I. Summary

SecReT6 ID	626
Strain	Escherichia coli UM146
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_017632
Location	3334311..3373707
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	2	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

14

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	UM146_16035 (prfH)	3330037..3330651 [-], 615	386605778	peptide chain release factor-like protein
2	UM146_16040	3330648..3331787 [-], 1140	386605779	hypothetical protein
3	UM146_16045	3331977..3332429 [-], 453	386605780	hypothetical protein
4	UM146_16050	3332439..3332837 [-], 399	386605781	putative toxin YafO
5	UM146_16055	3332840..3333133 [-], 294	386605782	putative antitoxin of the YafO-YafN toxin-antitoxin system
6	UM146_16060	3333185..3334240 [-], 1056	386605783	DNA polymerase IV
7	UM146_16065	3334311..3335081 [-], 771	386605784	hypothetical protein	TagL
8	UM146_16070	3335041..3336780 [+], 1740	386605785	flagellar system protein
9	UM146_16075	3337099..3337596 [-], 498	386605786	hypothetical protein
10	UM146_16080	3337772..3338530 [-], 759	386605787	putative exported hydrolase
11	UM146_16085	3338833..3339573 [+], 741	386605788	hypothetical protein
12	UM146_16090	3339544..3340311 [-], 768	386605789	putative glutamine amidotransferases class-II
13	UM146_16095 (gmhA)	3340516..3341094 [-], 579	386605790	phosphoheptose isomerase
14	UM146_16100 (fadE)	3341334..3343778 [+], 2445	386605791	acyl-CoA dehydrogenase
15	UM146_16105	3343821..3344294 [-], 474	386605792	C-lysozyme inhibitor
16	UM146_16110	3344448..3345218 [+], 771	386605793	hypothetical protein
17	UM146_16115	3345489..3346001 [-], 513	386605794	hypothetical protein
18	UM146_16120	3346071..3346574 [-], 504	386605795	hypothetical protein
19	UM146_16125	3346803..3347288 [-], 486	386605796	putative transposase
20	UM146_16130	3347388..3347567 [-], 180	386605797	putative H-repeat associated protein
21	UM146_16135	3347825..3348115 [-], 291	386605798	hypothetical protein
22	UM146_16140	3348090..3349154 [-], 1065	386605799	hypothetical protein
23	UM146_16145	3349522..3350568 [-], 1047	386605800	hypothetical protein
24	UM146_16150	3350675..3352657 [-], 1983	386605801	hypothetical protein	TssI
25	UM146_16155	3352673..3352783 [-], 111	386605802	hypothetical protein
26	UM146_16160	3352878..3353396 [-], 519	386605803	hypothetical protein	TssD
27	UM146_16165	3354102..3354605 [+], 504	386605804	type VI secretion protein	TssB
28	UM146_16170	3354628..3356112 [+], 1485	386605805	type VI secretion protein EvpB	TssC
29	UM146_16175	3356117..3356542 [+], 426	386605806	type VI secretion system lysozyme-like protein	TssE
30	UM146_16180	3356548..3358389 [+], 1842	386605807	hypothetical protein	TssF
31	UM146_16185	3358353..3359402 [+], 1050	386605808	hypothetical protein	TssG
32	UM146_16190	3359407..3360699 [+], 1293	386605809	hypothetical protein	Fha
33	UM146_16195	3360696..3361220 [+], 525	386605810	hypothetical protein	TssJ
34	UM146_16200	3361223..3362569 [+], 1347	386605811	type VI secretion protein	TssK
35	UM146_16205	3362574..3363338 [+], 765	386605812	hypothetical protein	TssL
36	UM146_16210	3363348..3366107 [+], 2760	386605813	putative ATPase with chaperone activity	TssH
37	UM146_16215	3366104..3366847 [+], 744	386605814	type VI secretion-associated protein
38	UM146_16220	3366852..3368294 [+], 1443	386605815	hypothetical protein	TssA
39	UM146_16225	3368314..3371841 [+], 3528	386605816	hypothetical protein	TssM
40	UM146_16240	3373228..3373707 [+], 480	386605817	hypothetical protein	TssD
41	UM146_16245	3374047..3374832 [-], 786	386605818	putative aminopeptidase
42	UM146_16250	3375370..3376110 [-], 741	386605819	DNA polymerase III subunit epsilon
43	UM146_16255 (rnhA)	3376166..3376633 [+], 468	386605820	ribonuclease H
44	UM146_16260	3376630..3377352 [-], 723	386605821	putative methyltransferase
45	UM146_16265	3377386..3378141 [+], 756	386605822	hydroxyacylglutathione hydrolase
46	UM146_16270 (mltD)	3378213..3378581 [+], 369	386605823	membrane-bound lytic murein transglycosylase D

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

ABG57132

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	UM146_16160	3352878..3353396 [-], 519	386605803	hypothetical protein	ABG57132

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Proteins        Genes

N/A

N/A