I. Summary

SecReT6 ID	613
Strain	Pseudomonas aeruginosa M18
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_017548
Location	3757284..3776259
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	0	1	0	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	PAM18_3371	3752833..3753153 [+], 321	386059434	hypothetical protein
2	PAM18_3372	3753210..3753767 [+], 558	386059435	hypothetical protein
3	PAM18_3373 (folE)	3753845..3754390 [+], 546	386059436	GTP cyclohydrolase I
4	PAM18_3374	3754455..3754916 [-], 462	386059437	hypothetical protein
5	PAM18_3375	3755162..3755542 [+], 381	386059438	hypothetical protein
6	PAM18_3376 (stk1)	3755570..3756559 [-], 990	386059439	serine-threonine kinase Stk1	Stk1
7	PAM18_3377 (stp1)	3756556..3757284 [-], 729	386059440	serine/threonine phosphoprotein phosphatase Stp1	Stp1
8	PAM18_3378	3757284..3760811 [-], 3528	386059441	hypothetical protein	TssM
9	PAM18_3379	3760827..3761696 [-], 870	386059442	putative outer membrane protein	TssL
10	PAM18_3380	3761699..3763030 [-], 1332	386059443	hypothetical protein	TssK
11	PAM18_3381	3763027..3763542 [-], 516	386059444	putative lipoprotein	TssJ
12	PAM18_3382	3763539..3764732 [-], 1194	386059445	hypothetical protein	Fha
13	PAM18_3383	3764979..3766490 [-], 1512	386059446	putative transcriptional regulator	Sfa2
14	PAM18_3384	3766501..3769128 [-], 2628	386059447	putative ClpA/B-type protease	TssH
15	PAM18_3385	3769141..3770148 [-], 1008	386059448	hypothetical protein	TssG
16	PAM18_3386	3770112..3771899 [-], 1788	386059449	hypothetical protein	TssF
17	PAM18_3387	3772237..3772644 [-], 408	386059450	hypothetical protein	TssE
18	PAM18_3388	3772657..3774132 [-], 1476	386059451	hypothetical protein	TssC
19	PAM18_3389	3774161..3774667 [-], 507	386059452	hypothetical protein	TssB
20	PAM18_3390	3774703..3776259 [-], 1557	386059453	hypothetical protein	TssA
21	PAM18_3391	3777431..3778033 [-], 603	386059454	putative glutathione S-transferase
22	PAM18_3392	3778089..3779255 [-], 1167	386059455	putative aminotransferase
23	PAM18_3393	3779337..3779813 [+], 477	386059456	putative transcriptional regulator
24	PAM18_3394	3779863..3780498 [-], 636	386059457	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PSPTO_5435 , PSPTO_5435, PSPTO_5435

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PAM18_0258(hcpB)	289792..290310 [-], 519	386056327	secreted protein Hcp	PSPTO_5435
2	PAM18_3619(hcpB)	4022380..4022898 [+], 519	386059680	secreted protein Hcp	PSPTO_5435
3	PAM18_5387(hcpB)	5987719..5988237 [-], 519	386061444	secreted protein Hcp	PSPTO_5435

Download FASTA format files

Proteins        Genes

N/A

Regulatory element identified

GacA

Regulator	Description	Reference
GacA (PAM18_2407)	Activator	PMID: 23708134

(1) Wei X et al. (2013). Global control of GacA on secondary metabolism, primary metabolism, secretion systems, and motility in the rhizobacterium Pseudomonas aeruginosa M18. J Bacteriol. 195(15):3387-400. [PudMed:23708134]

This literature contains experimental investigation