I. Summary

SecReT6 ID	673
Strain	Enterobacter cloacae subsp. dissolvens SDM
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_018079
Location	2868747..2903913
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

14

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	A3UG_13315	2866102..2866479 [-], 378	392979505	hypothetical protein
2	A3UG_13320	2866618..2867694 [-], 1077	392979506	hypothetical protein
3	A3UG_13325	2867698..2868261 [-], 564	392979507	hypothetical protein
4	A3UG_13330	2868254..2868673 [-], 420	392979508	hypothetical protein
5	A3UG_13335	2868747..2870669 [-], 1923	392979509	hypothetical protein	TssI
6	A3UG_13340	2870735..2871169 [-], 435	392979510	hypothetical protein
7	A3UG_13345	2871259..2873187 [-], 1929	392979511	hypothetical protein	TssI
8	A3UG_13350	2873284..2873631 [-], 348	392979512	hypothetical protein
9	A3UG_13355	2873653..2874708 [-], 1056	392979513	ADP-ribosyl-[dinitrogen reductase] glycohydrolase
10	A3UG_13360	2874760..2875041 [-], 282	392979514	hypothetical protein
11	A3UG_13365	2875128..2875604 [-], 477	392979515	hypothetical protein
12	A3UG_13380	2876416..2877180 [-], 765	392979516	hypothetical protein
13	A3UG_13385	2877177..2878643 [-], 1467	392979517	hypothetical protein
14	A3UG_13390	2878680..2880113 [-], 1434	392979518	hypothetical protein
15	A3UG_13395	2880138..2882753 [-], 2616	392979519	type VI secretion system protein VasG	TssH
16	A3UG_13400	2882792..2883835 [-], 1044	392979520	type VI secretion system protein ImpH	TssG
17	A3UG_13405	2883832..2885703 [-], 1872	392979521	type VI secretion system protein ImpG	TssF
18	A3UG_13410	2885706..2886269 [-], 564	392979522	type VI secretion system protein ImpF	TssE
19	A3UG_13415	2886262..2887074 [-], 813	392979523	type VI secretion system protein ImpE	TagJ
20	A3UG_13420	2887084..2888070 [-], 987	392979524	hypothetical protein
21	A3UG_13425	2888087..2888884 [-], 798	392979525	protein serine/threonine phosphatase
22	A3UG_13430	2888881..2890650 [-], 1770	392979526	FHA domain-containing protein	Fha
23	A3UG_13435	2890738..2891091 [-], 354	392979527	hypothetical protein	ECL_01543 (i)
24	A3UG_13440	2891088..2891579 [-], 492	392979528	putative cytoplasmic protein	ECL_01542 (e)
25	A3UG_13445	2891638..2892120 [-], 483	392979529	type VI secretion system secreted protein Hcp	TssD
26	A3UG_13450	2892301..2892726 [-], 426	392979530	hypothetical protein
27	A3UG_13455	2892723..2893229 [-], 507	392979531	hypothetical protein
28	A3UG_13460	2893295..2894794 [-], 1500	392979532	type VI secretion system protein ImpC	TssC
29	A3UG_13465	2894798..2895322 [-], 525	392979533	hypothetical protein	TssB
30	A3UG_13470	2895398..2896414 [-], 1017	392979534	type VI secretion system protein ImpA	TssA
31	A3UG_13475	2896426..2897142 [-], 717	392979535	type VI secretion system protein ImpM
32	A3UG_13480	2897159..2900779 [-], 3621	392979536	type VI secretion system protein ImpL	TssM
33	A3UG_13485	2900782..2902020 [-], 1239	392979537	hypothetical protein	TssL
34	A3UG_13490	2902038..2903381 [-], 1344	392979538	type VI secretion system protein ImpJ	TssK
35	A3UG_13495	2903410..2903913 [-], 504	392979539	type VI secretion system protein VasD	TssJ
36	A3UG_13500	2903937..2904431 [-], 495	392979540	hypothetical protein
37	A3UG_13505	2905291..2907579 [+], 2289	392979541	hypothetical protein
38	A3UG_13510	2907545..2908336 [-], 792	392979542	putative ABC phosphate/phosphonate transporter, periplasmic ligand binding protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

EC042_4529 , ECL_03140, EC042_4529, ECL_01567, ECL_01542, SMA2263, EC042_4529

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	A3UG_02355	504315..504800 [+], 486	392977332	type VI secretion system effector	EC042_4529
2	A3UG_05760	1263573..1267769 [+], 4197	392978007	Rhs family protein	ECL_03140
3	A3UG_11605	2512938..2513420 [+], 483	392979167	Hcp family type VI secretion system effector	EC042_4529
4	A3UG_13310	2861707..2866098 [-], 4392	392979504	hypothetical protein	ECL_01567
5	A3UG_13440	2891088..2891579 [-], 492	392979528	putative cytoplasmic protein	ECL_01542
6	A3UG_13445	2891638..2892120 [-], 483	392979529	type VI secretion system secreted protein Hcp	SMA2263
7	A3UG_14245	3058632..3059123 [+], 492	392979689	type VI secretion system effector	EC042_4529

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Proteins        Genes

Homology Immunity protein list

ECL_03139, ECL_01543

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	A3UG_05785	1270905..1271489 [+], 585	392978012	hypothetical protein	ECL_03139
2	A3UG_13435	2890738..2891091 [-], 354	392979527	hypothetical protein	ECL_01543

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Proteins        Genes

N/A