I. Summary

SecReT6 ID	620
Strain	Ralstonia solanacearum Po82
Replicon	megaplasmid [Browse all T6SS(s) in this replicon]
Accession	NC_017575
Location	1199515..1233446
Comment
Function	N/A
T6SS Type	Type i; subtype i4b
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	4	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	RSPO_m01062 (otsA)	1197161..1198369 [+], 1209	386336065	alpha,alpha-trehalose-phosphate synthase (UDP-forming)
2	RSPO_m01063	1198485..1199267 [+], 783	386336066	transmembrane protein
3	RSPO_m01064	1199321..1199494 [-], 174	386336067	transmembrane protein
4	RSPO_m01065	1199515..1200198 [-], 684	386336068	hypothetical protein	TssI
5	RSPO_m01066	1200259..1201047 [-], 789	386336069	hypothetical protein	TssL
6	RSPO_m01067	1201044..1202390 [-], 1347	386336070	hypothetical protein	TssK
7	RSPO_m01068	1202445..1202591 [-], 147	386336071	putative transmembrane protein
8	RSPO_m01069	1203399..1204046 [+], 648	386336072	lipoprotein
9	RSPO_m01070	1204083..1204595 [+], 513	386336073	hypothetical protein	TssB
10	RSPO_m01071	1204588..1206078 [+], 1491	386336074	hypothetical protein	TssC
11	RSPO_m01072 (sciK)	1206167..1206670 [+], 504	386336075	secretion system protein (evpc-like), hemolysin-coregulated protein	TssD
12	RSPO_m01073	1206731..1207204 [+], 474	386336076	hypothetical protein	TssE
13	RSPO_m01074	1207267..1209117 [+], 1851	386336077	hypothetical protein	TssF
14	RSPO_m01075	1209081..1210184 [+], 1104	386336078	hypothetical protein	TssG
15	RSPO_m01076 (clpB)	1210217..1212940 [+], 2724	386336079	chaperone clpb	TssH
16	RSPO_m01077	1212977..1213861 [+], 885	386336080	VgrG protein	TssI
17	RSPO_m01078	1213599..1214219 [+], 621	386336081	vgr-related protein	TssI
18	RSPO_m01079	1214697..1215638 [+], 942	386336082	hypothetical protein
19	RSPO_m01081	1215822..1215974 [+], 153	386336083	hypothetical protein
20	RSPO_m01082	1215990..1217168 [+], 1179	386336084	hypothetical protein
21	RSPO_m01083	1217215..1217691 [+], 477	386336085	hypothetical protein
22	RSPO_m01084	1217952..1219325 [+], 1374	386336086	twin-arginine translocation pathway signal harboring protein
23	RSPO_m01085	1219458..1220537 [+], 1080	386336087	hypothetical protein	TssA
24	RSPO_m01087	1220595..1221437 [-], 843	386336088	peptidoglycan-associated lipoprotein	TagL
25	RSPO_m01086	1221434..1222420 [-], 987	386336089	hypothetical protein
26	RSPO_m01088 (icmF)	1222417..1226352 [-], 3936	386336090	transmembrane protein	TssM
27	RSPO_m01089	1226389..1227885 [-], 1497	386336091	hypothetical protein
28	RSPO_m01090	1227896..1228165 [-], 270	386336092	hypothetical protein	PAAR
29	RSPO_m01091	1228204..1229052 [-], 849	386336093	L-alanyl-D-glutamate peptidase
30	RSPO_m01092	1229338..1230096 [+], 759	386336094	hypothetical protein
31	RSPO_m01093	1230234..1233446 [+], 3213	386336095	vgr-related protein	TssI
32	RSPO_m01094	1233241..1233609 [+], 369	386336096	hypothetical protein
33	RSPO_m01095	1233666..1233947 [+], 282	386336097	hypothetical protein
34	RSPO_m01096	1233982..1234362 [+], 381	386336098	hypothetical protein
35	RSPO_m01097	1234346..1234486 [-], 141	386336099	hypothetical protein
36	RSPO_m01098	1234538..1235047 [-], 510	386336100	hypothetical protein
37	RSPO_m01099	1235335..1235583 [-], 249	386336101	hypothetical protein
38	RSPO_m01100 (aspC)	1235604..1236791 [-], 1188	386336102	aspartate aminotransferase
39	RSPO_m01101 (nac)	1236935..1237822 [-], 888	386336103	nitrogen assimilation transcriptional regulator

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

RSp0745

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	RSPO_m01072(sciK)	1206167..1206670 [+], 504	386336075	secretion system protein (evpc-like), hemolysin-coregulated protein	RSp0745

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Proteins        Genes

N/A

N/A