I. Summary

SecReT6 ID	536
Strain	Pantoea ananatis LMG 5342
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_016816
Location	1820000..1860045
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

14

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	PANA5342_1719 (amn)	1815004..1816458 [-], 1455	378766686	AMP nucleosidase
2	PANA5342_1720 (yeeO)	1816849..1818267 [+], 1419	378766687	MATE family transport protein
3	PANA5342_1721	1819377..1819976 [+], 600	378766688	type VI secretion system-associated hypothetical protein
4	PANA5342_1722	1820000..1820503 [+], 504	378766689	type VI secretion system, core protein	TssJ
5	PANA5342_1723	1820525..1821865 [+], 1341	378766690	type VI secretion system, core protein	TssK
6	PANA5342_1724	1821882..1823123 [+], 1242	378766691	type IV/VI secretion system protein, DotU family	TssL
7	PANA5342_1725 (icmF1)	1823127..1826753 [+], 3627	378766692	type VI secretion protein IcmF	TssM
8	PANA5342_1726	1826768..1827481 [+], 714	378766693	type VI secretion system-associated hypothetical protein
9	PANA5342_1727 (impA)	1827492..1828514 [+], 1023	378766694	type VI secretion-associated protein, ImpA family	TssA
10	PANA5342_1728	1828595..1829125 [+], 531	378766695	type VI secretion system, core protein	TssB
11	PANA5342_1729	1829125..1830624 [+], 1500	378766696	type VI secretion system, core protein	TssC
12	PANA5342_1730 (hcp5)	1831205..1831687 [+], 483	378766697	type VI secretion system effector	TssD
13	PANA5342_1731	1831757..1832614 [+], 858	378766698	peptidoglycan-binding domain 1 protein
14	PANA5342_1732	1833289..1834149 [+], 861	378766699	hypothetical protein
15	PANA5342_1733	1834746..1835297 [+], 552	378766700	hypothetical protein
16	PANA5342_1734	1835392..1835793 [+], 402	378766701	type VI secretion system-associated hypothetical protein
17	PANA5342_1735 (fha1)	1835927..1837822 [+], 1896	378766702	FHA domain-containing protein	Fha
18	PANA5342_1736 (pppA1)	1837819..1838610 [+], 792	378766703	type VI secretion system, serine/threonine protein phosphatase
19	PANA5342_1737	1838579..1839634 [+], 1056	378766704	type VI secretion system-associated hypothetical protein
20	PANA5342_1738	1839651..1840478 [+], 828	378766705	type VI secretion system-associated hypothetical protein	TagJ
21	PANA5342_1739	1840468..1841043 [+], 576	378766706	type VI secretion system lysozyme-like protein	TssE
22	PANA5342_1740	1841046..1842923 [+], 1878	378766707	type VI secretion system, core protein	TssF
23	PANA5342_1741	1842920..1843969 [+], 1050	378766708	type VI secretion system, core protein	TssG
24	PANA5342_1742 (clpV)	1844023..1846632 [+], 2610	378766709	type VI secretion ATPase, ClpV1 family	TssH
25	PANA5342_1743 (ppkA1)	1846662..1848125 [+], 1464	378766710	serine/threonine-protein kinase PpkA
26	PANA5342_1744 (vgrG1)	1848137..1850362 [+], 2226	378766711	ImpA family type VI secretion-associated protein	TssI
27	PANA5342_1745	1852659..1855199 [+], 2541	378766712	hypothetical protein
28	PANA5342_1746	1855196..1856266 [+], 1071	378766713	pentapeptide repeat-containing protein
29	PANA5342_1747	1856279..1856884 [+], 606	378766714	hypothetical protein
30	PANA5342_1748	1856894..1857286 [+], 393	378766715	hypothetical protein
31	PANA5342_1749 (vgrG3)	1857334..1860045 [+], 2712	378766716	ImpA family type VI secretion-associated protein	TssI
32	PANA5342_1750	1860059..1860619 [+], 561	378766717	hypothetical protein
33	PANA5342_1751	1862637..1863383 [+], 747	378766718	hypothetical protein
34	PANA5342_1752	1863394..1864242 [+], 849	378766719	hypothetical protein
35	PANA5342_1753	1864255..1864695 [+], 441	378766720	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

SMA2263 , SMA3456, SMA2263, ECL_01567, ECL_03140

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PANA5342_1639(hcp1)	1736924..1737406 [+], 483	378766607	type VI secretion system effector	SMA2263
2	PANA5342_1640(hcp3)	1737437..1737919 [+], 483	378766608	type VI secretion system effector	SMA3456
3	PANA5342_1730(hcp5)	1831205..1831687 [+], 483	378766697	type VI secretion system effector	SMA2263
4	PANA5342_1754(rhsD1)	1864699..1869153 [+], 4455	378766721	RHS Repeat family protein	ECL_01567
5	PANA5342_3230(rhsD7)	3373950..3378110 [+], 4161	378768189	Rhs family protein	ECL_03140

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Proteins        Genes

N/A

N/A