetical protein	PdpE
14	FTL_0116	107403..111389 [-], 3987	89255553	hypothetical protein	PdpC
15	FTL_0117	111429..112202 [-], 774	89255554	hypothetical protein	IglJ
16	FTL_0118	112168..113319 [-], 1152	89255555	hypothetical protein	IglI
17	FTL_0119	113341..113964 [-], 624	89255556	hypothetical protein	DotU
18	FTL_0120	113977..115407 [-], 1431	89255557	hypothetical protein	IglH
19	FTL_0121	115420..115941 [-], 522	89255558	hypothetical protein	IglG
20	FTL_0122	116009..117673 [-], 1665	89255559	hypothetical protein	IglF
21	FTL_0123	117678..118172 [-], 495	89255560	hypothetical protein	VgrG
22	FTL_0124	118185..118562 [-], 378	89255561	hypothetical protein	IglE
23	FTL_0125	118552..121833 [-], 3282	89255562	hypothetical protein	PdpB
24	FTL_0126	121838..124300 [-], 2463	89255563	hypothetical protein	PdpA

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

FTL_0118 (IglI) , FTT_1347 (VgrG)

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	FTL_0118	112168..113319 [-], 1152	89255555	hypothetical protein (IglI)	FTL_0118 (IglI)
2	FTL_0123	117678..118172 [-], 495	89255560	hypothetical protein (VgrG)	FTT_1347 (VgrG)
Data from experimental literature

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Proteins        Genes

N/A

Regulatory element identified

SspA

Regulator	Description	Reference
SspA (FTL_1606)	Indirect activator	PMID: 19876386

PigR

Regulator	Description	Reference
PigR	Indirect activator	PMID: 19876386

MigR

Regulator	Description	Reference
MigR (FTL_1542)	Indirect activator	PMID: 19349423

FevR

Regulator	Description	Reference
FevR (FTL_0449)	Activator	PMID: 19349423

MglA

Regulator	Description	Reference
MglA (FTL_1185)	Activator	PMID: 17000729

Hfq

Regulator	Description	Reference
Hfq	Repressor	PMID: 19223477

(1) Lindgren M et al. (2013). Importance of PdpC, IglC, IglI, and IglG for modulation of a host cell death pathway induced by Francisella tularensis LVS. Infect Immun. 81(6):2076-84. [PudMed:23529623]

(2) Lindgren M et al. (2013). The Francisella tularensis LVS DeltapdpC mutant exhibits a unique phenotype during intracellular infection. BMC Microbiol. 13(1):20. [PudMed:23356941]

(3) Broms JE et al. (2012). Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631]

(4) Broms JE et al. (2012). DotU and VgrG, core components of type VI secretion systems, are essential for Francisella LVS pathogenicity. PLoS One. 7(4):e34639. [PudMed:22514651]

(5) de Bruin OM et al. (2011). The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115]

(6) Broms JE et al. (2011). IglG and IglI of the Francisella pathogenicity island are important virulence determinants of Francisella tularensis LVS. Infect Immun. 79(9):3683-96. [PudMed:21690239]

(7) Nano FE et al. (2007). The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]

This literature contains experimental investigation