I. Summary

SecReT6 ID	633
Strain	Escherichia coli str. 'clone D i2'
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_017651
Location	3111538..3137828
Comment
Function	N/A
T6SS Type	Type i; subtype i2
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

14

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	i02_3103	3106703..3107944 [+], 1242	386630550	cysteine sulfinate desulfinase
2	i02_3104 (ygdK)	3107944..3108387 [+], 444	386630551	hypothetical protein
3	i02_3105 (ygdL)	3108438..3109244 [-], 807	386630552	hypothetical protein
4	i02_3106 (mltA)	3109321..3110418 [-], 1098	386630553	murein transglycosylase A
5	i02_3107	3111538..3112059 [+], 522	386630554	hypothetical protein	TssB
6	i02_3108	3112112..3113656 [+], 1545	386630555	hypothetical protein	TssC
7	i02_3109	3113676..3115013 [+], 1338	386630556	hypothetical protein	TssK
8	i02_3110	3115010..3115675 [+], 666	386630557	hypothetical protein	TssL
9	i02_3111	3115688..3117340 [+], 1653	386630558	hypothetical protein	TagL
10	i02_3112	3117398..3117889 [+], 492	386630559	secreted protein Hcp	TssD
11	i02_3113	3118081..3120717 [+], 2637	386630560	ClpB protein	TssH
12	i02_3114	3120717..3123191 [+], 2475	386630561	hypothetical protein	TssI
13	i02_3115	3123206..3124030 [+], 825	386630562	hypothetical protein
14	i02_3116	3124027..3126003 [+], 1977	386630563	hypothetical protein
15	i02_3117	3126013..3126285 [+], 273	386630564	hypothetical protein	PAAR
16	i02_3118	3126285..3127718 [+], 1434	386630565	hypothetical protein
17	i02_3119	3127890..3131063 [+], 3174	386630566	hypothetical protein	TssM
18	i02_3120	3131029..3132666 [+], 1638	386630567	hypothetical protein	TssA
19	i02_3121	3132700..3134460 [+], 1761	386630568	hypothetical protein	TssF
20	i02_3122	3134424..3135503 [+], 1080	386630569	hypothetical protein	TssG
21	i02_3123	3135484..3136020 [+], 537	386630570	hypothetical protein	TssJ
22	i02_3124	3136024..3136452 [+], 429	386630571	hypothetical protein	TssE
23	i02_3125	3136452..3137828 [+], 1377	386630572	hypothetical protein	TssA
24	i02_3127	3138128..3139084 [-], 957	386630573	2-hydroxyacid dehydrogenase
25	i02_3128	3139147..3139743 [-], 597	386630574	phosphosugar isomerase
26	i02_3129	3139746..3140921 [-], 1176	386630575	Beta-cystathionase
27	i02_3130	3140921..3142501 [-], 1581	386630576	PTS system, maltose and glucose-specific IIABC

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

EC042_4529

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	i02_3112	3117398..3117889 [+], 492	386630559	secreted protein Hcp	EC042_4529

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Proteins        Genes

N/A

N/A