I. Summary

SecReT6 ID	68
Strain	Burkholderia mallei ATCC 23344
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_006349
Location	376517..411529
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BMA_RS17920	371884..372825 [-], 942	490295498	LysR family transcriptional regulator
2	BMA_RS17925	372922..374237 [+], 1316	pseudogene	D-serine ammonia-lyase
3	BMA_RS17930	374408..375322 [-], 915	490294974	LysR family transcriptional regulator
4	BMA_RS17935	375504..376208 [+], 705	490295326	SDR family oxidoreductase
5	BMA_RS26115	376770..376946 [-], 177	490295390	hypothetical protein
6	BMA_RS17940	376995..377312 [-], 318	490310751	chorismate lyase
7	BMA_RS17945	377249..377494 [-], 246	1121458858	chorismate lyase
8	BMA_RS17950	377622..378857 [-], 1236	741014115	flavin-dependent oxidoreductase
9	BMA_RS17955	379131..379316 [-], 186	490295378	hypothetical protein
10	BMA_RS17960	379335..381233 [+], 1899	490308269	DUF3857 and transglutaminase domain-containing protein
11	BMA_RS17965 (tagH)	381276..382712 [-], 1437	490294955	type VI secretion system-associated FHA domain protein TagH
12	BMA_RS17970	382767..383168 [-], 402	490294824	TssQ family T6SS-associated lipoprotein
13	BMA_RS17975 (tssJ)	383808..384326 [+], 519	490295246	type VI secretion system lipoprotein TssJ
14	BMA_RS17980 (tssK)	384323..385726 [+], 1404	490295036	type VI secretion system baseplate subunit TssK
15	BMA_RS17985	385742..387058 [+], 1317	490295223	DotU family type VI secretion system protein
16	BMA_RS17990 (tssM)	387061..390690 [+], 3630	490295215	type VI secretion system membrane subunit TssM
17	BMA_RS17995 (tagF)	390687..391602 [+], 916	pseudogene	type VI secretion system-associated protein TagF
18	BMA_RS18000	391587..391814 [-], 228	490294720	hypothetical protein
19	BMA_RS18005	391813..394410 [+], 2598	490295332	protein kinase
20	BMA_RS18010 (tssA)	394463..395542 [+], 1080	490295524	type VI secretion system protein TssA
21	BMA_RS18015 (tssB)	395605..396183 [+], 579	490295193	type VI secretion system contractile sheath small subunit
22	BMA_RS18020 (tssC)	396176..397684 [+], 1509	490295385	type VI secretion system contractile sheath large subunit
23	BMA_RS18025	397744..398235 [+], 492	490295232	type VI secretion system tube protein Hcp
24	BMA_RS18030 (tssE)	398254..398814 [+], 561	490300750	type VI secretion system baseplate subunit TssE
25	BMA_RS18035 (tssF)	398819..400690 [+], 1872	490294960	type VI secretion system baseplate subunit TssF
26	BMA_RS18040 (tssG)	400687..402177 [+], 1491	490300751	type VI secretion system baseplate subunit TssG
27	BMA_RS18045 (tssH)	402156..404810 [+], 2655	490295248	type VI secretion system ATPase TssH
28	BMA_RS18050 (tssI)	404807..407011 [+], 2205	490308264	type VI secretion system tip protein VgrG
29	BMA_RS18055	407086..407739 [+], 654	490300753	hypothetical protein
30	BMA_RS18060	407669..408727 [+], 1059	490300756	RHS repeat protein
31	BMA_RS18065	408755..409875 [-], 1121	741020948	IS3 family transposase
32	BMA_RS18070	409943..410536 [-], 594	pseudogene	acyltransferase family protein
33	BMA_RS18075	410549..411529 [-], 981	499517700	methyltransferase domain-containing protein
34	BMA_RS18080	412046..413470 [+], 1425	490295311	cytosine permease
35	BMA_RS18085	413467..414405 [+], 939	490294691	NAD-dependent protein deacetylase
36	BMA_RS18090	414368..415045 [+], 678	490294911	DUF938 domain-containing protein
37	BMA_RS18095	415369..415512 [-], 144	490295039	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

N/A

N/A

(1) Schell MA et al. (2007). Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 64(6):1466-85. [PudMed:17555434]

This literature contains experimental investigation