SecReT6
I. Summary
SecReT6 ID467 experimental
StrainFrancisella tularensis subsp. holarctica LVS
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_007880
Location1096796..1118939
CommentGrowth, phagosomal escape and cytopathogenicity in cultured macrophages; virulence in mouse model
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType ii (Type FPI)
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentPdpAPdpBIglEVgrGIglFIglGIglHDotUIglI
Number111111111
ComponentIglJPdpCPdpEIglDIglCIglBIglAPdpD
Number11111112
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1FTL_RS058501091858..1092922 [+], 1065489106285fructose-bisphosphate aldolase class II 
2FTL_RS104201093090..1094543 [-], 1454pseudogenealpha,alpha-trehalase 
3FTL_RS058601094545..1094802 [-], 258489106287hypothetical protein 
4FTL_RS104251094950..1095830 [+], 881499776592IS630 family transposase 
5FTL_RS058751095831..1096703 [-], 873499776609MFS transporter 
6FTL_RS108951096688..1097410 [+], 723pseudogenePathogenicity determinant protein D 
7FTL_RS05890 (tssB)1097491..1098045 [+], 555489106300type VI secretion system contractile sheath small subunit 
8FTL_RS05895 (tssC)1098059..1099579 [+], 1521497717462type VI secretion system contractile sheath large subunit 
9FTL_RS059001099596..1100225 [+], 630489106304hypothetical protein 
10FTL_RS059051100246..1101442 [+], 1197499776611hypothetical protein 
11FTL_RS059101101449..1102024 [-], 576489106308hypothetical protein 
12FTL_RS05915 (pdpC)1102042..1106028 [-], 3987489106311pathogenicity determinant protein PdpC 
13FTL_RS059201106068..1106841 [-], 774489106314hypothetical protein 
14FTL_RS059251106807..1107958 [-], 1152489106318hypothetical protein 
15FTL_RS059301107980..1108603 [-], 624489106320DotU family type IV/VI secretion system protein 
16FTL_RS059351108616..1110046 [-], 1431489106321hypothetical protein 
17FTL_RS059401110059..1110580 [-], 522489106324DUF4280 domain-containing protein 
18FTL_RS059451110648..1112312 [-], 1665489106327hypothetical protein 
19FTL_RS059501112317..1112811 [-], 495489106331hypothetical protein 
20FTL_RS059551112824..1113201 [-], 378489108377hypothetical protein 
21FTL_RS059601113191..1116472 [-], 3282499776612hypothetical protein 
22FTL_RS059651116477..1118939 [-], 2463489106334hypothetical protein 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
FTL_0118 (IglI) , FTT_1347 (VgrG)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes
N/A
Regulatory element identified
SspA
RegulatorDescriptionReference
SspA (FTL_1606)Indirect activatorPMID: 19876386

PigR
RegulatorDescriptionReference
PigRIndirect activatorPMID: 19876386

MigR
RegulatorDescriptionReference
MigR (FTL_1542)Indirect activatorPMID: 19349423

FevR
RegulatorDescriptionReference
FevRActivatorPMID: 19349423

MglA
RegulatorDescriptionReference
MglA (FTL_1185)ActivatorPMID: 17000729

Hfq
RegulatorDescriptionReference
HfqRepressorPMID: 19223477

(1) Lindgren M et al. (2013). Importance of PdpC, IglC, IglI, and IglG for modulation of a host cell death pathway induced by Francisella tularensis LVS. Infect Immun. 81(6):2076-84. [PudMed:23529623] experimental
(2) Lindgren M et al. (2013). The Francisella tularensis LVS DeltapdpC mutant exhibits a unique phenotype during intracellular infection. BMC Microbiol. 13(1):20. [PudMed:23356941] experimental
(3) Broms JE et al. (2012). Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631] experimental
(4) Broms JE et al. (2012). DotU and VgrG, core components of type VI secretion systems, are essential for Francisella LVS pathogenicity. PLoS One. 7(4):e34639. [PudMed:22514651] experimental
(5) de Bruin OM et al. (2011). The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115] experimental
(6) Broms JE et al. (2011). IglG and IglI of the Francisella pathogenicity island are important virulence determinants of Francisella tularensis LVS. Infect Immun. 79(9):3683-96. [PudMed:21690239] experimental
(7) Nano FE et al. (2007). The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]
 
experimental This literature contains experimental investigation