I. Summary

SecReT6 ID	543
Strain	Salmonella enterica subsp. enterica serovar Typhi str. P-stx-12
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_016832
Location	2647188..2693724
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	2	1	1	1	2	1	0	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	0

13

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	STBHUCCB_27220	2642635..2646699 [-], 4065	378960750	Rhs-family protein	PAAR
2	STBHUCCB_27230	2646718..2647164 [-], 447	378960751	hypothetical protein
3	STBHUCCB_27240	2647188..2649377 [-], 2190	378960752	Rhs-family protein	TssI
4	STBHUCCB_27250	2649855..2650292 [-], 438	378960753	pathogenicity island protein
5	STBHUCCB_27260	2650319..2650705 [-], 387	378960754	hypothetical protein
6	STBHUCCB_27270	2650732..2650854 [-], 123	378960755	hypothetical protein
7	STBHUCCB_27280	2650863..2651324 [-], 462	378960756	hypothetical protein
8	STBHUCCB_27290	2651883..2652356 [-], 474	378960757	hypothetical protein
9	STBHUCCB_27300	2652341..2652811 [-], 471	378960758	hypothetical protein
10	STBHUCCB_27310	2652854..2653621 [-], 768	378960759	hypothetical protein
11	STBHUCCB_27340	2657719..2658213 [-], 495	378960760	hypothetical protein
12	STBHUCCB_27350	2658245..2658994 [-], 750	378960761	hypothetical protein
13	STBHUCCB_27360	2658999..2660300 [-], 1302	378960762	hypothetical protein	TssL
14	STBHUCCB_27370	2660297..2661640 [-], 1344	378960763	Type VI secretion protein	TssK
15	STBHUCCB_27380	2661644..2662135 [-], 492	378960764	Type VI secretion lipoprotein	TssJ
16	STBHUCCB_27390	2662247..2662732 [-], 486	378960765	hypothetical protein	TssD
17	STBHUCCB_27400	2662969..2663394 [-], 426	378960766	hypothetical protein	STY0301 (i)
18	STBHUCCB_27430	2665352..2665894 [-], 543	378960767	Type VI secretion protein	TssB
19	STBHUCCB_27440	2665958..2666248 [-], 291	378960768	hypothetical protein
20	STBHUCCB_27450	2666334..2668997 [-], 2664	378960769	hypothetical protein	TssH
21	STBHUCCB_27460	2669377..2670267 [+], 891	378960770	hypothetical protein
22	STBHUCCB_27470	2670254..2671078 [+], 825	378960771	hypothetical protein	TagJ
23	STBHUCCB_27480	2671075..2671569 [+], 495	378960772	Type VI secretion system lysozyme-related protein	TssE
24	STBHUCCB_27490	2671585..2673468 [+], 1884	378960773	Type VI secretion protein	TssF
25	STBHUCCB_27500	2673465..2674460 [+], 996	378960774	hypothetical protein	TssG
26	STBHUCCB_27510	2674477..2674893 [+], 417	378960775	ImpA-related N-family protein	TssA
27	STBHUCCB_27520	2674893..2675525 [+], 633	378960776	ImpA-related N-family protein	TssA
28	STBHUCCB_27530	2676055..2676795 [-], 741	378960777	DNA polymerase III subunit epsilon
29	STBHUCCB_27540	2676850..2677317 [+], 468	378960778	ribonuclease H
30	STBHUCCB_27550	2677314..2678036 [-], 723	378960779	hypothetical protein
31	STBHUCCB_27560	2678071..2678826 [+], 756	378960780	hydroxyacylglutathione hydrolase
32	STBHUCCB_27570	2679045..2680265 [+], 1221	378960781	membrane-bound lytic murein transglycosylase D
33	STBHUCCB_27580	2680321..2681091 [-], 771	378960782	S-adenosyl-L-methionine-dependent methyltransferase
34	STBHUCCB_27590	2681169..2681969 [-], 801	378960783	hypothetical protein
35	STBHUCCB_27600	2682101..2683276 [-], 1176	378960784	major facilitator family transporter
36	STBHUCCB_27610	2683381..2684295 [+], 915	378960785	LysR family transcriptional regulator
37	STBHUCCB_27620	2684316..2685119 [-], 804	378960786	2,5-diketo-D-gluconic acid reductase B
38	STBHUCCB_27630	2691151..2693724 [-], 2574	378960787	hypothetical protein	TssH
39	STBHUCCB_27640	2693854..2694585 [-], 732	378960788	hypothetical protein
40	STBHUCCB_27650	2694582..2695562 [-], 981	378960789	ribosomal large subunit pseudouridine synthase D
41	STBHUCCB_27660	2695694..2696431 [+], 738	378960790	hypothetical protein
42	STBHUCCB_27670	2696703..2697041 [+], 339	378960791	ribosome-associated inhibitor A
43	STBHUCCB_27680	2697292..2698452 [+], 1161	378960792	prephenate dehydratase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

STY2606 , SciK, Hcp

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	STBHUCCB_5270	562364..562732 [-], 369	378958634	hypothetical protein	STY2606
2	STBHUCCB_27390	2662247..2662732 [-], 486	378960765	hypothetical protein	SciK
3	STBHUCCB_32180	3120957..3121442 [-], 486	378961225	hypothetical protein	Hcp

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Proteins        Genes

Homology Immunity protein list

STY2607, STY0301

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	STBHUCCB_5260	561846..562364 [-], 519	378958633	hypothetical protein	STY2607
2	STBHUCCB_27400	2662969..2663394 [-], 426	378960766	hypothetical protein	STY0301

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Proteins        Genes

N/A