I. Summary

SecReT6 ID	467
Strain	Francisella tularensis subsp. holarctica LVS
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_007880
Location	1096796..1118939
Comment	Growth, phagosomal escape and cytopathogenicity in cultured macrophages; virulence in mouse model
Function	anti-eukaryotic; effector translocation;virulence
T6SS Type	Type ii (Type FPI)
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	PdpA	PdpB	IglE	VgrG	IglF	IglG	IglH	DotU	IglI
Number	1	1	1	1	1	1	1	1	1
Component	IglJ	PdpC	PdpE	IglD	IglC	IglB	IglA	PdpD
Number	1	1	1	1	1	1	1	2

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	FTL_1149	1091858..1092922 [+], 1065	89256473	fructose-1,6-bisphosphate aldolase
2	FTL_1150	1093090..1093644 [-], 555	89256474	Alpha,alpha-trehalase.
3	FTL_1151	1093524..1094570 [-], 1047	89256475	trehalase, pseuodgene
4	FTL_1153 (isftu1)	1094950..1095830 [+], 881	89256476	transposase
5	FTL_1155	1096796..1097197 [+], 402	89256477	hypothetical protein	PdpD
6	FTL_1156	1097207..1097410 [+], 204	89256478	hypothetical protein	PdpD
7	FTL_1157	1097491..1098045 [+], 555	89256479	intracellular growth locus, subunit A	IglA
8	FTL_1158	1098059..1099579 [+], 1521	89256480	intracellular growth locus, subunit B	IglB
9	FTL_1159	1099596..1100225 [+], 630	89256481	intracellular growth locus, subunit C	IglC
10	FTL_1160	1100246..1101442 [+], 1197	89256482	intracellular growth locus, subunit D	IglD
11	FTL_1161	1101449..1102024 [-], 576	89256483	hypothetical protein	PdpE
12	FTL_1162	1102042..1106028 [-], 3987	89256484	hypothetical protein	PdpC
13	FTL_1163	1106068..1106841 [-], 774	89256485	hypothetical protein	IglJ
14	FTL_1164	1106807..1107958 [-], 1152	89256486	hypothetical protein	IglI
15	FTL_1165	1107980..1108603 [-], 624	89256487	hypothetical protein	DotU
16	FTL_1166	1108616..1110046 [-], 1431	89256488	hypothetical protein	IglH
17	FTL_1167	1110059..1110580 [-], 522	89256489	hypothetical protein	IglG
18	FTL_1168	1110648..1112312 [-], 1665	89256490	hypothetical protein	IglF
19	FTL_1169	1112317..1112811 [-], 495	89256491	hypothetical protein	VgrG
20	FTL_1170	1112824..1113201 [-], 378	89256492	hypothetical protein	IglE
21	FTL_1171	1113191..1116472 [-], 3282	89256493	hypothetical protein	PdpB
22	FTL_1172	1116477..1118939 [-], 2463	89256494	hypothetical protein	PdpA

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

FTL_0118 (IglI) , FTT_1347 (VgrG)

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	FTL_1164	1106807..1107958 [-], 1152	89256486	hypothetical protein (IglI)	FTL_0118 (IglI)
2	FTL_1169	1112317..1112811 [-], 495	89256491	hypothetical protein (VgrG)	FTT_1347 (VgrG)
Data from experimental literature

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Proteins        Genes

N/A

Regulatory element identified

SspA

Regulator	Description	Reference
SspA (FTL_1606)	Indirect activator	PMID: 19876386

PigR

Regulator	Description	Reference
PigR	Indirect activator	PMID: 19876386

MigR

Regulator	Description	Reference
MigR (FTL_1542)	Indirect activator	PMID: 19349423

FevR

Regulator	Description	Reference
FevR	Activator	PMID: 19349423

MglA

Regulator	Description	Reference
MglA (FTL_1185)	Activator	PMID: 17000729

Hfq

Regulator	Description	Reference
Hfq	Repressor	PMID: 19223477

(1) Lindgren M et al. (2013). Importance of PdpC, IglC, IglI, and IglG for modulation of a host cell death pathway induced by Francisella tularensis LVS. Infect Immun. 81(6):2076-84. [PudMed:23529623]

(2) Lindgren M et al. (2013). The Francisella tularensis LVS DeltapdpC mutant exhibits a unique phenotype during intracellular infection. BMC Microbiol. 13(1):20. [PudMed:23356941]

(3) Broms JE et al. (2012). Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631]

(4) Broms JE et al. (2012). DotU and VgrG, core components of type VI secretion systems, are essential for Francisella LVS pathogenicity. PLoS One. 7(4):e34639. [PudMed:22514651]

(5) de Bruin OM et al. (2011). The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115]

(6) Broms JE et al. (2011). IglG and IglI of the Francisella pathogenicity island are important virulence determinants of Francisella tularensis LVS. Infect Immun. 79(9):3683-96. [PudMed:21690239]

(7) Nano FE et al. (2007). The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]

This literature contains experimental investigation