SecReT6
I. Summary
SecReT6 ID467 experimental
StrainFrancisella tularensis subsp. holarctica LVS
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_007880
Location1096796..1118939
CommentGrowth, phagosomal escape and cytopathogenicity in cultured macrophages; virulence in mouse model
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType ii (Type FPI)
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentPdpAPdpBIglEVgrGIglFIglGIglHDotUIglI
Number111111111
ComponentIglJPdpCPdpEIglDIglCIglBIglAPdpD
Number11111112
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1FTL_11491091858..1092922 [+], 106589256473fructose-1,6-bisphosphate aldolase 
2FTL_11501093090..1093644 [-], 55589256474Alpha,alpha-trehalase. 
3FTL_11511093524..1094570 [-], 104789256475trehalase, pseuodgene 
4FTL_1153 (isftu1)1094950..1095830 [+], 88189256476transposase 
5FTL_11551096796..1097197 [+], 40289256477hypothetical protein  PdpD
6FTL_11561097207..1097410 [+], 20489256478hypothetical protein  PdpD
7FTL_11571097491..1098045 [+], 55589256479intracellular growth locus, subunit A  IglA
8FTL_11581098059..1099579 [+], 152189256480intracellular growth locus, subunit B  IglB
9FTL_11591099596..1100225 [+], 63089256481intracellular growth locus, subunit C  IglC
10FTL_11601100246..1101442 [+], 119789256482intracellular growth locus, subunit D  IglD
11FTL_11611101449..1102024 [-], 57689256483hypothetical protein  PdpE
12FTL_11621102042..1106028 [-], 398789256484hypothetical protein  PdpC
13FTL_11631106068..1106841 [-], 77489256485hypothetical protein  IglJ
14FTL_11641106807..1107958 [-], 115289256486hypothetical protein  IglI
15FTL_11651107980..1108603 [-], 62489256487hypothetical protein  DotU
16FTL_11661108616..1110046 [-], 143189256488hypothetical protein  IglH
17FTL_11671110059..1110580 [-], 52289256489hypothetical protein  IglG
18FTL_11681110648..1112312 [-], 166589256490hypothetical protein  IglF
19FTL_11691112317..1112811 [-], 49589256491hypothetical protein  VgrG
20FTL_11701112824..1113201 [-], 37889256492hypothetical protein  IglE
21FTL_11711113191..1116472 [-], 328289256493hypothetical protein  PdpB
22FTL_11721116477..1118939 [-], 246389256494hypothetical protein  PdpA
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
FTL_0118 (IglI) , FTT_1347 (VgrG)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1FTL_11641106807..1107958 [-], 115289256486hypothetical protein (IglI)FTL_0118 (IglI)
2FTL_11691112317..1112811 [-], 49589256491hypothetical protein (VgrG)FTT_1347 (VgrG)
Data from experimental literature

Download FASTA format files
Proteins        Genes
N/A
Regulatory element identified
SspA
RegulatorDescriptionReference
SspA (FTL_1606)Indirect activatorPMID: 19876386

PigR
RegulatorDescriptionReference
PigRIndirect activatorPMID: 19876386

MigR
RegulatorDescriptionReference
MigR (FTL_1542)Indirect activatorPMID: 19349423

FevR
RegulatorDescriptionReference
FevRActivatorPMID: 19349423

MglA
RegulatorDescriptionReference
MglA (FTL_1185)ActivatorPMID: 17000729

Hfq
RegulatorDescriptionReference
HfqRepressorPMID: 19223477

(1) Lindgren M et al. (2013). Importance of PdpC, IglC, IglI, and IglG for modulation of a host cell death pathway induced by Francisella tularensis LVS. Infect Immun. 81(6):2076-84. [PudMed:23529623] experimental
(2) Lindgren M et al. (2013). The Francisella tularensis LVS DeltapdpC mutant exhibits a unique phenotype during intracellular infection. BMC Microbiol. 13(1):20. [PudMed:23356941] experimental
(3) Broms JE et al. (2012). Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631] experimental
(4) Broms JE et al. (2012). DotU and VgrG, core components of type VI secretion systems, are essential for Francisella LVS pathogenicity. PLoS One. 7(4):e34639. [PudMed:22514651] experimental
(5) de Bruin OM et al. (2011). The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115] experimental
(6) Broms JE et al. (2011). IglG and IglI of the Francisella pathogenicity island are important virulence determinants of Francisella tularensis LVS. Infect Immun. 79(9):3683-96. [PudMed:21690239] experimental
(7) Nano FE et al. (2007). The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]
 
experimental This literature contains experimental investigation