I. Summary

SecReT6 ID	614
Strain	Pseudomonas aeruginosa NCGM2.S1
Replicon	chromosome I [Browse all T6SS(s) in this replicon]
Accession	NC_017549
Location	95453..121818
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	NCGM2_0078 (ppkA)	90932..94030 [-], 3099	386063051	serine/threonine protein kinase	PpkA
2	NCGM2_0079	94038..94766 [-], 729	386063052	putative phosphoprotein phosphatase	PppA
3	NCGM2_0080	94776..95456 [-], 681	386063053	hypothetical protein	TagF
4	NCGM2_0081	95453..98986 [-], 3534	386063054	hypothetical protein	TssM
5	NCGM2_0082	98983..100332 [-], 1350	386063055	hypothetical protein	TssL
6	NCGM2_0083	100339..101673 [-], 1335	386063056	hypothetical protein	TssK
7	NCGM2_0084	101689..102153 [-], 465	386063057	putative lipoprotein	TssJ
8	NCGM2_0085	102198..103721 [-], 1524	386063058	hypothetical protein	Fha
9	NCGM2_0086	104089..105123 [+], 1035	386063059	hypothetical protein	TssA
10	NCGM2_0087	105212..105730 [+], 519	386063060	hypothetical protein	TssB
11	NCGM2_0088	105743..107239 [+], 1497	386063061	hypothetical protein	TssC
12	NCGM2_0089	107315..107803 [+], 489	386063062	hypothetical protein	TssD
13	NCGM2_0090	108031..108816 [+], 786	386063063	putative secretion protein	TagJ
14	NCGM2_0091	108818..109327 [+], 510	386063064	hypothetical protein	TssE
15	NCGM2_0092	109324..111183 [+], 1860	386063065	hypothetical protein	TssF
16	NCGM2_0093	111147..112193 [+], 1047	386063066	hypothetical protein	TssG
17	NCGM2_0094 (clpB)	112186..114894 [+], 2709	386063067	putative ClpA/B-type chaperone	TssH
18	NCGM2_0095	114932..116872 [+], 1941	386063068	hypothetical protein	TssI
19	NCGM2_0096	116987..117262 [-], 276	386063069	hypothetical protein	Tsi6 (i)
20	NCGM2_0097	117284..117517 [-], 234	386063070	hypothetical protein
21	NCGM2_0098	117507..118889 [-], 1383	386063071	hypothetical protein	Tse6 (e)
22	NCGM2_0099	118905..119339 [-], 435	386063072	hypothetical protein
23	NCGM2_0100	119593..121818 [+], 2226	386063073	hypothetical protein	TssI
24	NCGM2_0101	121846..122295 [+], 450	386063074	hypothetical protein
25	NCGM2_0102	122312..123424 [+], 1113	386063075	hypothetical protein
26	NCGM2_0103	123421..124458 [+], 1038	386063076	3-oxoacyl-(acyl carrier protein) synthase
27	NCGM2_0104	124458..125618 [+], 1161	386063077	hypothetical protein	PA0099 (e)
28	NCGM2_0105	125640..126560 [+], 921	386063078	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PA0085 , PA0091, PA0093, PA0095, PA0099, PA1844, PA2684, PA2685, PA2702, PA2774, PA3484

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	NCGM2_0089	107315..107803 [+], 489	386063062	hypothetical protein	PA0085
2	NCGM2_0095	114932..116872 [+], 1941	386063068	hypothetical protein	PA0091
3	NCGM2_0098	117507..118889 [-], 1383	386063071	hypothetical protein	PA0093
4	NCGM2_0100	119593..121818 [+], 2226	386063073	hypothetical protein	PA0095
5	NCGM2_0104	124458..125618 [+], 1161	386063077	hypothetical protein	PA0099
6	NCGM2_2759	2935844..2936308 [-], 465	386065692	hypothetical protein	PA1844
7	NCGM2_3711(rhs)	4048405..4052358 [-], 3954	386066636	hypothetical protein	PA2684
8	NCGM2_3712	4052408..4054477 [-], 2070	386066637	hypothetical protein	PA2685
9	NCGM2_3729	4073509..4073985 [+], 477	386066654	hypothetical protein	PA2702
10	NCGM2_3822	4166027..4166614 [-], 588	386066745	hypothetical protein	PA2774
11	NCGM2_4624	4979572..4980798 [+], 1227	386067528	hypothetical protein	PA3484

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Proteins        Genes

Homology Immunity protein list

PA0092, PA1845, PA2703, PA2775, PA3485

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	NCGM2_0096	116987..117262 [-], 276	386063069	hypothetical protein	PA0092
2	NCGM2_2760	2936350..2936868 [-], 519	386065693	hypothetical protein	PA1845
3	NCGM2_3730	4073995..4074228 [+], 234	386066655	hypothetical protein	PA2703
4	NCGM2_3823	4166619..4167056 [-], 438	386066746	hypothetical protein	PA2775
5	NCGM2_4625	4980795..4981232 [+], 438	386067529	hypothetical protein	PA3485

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Proteins        Genes

N/A