I. Summary
SecReT6 ID5 experimental
StrainPseudomonas aeruginosa PAO1
Repliconchromosome (H3-T6SS) [Browse all T6SS(s) in this replicon]
CommentH3-T6SS is required for virulence in the worm model; PldB exerts an antibacterial activity and facilitates eukaryotic cells by activation of the PI3K/Akt pathway; PldB contributes to the internalization of P. aeruginosa into Human Epithelial cells.
Functionanti-eukaryotic and anti-bacterial; effector translocation; virulence
T6SS TypeType i; subtype i4a
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1PA23492595985..2596779 [+], 79515597545hypothetical protein 
2PA23502596776..2597885 [+], 111015597546methionine ABC transporter ATP-binding protein 
3PA23512597869..2598522 [+], 65415597547ABC transporter permease 
4PA23522598700..2599827 [+], 112815597548glycerophosphoryl diester phosphodiesterase 
5PA23532599893..2601047 [-], 115515597549hypothetical protein 
6PA23542601219..2602349 [-], 113115597550transcriptional regulator 
7PA23552602346..2603530 [-], 118515597551FMNH2-dependent monooxygenase 
8PA2356 (msuD)2603560..2604705 [-], 114615597552methanesulfonate monooxygenase 
9PA2357 (msuE)2604715..2605275 [-], 56115597553FMN reductase MsuE 
10PA23582605435..2605824 [-], 39015597554hypothetical protein 
11PA23592605937..2607022 [-], 108615597555transcriptional regulator  Sfa3
12PA23602607132..2608232 [-], 110115597556hypothetical protein  TssA
13PA23612608229..2612044 [-], 381615597557hypothetical protein  TssM
14PA23622612041..2612799 [-], 75915597558hypothetical protein  TssL
15PA23632612817..2614148 [-], 133215597559hypothetical protein  TssK
16PA23642614208..2614684 [-], 47715597560hypothetical protein 
17PA23652614893..2615438 [+], 54615597561hypothetical protein  TssB
18PA23662615461..2616945 [+], 148515597562uricase  TssC
19PA23672617019..2617516 [+], 49815597563hypothetical protein  TssD
20PA23682617529..2617954 [+], 42615597564hypothetical protein  TssE
21PA23692617938..2619731 [+], 179415597565hypothetical protein  TssF
22PA23702619695..2620711 [+], 101715597566hypothetical protein  TssG
23PA23712620713..2623262 [+], 255015597567ClpA/B-type protease  TssH
24PA23732624204..2626210 [+], 200715597569hypothetical protein  TssI
25PA23742626221..2626757 [+], 53715597570hypothetical protein 
26PA23752626780..2627175 [-], 39615597571hypothetical protein 
27PA23762627452..2628093 [+], 64215597572transcriptional regulator 
28PA23772628225..2629499 [+], 127515597573hypothetical protein 
29PA23782629916..2632231 [-], 231615597574aldehyde dehydrogenase 
30PA23792632228..2632698 [-], 47115597575oxidoreductase 
31PA23802632963..2633199 [-], 23715597576hypothetical protein 
32PA23812633494..2633736 [+], 24315597577hypothetical protein 
33PA2382 (lldA)2633803..2634954 [-], 115215597578L-lactate dehydrogenase 
34PA23832635051..2635971 [-], 92115597579transcriptional regulator 
35PA23842636013..2636336 [-], 32415597580hypothetical protein 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
PA2374 , PA3907, PA5089 (PldB)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA23742626221..2626757 [+], 53715597570hypothetical protein PA2374
2PA39074375230..4376015 [+], 78615599102hypothetical protein PA3907
3PA50895727239..5729476 [-], 223815600282hypothetical protein (PldB)PA5089 (PldB)
Data from experimental literature

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Proteins        Genes

Homology Immunity protein list
PA3908, PA5086, PA5087, PA5088

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA39084376012..4376731 [+], 72015599103hypothetical protein PA3908
3PA50875725479..5726348 [-], 87015600280hypothetical protein PA5087
4PA50885726357..5727238 [-], 88215600281hypothetical protein PA5088
Data from experimental literature

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Proteins        Genes
Regulatory element identified
PqsE (PA1000)ActivatorPMID: 15686549

LasR (PA1430)ActivatorPMID: 19497948

MvfR (PA1003)ActivatorPMID: 15686549

MvaT (PA4315)RepressorPMID: 19028873

RpoN Sigma 54 factor (PA4462)Repressor: right operonPMID: 24204589
RpoN Sigma 54 factor (PA4462)Activator: left operonPMID: 24204589

RhlI (PA3476)ActivatorPMID: 24204589
RhlR (PA3477)ActivatorPMID: 24204589

Adaptors list
PA3905, PA3906

Adaptor identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA39054374330..4374857 [+], 52815599100hypothetical protein PA3905
2PA39064374850..4375233 [+], 38415599101hypothetical protein PA3906
Data from experimental literature

Download FASTA format files
Proteins        Genes
(1) Chen Z et al. (2014). Cloning, purification, crystallization and preliminary X-ray studies of the putative type VI secretion immunity protein Tli5 (PA5088) from Pseudomonas aeruginosa. Acta Crystallogr F Struct Biol Commun. 70(Pt 7):903-5. [PudMed:25005085] experimental in_silico
(2) Jiang F et al. (2014). A Pseudomonas aeruginosa Type VI Secretion Phospholipase D Effector Targets Both Prokaryotic and Eukaryotic Cells. Cell Host Microbe. 15(5):600-10. [PudMed:24832454] experimental
(3) Sana TG et al. (2013). Divergent Control of Two Type VI Secretion Systems by RpoN in Pseudomonas aeruginosa. PLoS One. 8(10):e76030. [PudMed:24204589] experimental
(4) Kefala K et al. (2012). Purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal fragment of the MvfR protein from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68(Pt 6):695-7. [PudMed:22684073]
(5) Osipiuk J et al. (2011). Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa. J Struct Funct Genomics. 12(1):21-6. [PudMed:21476004]
(6) Barret M et al. (2011). Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] in_silico
(7) Hachani A et al. (2011). Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 286(14):12317-27. [PudMed:21325275] experimental
(8) Bleves S et al. (2010). Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol. 300(8):534-43. [PudMed:20947426]
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation