I. Summary

SecReT6 ID	61
Strain	Acinetobacter sp. ADP1
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_005966
Location	2633143..2661510
Comment
Function	effector translocation
T6SS Type	Type i; subtype i4b
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	0	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	ACIAD2674	2628937..2629482 [-], 546	50085741	electron transfer flavoprotein
2	ACIAD2675	2629502..2630743 [-], 1242	50085742	oxygenase subunit protein
3	ACIAD2676	2630795..2631646 [-], 852	50085743	short chain dehydrogenase
4	ACIAD2677	2631616..2632899 [-], 1284	50085744	dienelactone hydrolase
5	ACIAD2678	2633143..2634144 [-], 1002	50085745	transcriptional regulator
6	ACIAD2680 (gdh)	2634401..2635672 [-], 1272	50085746	glutamate dehydrogenase (NAD(P)+) oxidoreductase protein
7	ACIAD2681	2635901..2636167 [-], 267	50085747	hypothetical protein	PAAR
8	ACIAD2682	2636197..2636961 [-], 765	50085748	outer membrane lipoprotein	TagL
9	ACIAD2683	2636964..2637923 [-], 960	50085749	hypothetical protein
10	ACIAD2684	2637964..2641785 [-], 3822	50085750	hypothetical protein	TssM
11	ACIAD2685	2641820..2643250 [-], 1431	50085751	hypothetical protein
12	ACIAD2686	2643220..2644218 [-], 999	50085752	hypothetical protein	TssG
13	ACIAD2687	2644182..2645990 [-], 1809	50085753	hypothetical protein	TssF
14	ACIAD2688	2646002..2646475 [-], 474	50085754	hypothetical protein	TssE
15	ACIAD2689	2646545..2647048 [-], 504	50085755	hypothetical protein	TssD
16	ACIAD2690	2647094..2648590 [-], 1497	50085756	hypothetical protein	TssC
17	ACIAD2691	2648568..2649077 [-], 510	50085757	hypothetical protein	TssB
18	ACIAD2693	2649091..2649747 [-], 657	50085758	hypothetical protein
19	ACIAD2694	2650125..2652809 [+], 2685	50085759	ClpA/B-type chaperone	TssH
20	ACIAD2695	2652825..2653928 [+], 1104	50085760	hypothetical protein	TssA
21	ACIAD2696	2653945..2655312 [+], 1368	50085761	hypothetical protein	TssK
22	ACIAD2697	2655324..2656127 [+], 804	50085762	hypothetical protein	TssL
23	ACIAD2698	2656138..2656719 [+], 582	50085763	hypothetical protein
24	ACIAD2699	2656820..2657683 [+], 864	50085764	hypothetical protein
25	ACIAD2700	2657701..2658882 [-], 1182	50085765	hypothetical protein
26	ACIAD2701	2659669..2660025 [+], 357	50085766	hypothetical protein
27	ACIAD2702	2660030..2660530 [+], 501	50085767	hypothetical protein
28	ACIAD2704	2660893..2661510 [+], 618	50085768	hypothetical protein
29	ACIAD2705	2661512..2661904 [+], 393	50085769	hypothetical protein
30	ACIAD2707	2662166..2662330 [+], 165	50085770	hypothetical protein
31	ACIAD2708	2662359..2663201 [+], 843	50085771	hypothetical protein
32	ACIAD2709	2663226..2663423 [+], 198	50085772	hypothetical protein
33	ACIAD2710	2663420..2663560 [+], 141	50085773	hypothetical protein
34	ACIAD2711	2663658..2664314 [+], 657	50085774	hypothetical protein
35	ACIAD2712	2664311..2664601 [+], 291	50085775	hypothetical protein
36	ACIAD2713	2664856..2665167 [+], 312	50085776	hypothetical protein
37	ACIAD2714	2665176..2665442 [+], 267	50085777	hypothetical protein
38	ACIAD2715	2665640..2665873 [+], 234	50085778	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

ACIAD0053 , ACIAD0168, A1S_1296, ACIAD3114, ACIAD3425, ACIAD1790–1794 fusion

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	ACIAD0053	55985..56617 [+], 633	50083347	zinc protease	ACIAD0053
2	ACIAD0168	170197..172380 [+], 2184	50083453	hypothetical protein	ACIAD0168
3	ACIAD2689	2646545..2647048 [-], 504	50085755	hypothetical protein	A1S_1296
4	ACIAD3114	3040392..3043397 [-], 3006	50086118	hypothetical protein	ACIAD3114
5	ACIAD3425	3339596..3341326 [-], 1731	50086393	hypothetical protein	ACIAD3425
Data from experimental literature

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Proteins        Genes

Homology Immunity protein list

ACIAD0054, ACIAD0169, ACIAD1795, ACIAD1796, ACIAD3112, ACIAD3113, ACIAD3426

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	ACIAD0054	56618..57070 [+], 453	50083348	hypothetical protein	ACIAD0054
2	ACIAD0169	172382..173107 [+], 726	50083454	hypothetical protein	ACIAD0169
3	ACIAD1795	1802446..1803534 [+], 1089	50084943	hypothetical protein	ACIAD1795
4	ACIAD1796	1803547..1804635 [+], 1089	50084944	hypothetical protein	ACIAD1796
5	ACIAD3112	3038267..3039250 [+], 984	50086116	hypothetical protein	ACIAD3112
6	ACIAD3113	3039389..3040372 [+], 984	50086117	hypothetical protein	ACIAD3113
7	ACIAD3426	3341328..3342386 [-], 1059	50086394	hypothetical protein	ACIAD3426
Data from experimental literature

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Proteins        Genes

Adaptors list

ACIAD2685, TagX

Adaptor identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	ACIAD2685	2641820..2643250 [-], 1431	50085751	hypothetical protein	ACIAD2685
Data from experimental literature

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Proteins        Genes

(1) Shneider MM et al. (2013). PAAR-repeat proteins sharpen and diversify the type VI secretion system spike. Nature. 500(7462):350-3. [PudMed:23925114]

(2) Basler M et al. (2013). Tit-for-Tat: Type VI Secretion System Counterattack during Bacterial Cell-Cell Interactions. Cell. 152(4):884-94. [PudMed:23415234]

(3) Weber BS et al. (2013). Genomic and Functional Analysis of the Type VI Secretion System in Acinetobacter. PLoS One. 8(1):e55142. [PudMed:23365692]

(4) Boyer F et al. (2009). Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 10:104. [PudMed:19284603]

This literature contains experimental investigation

This literature contains bioinformatics investigation