I. Summary

SecReT6 ID	467
Strain	Francisella tularensis subsp. holarctica LVS
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_007880
Location	1096796..1118939
Comment	Growth, phagosomal escape and cytopathogenicity in cultured macrophages; virulence in mouse model
Function	anti-eukaryotic; effector translocation;virulence
T6SS Type	Type ii (Type FPI)
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	PdpA	PdpB	IglE	VgrG	IglF	IglG	IglH	DotU	IglI
Number	1	1	1	1	1	1	1	1	1
Component	IglJ	PdpC	PdpE	IglD	IglC	IglB	IglA	PdpD
Number	1	1	1	1	1	1	1	2

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	FTL_RS05850	1091858..1092922 [+], 1065	489106285	fructose-bisphosphate aldolase class II
2	FTL_RS10420	1093090..1094543 [-], 1454	pseudogene	alpha,alpha-trehalase
3	FTL_RS05860	1094545..1094802 [-], 258	489106287	hypothetical protein
4	FTL_RS10425	1094950..1095830 [+], 881	499776592	IS630 family transposase
5	FTL_RS05875	1095831..1096703 [-], 873	499776609	MFS transporter
6	FTL_RS10895	1096688..1097410 [+], 723	pseudogene	Pathogenicity determinant protein D
7	FTL_RS05890 (tssB)	1097491..1098045 [+], 555	489106300	type VI secretion system contractile sheath small subunit
8	FTL_RS05895 (tssC)	1098059..1099579 [+], 1521	497717462	type VI secretion system contractile sheath large subunit
9	FTL_RS05900	1099596..1100225 [+], 630	489106304	hypothetical protein
10	FTL_RS05905	1100246..1101442 [+], 1197	499776611	hypothetical protein
11	FTL_RS05910	1101449..1102024 [-], 576	489106308	hypothetical protein
12	FTL_RS05915 (pdpC)	1102042..1106028 [-], 3987	489106311	pathogenicity determinant protein PdpC
13	FTL_RS05920	1106068..1106841 [-], 774	489106314	hypothetical protein
14	FTL_RS05925	1106807..1107958 [-], 1152	489106318	hypothetical protein
15	FTL_RS05930	1107980..1108603 [-], 624	489106320	DotU family type IV/VI secretion system protein
16	FTL_RS05935	1108616..1110046 [-], 1431	489106321	hypothetical protein
17	FTL_RS05940	1110059..1110580 [-], 522	489106324	DUF4280 domain-containing protein
18	FTL_RS05945	1110648..1112312 [-], 1665	489106327	hypothetical protein
19	FTL_RS05950	1112317..1112811 [-], 495	489106331	hypothetical protein
20	FTL_RS05955	1112824..1113201 [-], 378	489108377	hypothetical protein
21	FTL_RS05960	1113191..1116472 [-], 3282	499776612	hypothetical protein
22	FTL_RS05965	1116477..1118939 [-], 2463	489106334	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

FTL_0118 (IglI) , FTT_1347 (VgrG)

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

N/A

Regulatory element identified

SspA

Regulator	Description	Reference
SspA (FTL_1606)	Indirect activator	PMID: 19876386

PigR

Regulator	Description	Reference
PigR	Indirect activator	PMID: 19876386

MigR

Regulator	Description	Reference
MigR (FTL_1542)	Indirect activator	PMID: 19349423

FevR

Regulator	Description	Reference
FevR	Activator	PMID: 19349423

MglA

Regulator	Description	Reference
MglA (FTL_1185)	Activator	PMID: 17000729

Hfq

Regulator	Description	Reference
Hfq	Repressor	PMID: 19223477

(1) Lindgren M et al. (2013). Importance of PdpC, IglC, IglI, and IglG for modulation of a host cell death pathway induced by Francisella tularensis LVS. Infect Immun. 81(6):2076-84. [PudMed:23529623]

(2) Lindgren M et al. (2013). The Francisella tularensis LVS DeltapdpC mutant exhibits a unique phenotype during intracellular infection. BMC Microbiol. 13(1):20. [PudMed:23356941]

(3) Broms JE et al. (2012). Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631]

(4) Broms JE et al. (2012). DotU and VgrG, core components of type VI secretion systems, are essential for Francisella LVS pathogenicity. PLoS One. 7(4):e34639. [PudMed:22514651]

(5) de Bruin OM et al. (2011). The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115]

(6) Broms JE et al. (2011). IglG and IglI of the Francisella pathogenicity island are important virulence determinants of Francisella tularensis LVS. Infect Immun. 79(9):3683-96. [PudMed:21690239]

(7) Nano FE et al. (2007). The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]

This literature contains experimental investigation