I. Summary

SecReT6 ID	513
Strain	Vibrio cholerae O1 str. 2010EL-1786
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_016446
Location	889678..913466
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	0	1	1	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

13

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	Vch1786_II0899	885523..887235 [-], 1713	360038109	ribosomal large subunit pseudouridine synthase A
2	Vch1786_II0900	888203..889246 [+], 1044	360038110	hypothetical protein
3	Vch1786_II0901	889678..890184 [+], 507	360038111	type VI secretion system protein ImpB	TssB
4	Vch1786_II0902	890225..891703 [+], 1479	360038112	type VI secretion system protein ImpC	TssC
5	Vch1786_II0903	891706..892143 [+], 438	360038113	type VI secretion system protein	TssE
6	Vch1786_II0904	892149..893918 [+], 1770	360038114	type VI secretion system protein ImpG	TssF
7	Vch1786_II0905	893882..894898 [+], 1017	360038115	type VI secretion system protein ImpH	TssG
8	Vch1786_II0906	894901..896388 [+], 1488	360038116	type VI secretion system protein ImpI	Fha
9	Vch1786_II0907	896391..896867 [+], 477	360038117	type VI secretion system protein VasD	TssJ
10	Vch1786_II0908	896874..898208 [+], 1335	360038118	type VI secretion system protein ImpJ	TssK
11	Vch1786_II0909	898211..898984 [+], 774	360038119	type VI secretion system protein ImpK	TssL
12	Vch1786_II0910	899010..901619 [+], 2610	360038120	type VI secretion system protein VasG	TssH
13	Vch1786_II0911	901622..903214 [+], 1593	360038121	sigma-54 dependent transcriptional regulator	VasH
14	Vch1786_II0912	903211..903867 [+], 657	360038122	type VI secretion system protein VasI	VCA0118
15	Vch1786_II0913	903877..905286 [+], 1410	360038123	type VI secretion system protein VasJ	TssA
16	Vch1786_II0914	905302..908847 [+], 3546	360038124	type VI secretion system protein ImpL	TssM
17	Vch1786_II0915	908898..910160 [+], 1263	360038125	type VI secretion system protein VasL	TssA
18	Vch1786_II0916	910413..913466 [+], 3054	360038126	VgrG protein	TssI
19	Vch1786_II0917	913463..913831 [+], 369	360038127	hypothetical protein	TsiV3 (i)
20	Vch1786_II0919	913979..914239 [-], 261	360038129	hypothetical protein
21	Vch1786_II0918	914214..914423 [+], 210	360038128	hypothetical protein
22	Vch1786_II0920 (rbsD)	914523..914942 [+], 420	360038130	D-ribose pyranase
23	Vch1786_II0921 (rbsA)	914969..916471 [+], 1503	360038131	D-ribose transporter ATP binding protein
24	Vch1786_II0922 (rbsC)	916486..917469 [+], 984	360038132	ribose ABC transporter permease protein
25	Vch1786_II0923 (rbsB)	917530..918408 [+], 879	360038133	D-ribose transporter subunit RbsB

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

VCA0017 , VC1416, VC1418, VCA0285, VCA0017, VCA0018, VCA0020, VCA0118, VCA0123

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	Vch1786_I0914(hcp)	986816..987145 [+], 330	360035309	hcp protein	VCA0017
2	Vch1786_I0915	987273..990764 [+], 3492	360035310	type VI secretion system secreted protein VgrG	VC1416
3	Vch1786_I0917	991606..993531 [+], 1926	360035312	hypothetical protein	VC1418
4	Vch1786_II0031	31645..32316 [+], 672	360037245	hypothetical protein	VCA0285
5	Vch1786_II0813(hcp-1)	792614..793132 [+], 519	360038023	hcp protein	VCA0017
6	Vch1786_II0814	793260..795344 [+], 2085	360038024	type VI secretion system secreted protein VgrG	VCA0018
7	Vch1786_II0816	796228..799485 [+], 3258	360038026	hypothetical protein	VCA0020
8	Vch1786_II0912	903211..903867 [+], 657	360038122	type VI secretion system protein VasI	VCA0118
9	Vch1786_II0916	910413..913466 [+], 3054	360038126	VgrG protein	VCA0123

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Proteins        Genes

Homology Immunity protein list

VC1419, VCA0286, VCA0021, VCA0124

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	Vch1786_I0918	993528..994271 [+], 744	360035313	hypothetical protein	VC1419
2	Vch1786_II0032	32318..32668 [+], 351	360037246	hypothetical protein	VCA0286
3	Vch1786_II0817	799486..800214 [+], 729	360038027	hypothetical protein	VCA0021
4	Vch1786_II0917	913463..913831 [+], 369	360038127	hypothetical protein	VCA0124

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Proteins        Genes

N/A