I. Summary

SecReT6 ID	69
Strain	Burkholderia mallei ATCC 23344
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_006349
Location	429068..465354
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	0	1	1	2	0	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	0	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BMAA0429	425119..426153 [+], 1035	53716568	lipase chaperone
2	BMAA0430	426255..427775 [-], 1521	53716567	GntR family transcriptional regulator
3	BMAA0431	427893..428993 [+], 1101	53716566	spermidine/putrescine ABC transporter, ATP-binding protein
4	BMAA0432	429068..430114 [+], 1047	53716565	spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein
5	BMAA0433	430215..431474 [+], 1260	53716564	spermidine/putrescine ABC transporter, permease protein
6	BMAA0435	432426..432884 [-], 459	77358925	serine protease
7	BMAA0436	432906..433739 [-], 834	53716557	IS407A, transposase OrfB
8	BMAA0437	433763..434026 [-], 264	53717423	IS407A, transposase OrfA
9	BMAA0438	434138..434563 [+], 426	77358923	hypothetical protein	TssB
10	BMAA0439	434560..436071 [+], 1512	53716556	hypothetical protein	TssC
11	BMAA0440	436215..436742 [+], 528	77358921	hypothetical protein	TssD
12	BMAA0441	436822..437253 [+], 432	77358919	hypothetical protein	TssE
13	BMAA0442	437267..439129 [+], 1863	77358918	hypothetical protein	TssF
14	BMAA0443	439126..440115 [+], 990	77358917	hypothetical protein	TssG
15	BMAA0445	442979..445225 [+], 2247	53716549	Rhs element Vgr protein	TssI
16	BMAA0446	445391..447679 [+], 2289	77358915	Rhs element Vgr protein	TssI
17	BMAA0447	448268..449899 [+], 1632	53716548	hypothetical protein
18	BMAA0448	449899..450969 [+], 1071	53716547	pentapeptide repeat-containing protein
19	BMAA0449	450972..451688 [+], 717	53716546	hypothetical protein
20	BMAA0450	451731..452120 [+], 390	77358914	hypothetical protein
21	BMAA0451	452126..452719 [+], 594	53716545	lipoprotein	TssJ
22	BMAA0453	454103..455818 [+], 1716	53716506	hypothetical protein	TssL
23	BMAA0454	455875..459318 [+], 3444	53716505	hypothetical protein	TssM
24	BMAA0455	459377..459736 [+], 360	77358798	hypothetical protein
25	BMAA0456	459824..460327 [+], 504	53716504	hypothetical protein
26	BMAA0457	460387..460548 [+], 162	77358794	hypothetical protein
27	BMAA0458	460878..461051 [-], 174	77358793	hypothetical protein
28	BMAA0459 (rhlA-1)	461331..462230 [+], 900	53716496	rhamnosyltransferase I, subunit A
29	BMAA0460 (rhlB-1)	462452..463771 [+], 1320	53717492	rhamnosyltransferase I, subunit B
30	BMAA0461	463768..465354 [+], 1587	53717491	multidrug resistance protein
31	BMAA0462 (rhlC)	465619..466614 [+], 996	53717489	rhamnosyltransferase II
32	BMAA0463	466919..468514 [+], 1596	53717488	RND efflux system outer membrane lipoprotein
33	BMAA0464	469082..470248 [+], 1167	53717493	multidrug resistance protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files

Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

YPK_3563

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BMAA0440	436215..436742 [+], 528	77358921	hypothetical protein	YPK_3563

Download FASTA format files

Proteins        Genes

N/A

(1) Schell MA et al. (2007). Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 64(6):1466-85. [PudMed:17555434]

This literature contains experimental investigation