I. Summary

SecReT6 ID	69
Strain	Burkholderia mallei ATCC 23344
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_006349
Location	429068..465354
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	0	1	1	2	0	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	0	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BMA_RS18145	425119..426153 [+], 1035	490294883	lipase secretion chaperone
2	BMA_RS18150	426255..427784 [-], 1530	490305476	PLP-dependent aminotransferase family protein
3	BMA_RS18155	427893..428993 [+], 1101	490295292	ABC transporter ATP-binding protein
4	BMA_RS18160	429068..430114 [+], 1047	490295316	ABC transporter substrate-binding protein
5	BMA_RS18165	430215..431474 [+], 1260	490300774	ABC transporter permease
6	BMA_RS18170	431505..432331 [+], 827	pseudogene	ABC transporter permease
7	BMA_RS18175	432426..432857 [-], 432	pseudogene	peptidase S53
8	BMA_RS18180	432906..434026 [-], 1121	741020948	IS3 family transposase
9	BMA_RS18185 (tssB)	434096..434563 [+], 468	pseudogene	type VI secretion system contractile sheath small subunit
10	BMA_RS18190 (tssC)	434566..436071 [+], 1506	490295382	type VI secretion system contractile sheath large subunit
11	BMA_RS18195	436215..436742 [+], 528	490300780	type VI secretion system tube protein Hcp
12	BMA_RS18200	436822..437253 [+], 432	490295415	GPW/gp25 family protein
13	BMA_RS18205 (tssF)	437267..439129 [+], 1863	490300782	type VI secretion system baseplate subunit TssF
14	BMA_RS18210 (tssG)	439126..440115 [+], 990	490295387	type VI secretion system baseplate subunit TssG
15	BMA_RS18215 (tssH)	440118..442988 [+], 2871	pseudogene	type VI secretion system ATPase TssH
16	BMA_RS18220 (vgrG)	442979..445225 [+], 2247	490300787	type VI secretion system tip protein VgrG
17	BMA_RS18225 (vgrG)	445391..447679 [+], 2289	490295014	type VI secretion system tip protein VgrG
18	BMA_RS18230	447683..449899 [+], 2217	490305479	pentapeptide repeat-containing protein
19	BMA_RS18235	449899..450969 [+], 1071	490307160	pentapeptide repeat-containing protein
20	BMA_RS18240	450972..451688 [+], 717	490387208	DUF3540 domain-containing protein
21	BMA_RS18245	451731..452120 [+], 390	490295064	DUF4150 domain-containing protein
22	BMA_RS18250 (tssK)	452305..453450 [+], 1146	490300801	type VI secretion system baseplate subunit TssK
23	BMA_RS18255	453469..454077 [+], 609	490311553	type VI secretion system baseplate subunit TssK
24	BMA_RS18260	454160..455818 [+], 1659	490294913	type IVB secretion system protein IcmH/DotU
25	BMA_RS18265 (tssM)	455815..459318 [+], 3504	490294712	type VI secretion system membrane subunit TssM
26	BMA_RS18270	459377..459736 [+], 360	490295399	hypothetical protein
27	BMA_RS18275	459759..460184 [+], 426	490294922	hypothetical protein
28	BMA_RS18280	460505..460780 [+], 276	490295280	hypothetical protein
29	BMA_RS18285	461331..462230 [+], 900	490291378	alpha/beta hydrolase
30	BMA_RS18290	462272..462562 [-], 291	490305952	hypothetical protein
31	BMA_RS18295	462452..463771 [+], 1320	490291357	glycosyltransferase
32	BMA_RS18300	463768..465354 [+], 1587	490288762	DHA2 family efflux MFS transporter permease subunit
33	BMA_RS18305	465619..466614 [+], 996	490291223	glycosyltransferase family 2 protein
34	BMA_RS18315	466919..468514 [+], 1596	490300954	efflux transporter outer membrane subunit
35	BMA_RS18320	468995..470248 [+], 1254	490291248	efflux RND transporter periplasmic adaptor subunit

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

YPK_3563

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

N/A

(1) Schell MA et al. (2007). Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 64(6):1466-85. [PudMed:17555434]

This literature contains experimental investigation