I. Summary

SecReT6 ID	611
Strain	Pseudomonas aeruginosa M18
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_017548
Location	89946..115973
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

14

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	PAM18_0077 (pppA)	88531..89259 [-], 729	386056146	PppA	PppA
2	PAM18_0078	89269..89949 [-], 681	386056147	hypothetical protein	TagF
3	PAM18_0079 (icmF1)	89946..93476 [-], 3531	386056148	IcmF1	TssM
4	PAM18_0080	93473..94822 [-], 1350	386056149	hypothetical protein	TssL
5	PAM18_0081	94829..96163 [-], 1335	386056150	hypothetical protein	TssK
6	PAM18_0082	96179..96643 [-], 465	386056151	hypothetical protein	TssJ
7	PAM18_0083 (fha1)	96688..98205 [-], 1518	386056152	Fha1	Fha
8	PAM18_0084	98573..99607 [+], 1035	386056153	hypothetical protein	TssA
9	PAM18_0085	99696..100214 [+], 519	386056154	hypothetical protein	TssB
10	PAM18_0086	100227..101723 [+], 1497	386056155	hypothetical protein	TssC
11	PAM18_0087 (hcp1)	101811..102287 [+], 477	386056156	hypothetical protein	TssD
12	PAM18_0088	102515..103300 [+], 786	386056157	hypothetical protein	TagJ
13	PAM18_0089	103302..103811 [+], 510	386056158	hypothetical protein	TssE
14	PAM18_0090	103808..105667 [+], 1860	386056159	hypothetical protein	TssF
15	PAM18_0091	105631..106677 [+], 1047	386056160	hypothetical protein	TssG
16	PAM18_0092 (clpV1)	106670..109378 [+], 2709	386056161	ClpV1 protein	TssH
17	PAM18_0093 (vgrG1)	109416..111356 [+], 1941	386056162	VgrG1	TssI
18	PAM18_0094	111471..111755 [-], 285	386056163	hypothetical protein	Tsi6 (i)
19	PAM18_0095	111752..113044 [-], 1293	386056164	hypothetical protein	Tse6 (e)
20	PAM18_0096	113060..113494 [-], 435	386056165	hypothetical protein
21	PAM18_0097	113748..115973 [+], 2226	386056166	hypothetical protein	TssI
22	PAM18_0098	116001..116450 [+], 450	386056167	hypothetical protein
23	PAM18_0099	116467..117579 [+], 1113	386056168	hypothetical protein
24	PAM18_0100	117576..118613 [+], 1038	386056169	3-oxoacyl-(acyl carrier protein) synthase
25	PAM18_0101	118613..119773 [+], 1161	386056170	hypothetical protein	PA0099 (e)
26	PAM18_0102	119795..120715 [+], 921	386056171	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PA0085 , PA0091, PA0093, PA0095, PA0099, PA3484, PA2774, PA2702, PA2685, PA2684, PA1844

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PAM18_0087(hcp1)	101811..102287 [+], 477	386056156	hypothetical protein	PA0085
2	PAM18_0093(vgrG1)	109416..111356 [+], 1941	386056162	VgrG1	PA0091
3	PAM18_0095	111752..113044 [-], 1293	386056164	hypothetical protein	PA0093
4	PAM18_0097	113748..115973 [+], 2226	386056166	hypothetical protein	PA0095
5	PAM18_0101	118613..119773 [+], 1161	386056170	hypothetical protein	PA0099
6	PAM18_1481	1629184..1630410 [-], 1227	386057548	hypothetical protein	PA3484
7	PAM18_2189	2386674..2387261 [+], 588	386058252	hypothetical protein	PA2774
8	PAM18_2270	2461803..2462279 [-], 477	386058333	hypothetical protein	PA2702
9	PAM18_2287	2481311..2483380 [+], 2070	386058350	hypothetical protein	PA2685
10	PAM18_2288	2483430..2487383 [+], 3954	386058351	hypothetical protein	PA2684
11	PAM18_3198	3575434..3575898 [+], 465	386059261	hypothetical protein	PA1844

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Proteins        Genes

Homology Immunity protein list

PA0092, PA3485, PA2775, PA2703, Tsi5, PA1845

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PAM18_0094	111471..111755 [-], 285	386056163	hypothetical protein	PA0092
2	PAM18_1480	1628750..1629187 [-], 438	386057547	hypothetical protein	PA3485
3	PAM18_2188	2386232..2386669 [+], 438	386058251	hypothetical protein	PA2775
4	PAM18_2269	2461560..2461793 [-], 234	386058332	hypothetical protein	PA2703
5	PAM18_2289	2487380..2487571 [+], 192	386058352	hypothetical protein	Tsi5
6	PAM18_3197	3574874..3575392 [+], 519	386059260	hypothetical protein	PA1845

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Proteins        Genes

Regulatory element identified

GacA

Regulator	Description	Reference
GacA (PAM18_2407)	Activator	PMID: 23708134

(1) Wei X et al. (2013). Global control of GacA on secondary metabolism, primary metabolism, secretion systems, and motility in the rhizobacterium Pseudomonas aeruginosa M18. J Bacteriol. 195(15):3387-400. [PudMed:23708134]

This literature contains experimental investigation