I. Summary

SecReT6 ID	642
Strain	Burkholderia pseudomallei 1026b
Replicon	chromosome I [Browse all T6SS(s) in this replicon]
Accession	NC_017831
Location	174161..194147
Comment
Function	N/A
T6SS Type	Type i; subtype i4b
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	0	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

12

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BP1026B_I0176	170479..170598 [-], 120	386860296	hypothetical protein
2	BP1026B_I0177	171372..171698 [+], 327	386860297	phage baseplate assembly protein
3	BP1026B_I0178	171691..172104 [+], 414	386860298	phage tail protein I
4	BP1026B_I0179	172122..172604 [+], 483	386860299	Phage-encoded modification methylase
5	BP1026B_I0180	173084..173590 [+], 507	386860300	hypothetical protein
6	BP1026B_I0181	173694..174059 [-], 366	386860301	phage tail completion protein
7	BP1026B_I0182 (tssL-1)	174161..174946 [-], 786	386860302	type VI secretion system	TssL
8	BP1026B_I0183 (tssK-1)	174943..176289 [-], 1347	386860303	type VI secretion system	TssK
9	BP1026B_I0184 (tssJ-1)	176398..177051 [-], 654	386860304	type VI secretion system	TssJ
10	BP1026B_I0185	177020..177124 [-], 105	386860305	hypothetical protein
11	BP1026B_I0186 (tagM-1)	177387..178058 [+], 672	386860306	lipoprotein
12	BP1026B_I0187 (tssB-1)	178095..178613 [+], 519	386860307	type VI secretion system	TssB
13	BP1026B_I0188 (tssC-1)	178630..180120 [+], 1491	386860308	type VI secretion system	TssC
14	BP1026B_I0189 (hcp-1)	180175..180696 [+], 522	386860309	type VI secretion system	TssD
15	BP1026B_I0190 (tssE-1)	180754..181236 [+], 483	386860310	type VI secretion system	TssE
16	BP1026B_I0191 (tssF-1)	181316..183154 [+], 1839	386860311	type VI secretion system	TssF
17	BP1026B_I0192 (tssG-1)	183118..184218 [+], 1101	386860312	type VI secretion system	TssG
18	BP1026B_I0193 (clpV-1)	184252..186921 [+], 2670	386860313	type VI secretion system	TssH
19	BP1026B_I0194 (tssA-1)	187011..188132 [+], 1122	386860314	type VI secretion system	TssA
20	BP1026B_I0195 (tagN-1)	188331..189263 [-], 933	386860315	type VI secretion system	TagL
21	BP1026B_I0196 (tagF-1)	189268..190257 [-], 990	386860316	type VI secretion system
22	BP1026B_I0197 (tssM-1)	190254..194147 [-], 3894	386860317	type VI secretion system	TssM
23	BP1026B_I0198	194419..194946 [-], 528	386860318	hypothetical protein
24	BP1026B_I0199	195396..196364 [+], 969	386860319	hypothetical protein
25	BP1026B_I0200	196574..197989 [-], 1416	386860320	TolC family type I secretion outer membrane protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

BTH_I0068 , BCAL0343, BTH_I2693, BTH_I2697, BTH_I2698, BTH_I2705, BTH_I3225, BTH_I3226, BTH_I3226, BTH_I3225, BTH_II0310

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BP1026B_I0069	75957..76397 [+], 441	386860195	hypothetical protein	BTH_I0068
2	BP1026B_I0189(hcp-1)	180175..180696 [+], 522	386860309	type VI secretion system	BCAL0343
3	BP1026B_I2019	2253248..2256088 [-], 2841	386862084	Rhs element Vgr protein	BTH_I2693
4	BP1026B_I2025	2258225..2261542 [-], 3318	386862090	hypothetical protein	BTH_I2697
5	BP1026B_I2026	2261669..2263210 [-], 1542	386862091	hypothetical protein	BTH_I2698
6	BP1026B_I2031	2266194..2268992 [-], 2799	386862096	Rhs element Vgr protein	BTH_I2705
7	BP1026B_II0082	88329..91115 [+], 2787	386863816	Rhs element Vgr protein	BTH_I3225
8	BP1026B_II0083	91129..93369 [+], 2241	386863817	hypothetical protein	BTH_I3226
9	BP1026B_II1282	1604949..1607189 [-], 2241	386864979	hypothetical protein	BTH_I3226
10	BP1026B_II1283	1607203..1609989 [-], 2787	386864980	Rhs element Vgr protein	BTH_I3225
11	BP1026B_II2176	2701489..2702394 [+], 906	386865848	lectin	BTH_II0310

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Proteins        Genes

Homology Immunity protein list

BTH_I0069, BTH_I2700

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BP1026B_I0070	76384..76752 [+], 369	386860196	lipoprotein	BTH_I0069
2	BP1026B_I2029	2264664..2264951 [-], 288	386862094	hypothetical protein	BTH_I2700

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Proteins        Genes

N/A