I. Summary

SecReT6 ID	676
Strain	Pseudomonas aeruginosa DK2
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_018080
Location	3747882..3766857
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	0	1	0	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	PADK2_17325	3743431..3743751 [+], 321	392984859	hypothetical protein
2	PADK2_17330	3743808..3744365 [+], 558	392984860	hypothetical protein
3	PADK2_17335 (folE)	3744443..3744988 [+], 546	392984861	GTP cyclohydrolase I
4	PADK2_17340	3745053..3745514 [-], 462	392984862	hypothetical protein
5	PADK2_17345	3745760..3746140 [+], 381	392984863	hypothetical protein
6	PADK2_17350	3746168..3747157 [-], 990	392984864	serine-threonine kinase Stk1	Stk1
7	PADK2_17355	3747154..3747882 [-], 729	392984865	serine/threonine phosphoprotein phosphatase Stp1	Stp1
8	PADK2_17360	3747882..3751409 [-], 3528	392984866	type VI secretion protein IcmF	TssM
9	PADK2_17365	3751425..3752294 [-], 870	392984867	type VI secretion system protein ImpK	TssL
10	PADK2_17370	3752297..3753628 [-], 1332	392984868	hypothetical protein	TssK
11	PADK2_17375	3753625..3754131 [-], 507	392984869	lipoprotein	TssJ
12	PADK2_17380	3754137..3755330 [-], 1194	392984870	hypothetical protein	Fha
13	PADK2_17385	3755348..3755488 [-], 141	392984871	hypothetical protein
14	PADK2_17390	3755577..3757088 [-], 1512	392984872	transcriptional regulator	Sfa2
15	PADK2_17395	3757099..3759726 [-], 2628	392984873	ClpA/B-type protease	TssH
16	PADK2_17400	3759739..3760746 [-], 1008	392984874	hypothetical protein	TssG
17	PADK2_17405	3760710..3762497 [-], 1788	392984875	hypothetical protein	TssF
18	PADK2_17410	3762835..3763242 [-], 408	392984876	hypothetical protein	TssE
19	PADK2_17415	3763255..3764730 [-], 1476	392984877	hypothetical protein	TssC
20	PADK2_17420	3764759..3765265 [-], 507	392984878	hypothetical protein	TssB
21	PADK2_17425	3765301..3766857 [-], 1557	392984879	hypothetical protein	TssA
22	PADK2_17430	3768029..3768631 [-], 603	392984880	glutathione S-transferase
23	PADK2_17435	3768687..3769853 [-], 1167	392984881	aminotransferase
24	PADK2_17440	3769935..3770411 [+], 477	392984882	transcriptional regulator
25	PADK2_17445	3770462..3771097 [-], 636	392984883	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PSPTO_5435 , PSPTO_5435, PSPTO_5435

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PADK2_01310	290247..290765 [-], 519	392981672	secreted protein Hcp	PSPTO_5435
2	PADK2_18160	3925946..3926464 [+], 519	392985022	secreted protein Hcp	PSPTO_5435
3	PADK2_27865	6032697..6033215 [-], 519	392986949	secreted protein Hcp	PSPTO_5435

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Proteins        Genes

N/A

N/A