I. Summary

SecReT6 ID	615
Strain	Pseudomonas aeruginosa NCGM2.S1
Replicon	chromosome I [Browse all T6SS(s) in this replicon]
Accession	NC_017549
Location	2732174..2754590
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	NCGM2_2550	2728078..2731440 [-], 3363	386065489	putative Rhs family protein	RhsP2 (e)
2	NCGM2_2551	2731437..2731841 [-], 405	386065490	hypothetical protein
3	NCGM2_2552	2732174..2734210 [-], 2037	386065491	hypothetical protein	TssI
4	NCGM2_2553 (hcpD)	2734406..2734924 [-], 519	386065492	secreted protein	TssD
5	NCGM2_2554	2735622..2737178 [+], 1557	386065493	hypothetical protein	TssA
6	NCGM2_2555	2737214..2737720 [+], 507	386065494	hypothetical protein	TssB
7	NCGM2_2556	2737748..2739223 [+], 1476	386065495	hypothetical protein	TssC
8	NCGM2_2557	2739236..2739643 [+], 408	386065496	hypothetical protein	TssE
9	NCGM2_2558	2739975..2741762 [+], 1788	386065497	hypothetical protein	TssF
10	NCGM2_2559	2741726..2742733 [+], 1008	386065498	hypothetical protein	TssG
11	NCGM2_2560	2742746..2745373 [+], 2628	386065499	putative ClpA/B-type protease	TssH
12	NCGM2_2561	2745384..2746895 [+], 1512	386065500	putative transcriptional regulator	Sfa2
13	NCGM2_2562	2746984..2747124 [+], 141	386065501	hypothetical protein
14	NCGM2_2563	2747142..2748335 [+], 1194	386065502	hypothetical protein	Fha
15	NCGM2_2564	2748332..2748847 [+], 516	386065503	putative lipoprotein	TssJ
16	NCGM2_2565	2748844..2750175 [+], 1332	386065504	hypothetical protein	TssK
17	NCGM2_2566	2750178..2751047 [+], 870	386065505	hypothetical protein	TssL
18	NCGM2_2567	2751063..2754590 [+], 3528	386065506	hypothetical protein	TssM
19	NCGM2_2568 (stp1)	2754590..2755318 [+], 729	386065507	serine/threonine phosphoprotein phosphatase	Stp1
20	NCGM2_2569 (stk1)	2755315..2756304 [+], 990	386065508	serine-threonine kinase	Stk1
21	NCGM2_2570	2756332..2756661 [-], 330	386065509	hypothetical protein
22	NCGM2_2571	2756958..2757419 [+], 462	386065510	hypothetical protein
23	NCGM2_2572 (folE)	2757484..2758029 [-], 546	386065511	GTP cyclohydrolase I
24	NCGM2_2573	2758107..2758664 [-], 558	386065512	hypothetical protein
25	NCGM2_2574	2758721..2759041 [-], 321	386065513	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PSPTO_5435 , PSPTO_5435, PA14_43100, PSPTO_5435, PA3487, PSPTO_5435

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	NCGM2_0270(hcpD)	296633..297151 [-], 519	386063243	secreted protein	PSPTO_5435
2	NCGM2_2397(hcpD)	2567474..2567992 [-], 519	386065336	secreted protein	PSPTO_5435
3	NCGM2_2550	2728078..2731440 [-], 3363	386065489	putative Rhs family protein	PA14_43100
4	NCGM2_2553(hcpD)	2734406..2734924 [-], 519	386065492	secreted protein	PSPTO_5435
5	NCGM2_4629(pldA)	4983314..4986613 [+], 3300	386067533	phospholipase D	PA3487
6	NCGM2_6030(hcpD)	6424591..6425109 [-], 519	386068921	secreted protein	PSPTO_5435

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Proteins        Genes

Homology Immunity protein list

PA3488

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	NCGM2_4630	4986706..4987740 [+], 1035	386067534	hypothetical protein	PA3488

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Proteins        Genes

N/A