I. Summary

SecReT6 ID	677
Strain	Klebsiella oxytoca E718
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_018106
Location	2770900..2795506
Comment
Function	N/A
T6SS Type	Type i; subtype i2
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	2	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

14

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	A225_2620	2765928..2766647 [+], 720	397657634	Aspartate racemase
2	A225_2621	2766676..2767683 [+], 1008	397657635	hypothetical protein
3	A225_2622	2767680..2768450 [+], 771	397657636	hypothetical protein
4	A225_2624	2769504..2770067 [-], 564	397657637	thiJ/PfpI family protein
5	A225_2625	2770134..2770289 [+], 156	397657638	hypothetical protein
6	A225_2626	2770900..2771391 [+], 492	397657639	hypothetical protein	TssB
7	A225_2627	2771426..2772967 [+], 1542	397657640	hypothetical protein	TssC
8	A225_2628	2772981..2774324 [+], 1344	397657641	hypothetical protein	TssK
9	A225_2629	2774321..2774986 [+], 666	397657642	hypothetical protein	TssL
10	A225_2630	2774989..2776671 [+], 1683	397657643	hypothetical protein	TagL
11	A225_2631	2776816..2777307 [+], 492	397657644	hypothetical protein	TssD
12	A225_2632	2777307..2777438 [+], 132	397657645	hypothetical protein
13	A225_2633	2777554..2778141 [+], 588	397657646	clpB protein	TssH
14	A225_2634	2778155..2780197 [+], 2043	397657647	clpB protein	TssH
15	A225_2635	2780194..2782689 [+], 2496	397657648	VgrG protein	TssI
16	A225_2636	2782942..2784909 [+], 1968	397657649	transmembrane protein
17	A225_2637	2785171..2786604 [+], 1434	397657650	hypothetical protein
18	A225_2638	2786745..2789978 [+], 3234	397657651	hypothetical protein	TssM
19	A225_2639	2790074..2790352 [+], 279	397657652	hypothetical protein	PAAR
20	A225_2640	2790365..2792125 [+], 1761	397657653	protein ImpG/VasA	TssF
21	A225_2641	2792089..2793174 [+], 1086	397657654	hypothetical protein	TssG
22	A225_2642	2793194..2793691 [+], 498	397657655	hypothetical protein	TssJ
23	A225_2643	2793693..2794148 [+], 456	397657656	hypothetical protein	TssE
24	A225_2644	2794172..2795506 [+], 1335	397657657	hypothetical protein	TssA
25	A225_2645	2795669..2797348 [-], 1680	397657658	beta-xylosidase
26	A225_2646	2797403..2798857 [-], 1455	397657659	Xyloside transporter XynT
27	A225_2647	2799093..2800301 [+], 1209	397657660	IS10 transposase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

Hcp , EC042_4529

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	A225_0518	535414..535899 [+], 486	397655590	hypothetical protein	Hcp
2	A225_2631	2776816..2777307 [+], 492	397657644	hypothetical protein	EC042_4529

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Proteins        Genes

N/A

N/A