I. Summary

SecReT6 ID	667
Strain	Pseudomonas putida ND6
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_017986
Location	150200..187494
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

13

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	YSA_00285	145583..147349 [+], 1767	395443789	iron-sulfur cluster-binding protein
2	YSA_00286	147476..147868 [+], 393	395443790	acetyltransferase
3	YSA_00289	147915..148427 [-], 513	395443791	AmiS/UreI transporter
4	YSA_00291	148440..149024 [-], 585	395443792	response regulator receiver/ANTAR domain-containing protein
5	YSA_00292	149021..150181 [-], 1161	395443793	branched-chain amino acid ABC transporter periplasmic component-like protein
6	YSA_00294	150200..151321 [-], 1122	395443794	ATPase	TssH
7	YSA_00295	151377..152420 [-], 1044	395443795	acylamide amidohydrolase
8	YSA_00296	152412..152546 [+], 135	395443796	hypothetical protein
9	YSA_00297	152606..153472 [-], 867	395443797	dihydrodipicolinate synthase
10	YSA_00298	153471..153599 [+], 129	395443798	hypothetical protein
11	YSA_00302	153810..157328 [-], 3519	395443799	cellulose synthase subunit BcsC
12	YSA_00304	157316..158434 [-], 1119	395443800	endo-1,4-D-glucanase
13	YSA_00305	158431..160719 [-], 2289	395443801	Cyclic di-GMP-binding protein
14	YSA_00308	160716..163253 [-], 2538	395443802	cellulose synthase catalytic subunit
15	YSA_00309	163336..164043 [-], 708	395443803	cellulose synthase
16	YSA_00312	164040..164258 [-], 219	395443804	hypothetical protein
17	YSA_00313	164255..165868 [-], 1614	395443805	hypothetical protein
18	YSA_00314	165861..166046 [-], 186	395443806	hypothetical protein
19	YSA_00316	166043..167419 [-], 1377	395443807	hypothetical protein
20	YSA_00315	167483..167611 [+], 129	395443808	hypothetical protein
21	YSA_00317	167593..167706 [+], 114	395443809	hypothetical protein
22	YSA_00320	167713..169689 [+], 1977	395443810	ABC transporter ATP-binding protein	TagT
23	YSA_00322	169767..173387 [-], 3621	395443811	ImcF domain-containing protein	TssM
24	YSA_00323	173384..174841 [-], 1458	395443812	ImpA domain-containing protein	TssA
25	YSA_00324	174835..175392 [-], 558	395443813	hypothetical protein
26	YSA_00325	175513..175692 [+], 180	395443814	hypothetical protein
27	YSA_00327	175812..176315 [+], 504	395443815	hypothetical protein	TssB
28	YSA_00331	176333..177823 [+], 1491	395443816	hypothetical protein	TssC
29	YSA_00332	177820..178230 [+], 411	395443817	GPW/gp25 family protein	TssE
30	YSA_00335	178234..180000 [+], 1767	395443818	hypothetical protein	TssF
31	YSA_00336	180045..180980 [+], 936	395443819	hypothetical protein	TssG
32	YSA_00338	180977..181546 [+], 570	395443820	FHA domain-containing protein	Fha
33	YSA_00340	181543..182340 [+], 798	395443821	hypothetical protein	TssJ
34	YSA_00341	182396..183733 [+], 1338	395443822	hypothetical protein	TssK
35	YSA_00343	183735..184604 [+], 870	395443823	hypothetical protein	TssL
36	YSA_00345	184631..184738 [-], 108	395443824	hypothetical protein
37	YSA_00347	184737..185252 [+], 516	395443825	hypothetical protein	TssD
38	YSA_00348	185326..187494 [+], 2169	395443826	Rhs element Vgr protein	TssI
39	YSA_00352	187491..188414 [+], 924	395443827	hypothetical protein
40	YSA_00353	188476..190542 [+], 2067	395443828	hypothetical protein
41	YSA_00356	190613..191395 [+], 783	395443829	hypothetical protein
42	YSA_00358	191398..192291 [+], 894	395443830	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

ECA2866

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	YSA_00347	184737..185252 [+], 516	395443825	hypothetical protein	ECA2866

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Proteins        Genes

N/A

N/A