I. Summary

SecReT6 ID	688
Strain	Burkholderia cepacia GG4
Replicon	chromosome I [Browse all T6SS(s) in this replicon]
Accession	NC_018513
Location	3314859..3334721
Comment
Function	N/A
T6SS Type	Type i; subtype i4b
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	0	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	GEM_3031	3311401..3312429 [-], 1029	402567776	Mg2 transporter protein CorA family protein
2	GEM_3032	3312638..3313591 [-], 954	402567777	tail fiber protein
3	GEM_3033	3313713..3314558 [+], 846	402567778	peptidase M15B and M15C DD-carboxypeptidase VanY/endolysin
4	GEM_3034	3314859..3318800 [+], 3942	402567779	type VI secretion protein IcmF	TssM
5	GEM_3035	3318797..3319786 [+], 990	402567780	hypothetical protein
6	GEM_3036	3319791..3320747 [+], 957	402567781	OmpA/MotB domain-containing protein	TagL
7	GEM_3037	3320825..3321946 [-], 1122	402567782	type VI secretion-associated protein, ImpA family	TssA
8	GEM_3038	3321986..3324655 [-], 2670	402567783	type VI secretion ATPase, ClpV1 family	TssH
9	GEM_3039	3324700..3325800 [-], 1101	402567784	type VI secretion system protein	TssG
10	GEM_3040	3325764..3327599 [-], 1836	402567785	type VI secretion system protein	TssF
11	GEM_3041	3327677..3328162 [-], 486	402567786	type VI secretion system protein TssE	TssE
12	GEM_3042	3328225..3328728 [-], 504	402567787	type VI secretion system protein	TssD
13	GEM_3043	3328799..3330289 [-], 1491	402567788	type VI secretion protein	TssC
14	GEM_3044	3330305..3330820 [-], 516	402567789	type VI secretion system protein	TssB
15	GEM_3045	3330865..3331503 [-], 639	402567790	Tetratricopeptide TPR 2 repeat protein
16	GEM_3046	3331894..3332493 [+], 600	402567791	type VI secretion system protein	TssJ
17	GEM_3047	3332596..3333942 [+], 1347	402567792	type VI secretion system protein	TssK
18	GEM_3048	3333939..3334721 [+], 783	402567793	transmembrane protein	TssL
19	GEM_3049	3334808..3335125 [-], 318	402567794	hypothetical protein
20	GEM_3050	3335465..3336265 [+], 801	402567795	extracellular solute-binding protein
21	GEM_3051	3336347..3336550 [-], 204	402567796	hypothetical protein
22	GEM_3052	3336869..3337894 [+], 1026	402567797	XRE family transcriptional regulator

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

BTH_I2693 , BTH_I2697, BTH_I2698, BTH_I2705, BCAL0343

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	GEM_2076	2264523..2267327 [-], 2805	402566842	rhs element vgr	BTH_I2693
2	GEM_2080	2270338..2274066 [-], 3729	402566846	rhs element vgr	BTH_I2697
3	GEM_2081	2274128..2276527 [-], 2400	402566847	secreted protein hcp	BTH_I2698
4	GEM_2084	2278097..2280892 [-], 2796	402566850	VgrG protein	BTH_I2705
5	GEM_3042	3328225..3328728 [-], 504	402567787	type VI secretion system protein	BCAL0343

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Proteins        Genes

N/A

N/A