I. Summary

SecReT6 ID	647
Strain	Burkholderia pseudomallei 1026b
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_017832
Location	2005585..2034286
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BP1026B_II1582	2000759..2001784 [+], 1026	386865270	N-acetylmuramoyl-L-alanine amidase domain-containing protein
2	BP1026B_II1583	2001912..2003312 [+], 1401	386865271	hypothetical protein
3	BP1026B_II1584	2003440..2004885 [+], 1446	386865272	intracellular motility protein A
4	BP1026B_II1585	2004965..2005342 [+], 378	386865273	hypothetical protein
5	BP1026B_II1586 (tssA-5)	2005585..2007483 [-], 1899	386865274	type VI secretion system	TssA
6	BP1026B_II1587	2007474..2008157 [-], 684	386865275	DNA-binding response regulator	VirG
7	BP1026B_II1589	2008208..2010037 [-], 1830	386865276	sensor histidine kinase	VirA
8	BP1026B_II1590 (tssB-5)	2010297..2010791 [+], 495	386865277	type VI secretion system	TssB
9	BP1026B_II1588 (tssC-5)	2010815..2012314 [+], 1500	386865278	type VI secretion system	TssC
10	BP1026B_II1591 (hcp-5)	2012534..2013043 [+], 510	386865279	type VI secretion system	TssD
11	BP1026B_II1592 (tssE-5)	2013036..2013497 [+], 462	386865280	type VI secretion system	TssE
12	BP1026B_II1594 (tssF-5)	2013534..2015276 [+], 1743	386865281	type VI secretion system	TssF
13	BP1026B_II1593 (tssG-5)	2015264..2016286 [+], 1023	386865282	type VI secretion system	TssG
14	BP1026B_II1595 (clpV-5)	2016273..2019356 [+], 3084	386865283	type VI secretion system	TssH
15	BP1026B_II1596 (vgrG-5)	2019383..2022406 [+], 3024	386865284	type VI secretion system	TssI
16	BP1026B_II1597 (tagAB-5)	2022432..2025074 [+], 2643	386865285	type VI secretion system
17	BP1026B_II1598 (tagB-5)	2025092..2026156 [+], 1065	386865286	type VI secretion system
18	BP1026B_II1599 (tagC-5)	2026153..2026905 [+], 753	386865287	type VI secretion system
19	BP1026B_II1600 (tagD-5)	2026934..2027326 [+], 393	386865288	type VI secretion system
20	BP1026B_II1602 (tssJ-5)	2027525..2028133 [+], 609	386865289	type VI secretion system	TssJ
21	BP1026B_II1601 (tssK-5)	2028166..2029563 [+], 1398	386865290	type VI secretion system	TssK
22	BP1026B_II1603 (tssL-5)	2029560..2030225 [+], 666	386865291	type VI secretion system	TssL
23	BP1026B_II1604 (tssM-5)	2030240..2034286 [+], 4047	386865292	type VI secretion system	TssM
24	BP1026B_II1605	2034995..2036218 [+], 1224	386865293	hypothetical protein	BMAA0729 (e)
25	BP1026B_II1606	2036382..2036675 [+], 294	386865294	hypothetical protein
26	BP1026B_II1608	2036626..2037090 [-], 465	386865295	hypothetical protein
27	BP1026B_II1607	2036680..2037312 [+], 633	386865296	GTP cyclohydrolase I
28	BP1026B_II1609	2037746..2037901 [+], 156	386865297	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

BMAA0742 , BMAA0737, BMAA0729

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BP1026B_II1591(hcp-5)	2012534..2013043 [+], 510	386865279	type VI secretion system	BMAA0742
2	BP1026B_II1596(vgrG-5)	2019383..2022406 [+], 3024	386865284	type VI secretion system	BMAA0737
3	BP1026B_II1605	2034995..2036218 [+], 1224	386865293	hypothetical protein	BMAA0729

Download FASTA format files

Proteins        Genes

N/A

(1) Toesca IJ et al. (2014). The Type VI Secretion System Spike Protein VgrG5 Mediates Membrane Fusion during Intercellular Spread by Pseudomallei Group Burkholderia Species. Infect Immun. 82(4):1436-44. [PudMed:24421040]

This literature contains experimental investigation