I. Summary

SecReT6 ID	640
Strain	Providencia stuartii MRSN 2154
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_017731
Location	2033995..2058046
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	2	1	1	1	2
Component	TssG	TssJ	TssK	TssM
Number	1	1	2	2

17

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	S70_09485	2029256..2029552 [-], 297	386743269	hypothetical protein	PAAR
2	S70_09490	2029603..2030493 [-], 891	386743270	hypothetical protein
3	S70_09495	2030730..2030987 [-], 258	386743271	hypothetical protein	PAAR
4	S70_09500	2031057..2031467 [-], 411	386743272	hypothetical protein
5	S70_09505	2031561..2033195 [-], 1635	386743273	hypothetical protein
6	S70_09510	2033192..2033998 [-], 807	386743274	hypothetical protein
7	S70_09515	2033995..2036142 [-], 2148	386743275	VgrG-like protein	TssI
8	S70_09520	2036191..2037927 [-], 1737	386743276	hypothetical protein	TssF
9	S70_09525	2037947..2038426 [-], 480	386743277	Hcp-like protein	TssD
10	S70_09530	2038439..2039770 [-], 1332	386743278	hypothetical protein	TssA
11	S70_09535	2039811..2042579 [-], 2769	386743279	hypothetical protein	TssM
12	S70_09540	2042555..2043358 [-], 804	386743280	hypothetical protein	TssM
13	S70_09545	2043355..2044818 [-], 1464	386743281	hypothetical protein	TssA
14	S70_09550	2044824..2045468 [-], 645	386743282	hypothetical protein
15	S70_09555	2045465..2046262 [-], 798	386743283	hypothetical protein
16	S70_09560	2046259..2048874 [-], 2616	386743284	ATPase	TssH
17	S70_09565	2048883..2049638 [-], 756	386743285	putative membrane protein Aec26	TssL
18	S70_09570	2049631..2050785 [-], 1155	386743286	hypothetical protein	TssK
19	S70_09575	2050835..2050990 [-], 156	386743287	hypothetical protein	TssK
20	S70_09580	2051042..2051545 [-], 504	386743288	lipoprotein	TssJ
21	S70_09585	2051547..2052818 [-], 1272	386743289	phosphopeptide-binding protein	Fha
22	S70_09590	2052824..2053861 [-], 1038	386743290	hypothetical protein	TssG
23	S70_09595	2053825..2055606 [-], 1782	386743291	hypothetical protein	TssF
24	S70_09600	2055608..2056045 [-], 438	386743292	hypothetical protein	TssE
25	S70_09605	2056050..2057528 [-], 1479	386743293	hypothetical protein	TssC
26	S70_09610	2057549..2058046 [-], 498	386743294	hypothetical protein	TssB
27	S70_09615	2059186..2061495 [-], 2310	386743295	glucan 1,4-alpha-glucosidase
28	S70_09620	2061492..2062934 [-], 1443	386743296	glucose-6-phosphate 1-dehydrogenase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

idrB , ABG57132, ABG57132, idrB, ABG57132, ECA2866

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	S70_11165	2396923..2399079 [-], 2157	386743596	Rhs family protein	idrB
2	S70_11170	2399163..2399681 [-], 519	386743597	hypothetical protein	ABG57132
3	S70_13845	2948907..2949425 [-], 519	386744116	hypothetical protein	ABG57132
4	S70_14750	3128043..3130199 [-], 2157	386744289	Rhs family protein	idrB
5	S70_14755	3130283..3130801 [-], 519	386744290	hypothetical protein	ABG57132
6	S70_18075	3801783..3802301 [-], 519	386744933	hypothetical protein	ECA2866

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Proteins        Genes

N/A

N/A