I. Summary

SecReT6 ID	675
Strain	Pseudomonas aeruginosa DK2
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_018080
Location	2884928..2904897
Comment
Function	N/A
T6SS Type	Type i; subtype i4a
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

12

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	PADK2_13380	2881639..2882844 [-], 1206	392984072	hypothetical protein
2	PADK2_13385	2883045..2883686 [-], 642	392984073	transcriptional regulator
3	PADK2_13390	2883963..2884358 [+], 396	392984074	hypothetical protein
4	PADK2_13395	2884381..2884917 [-], 537	392984075	hypothetical protein
5	PADK2_13400	2884928..2886832 [-], 1905	392984076	hypothetical protein	TssI
6	PADK2_13405	2886938..2887723 [-], 786	392984077	hypothetical protein
7	PADK2_13410	2887793..2888668 [-], 876	392984078	hypothetical protein
8	PADK2_13415	2888759..2891308 [-], 2550	392984079	ClpA/B-type protease	TssH
9	PADK2_13420	2891310..2892326 [-], 1017	392984080	hypothetical protein	TssG
10	PADK2_13425	2892290..2894083 [-], 1794	392984081	type VI secretion system protein ImpG	TssF
11	PADK2_13430	2894067..2894492 [-], 426	392984082	hypothetical protein	TssE
12	PADK2_13435	2894505..2895002 [-], 498	392984083	hypothetical protein	TssD
13	PADK2_13440	2895076..2896560 [-], 1485	392984084	hypothetical protein	TssC
14	PADK2_13445	2896583..2897128 [-], 546	392984085	hypothetical protein	TssB
15	PADK2_13450	2897337..2897813 [+], 477	392984086	hypothetical protein
16	PADK2_13455	2897912..2899204 [+], 1293	392984087	hypothetical protein	TssK
17	PADK2_13460	2899222..2899980 [+], 759	392984088	type VI secretion system protein ImpK	TssL
18	PADK2_13465	2900192..2903800 [+], 3609	392984089	type VI secretion system protein ImpL	TssM
19	PADK2_13470	2903797..2904897 [+], 1101	392984090	hypothetical protein	TssA
20	PADK2_13475	2905007..2906092 [+], 1086	392984091	transcriptional regulator	Sfa3
21	PADK2_13480	2906328..2906594 [+], 267	392984092	hypothetical protein
22	PADK2_13485	2906754..2907314 [+], 561	392984093	NADH-dependent FMN reductase MsuE
23	PADK2_13490	2907324..2908469 [+], 1146	392984094	methanesulfonate sulfonatase MsuD
24	PADK2_13495	2908499..2909683 [+], 1185	392984095	FMNH2-dependent monooxygenase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PA5089

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PADK2_26940	5827018..5829255 [-], 2238	392986766	hypothetical protein	PA5089

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Proteins        Genes

Homology Immunity protein list

PA5087, PA5088, PA5087

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PADK2_26925	5824383..5825264 [-], 882	392986763	hypothetical protein	PA5087
2	PADK2_26930	5825261..5826142 [-], 882	392986764	hypothetical protein	PA5088
3	PADK2_26935	5826139..5827014 [-], 876	392986765	hypothetical protein	PA5087

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Proteins        Genes

N/A