I. Summary

SecReT6 ID	693
Strain	Burkholderia pseudomallei BPC006
Replicon	chromosome I [Browse all T6SS(s) in this replicon]
Accession	NC_018527
Location	3575917..3595902
Comment
Function	N/A
T6SS Type	Type i; subtype i4b
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	0	0	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

11

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BPC006_I3700	3572042..3573490 [+], 1449	403520316	TolC family type I secretion outer membrane protein
2	BPC006_I3701	3573487..3573624 [+], 138	403520317	hypothetical protein
3	BPC006_I3702	3573700..3574668 [-], 969	403520318	hypothetical protein
4	BPC006_I3703	3574680..3575645 [+], 966	403520319	D-alanyl-D-alanine carboxypeptidase
5	BPC006_I3704	3575796..3575909 [+], 114	403520320	hypothetical protein
6	BPC006_I3705	3575917..3579810 [+], 3894	403520321	hypothetical protein	TssM
7	BPC006_I3706	3579807..3580796 [+], 990	403520322	hypothetical protein
8	BPC006_I3707	3580801..3581733 [+], 933	403520323	ompA family protein	TagL
9	BPC006_I3709	3583143..3585812 [-], 2670	403520324	ClpA/B type protease	TssH
10	BPC006_I3710	3585846..3586946 [-], 1101	403520325	hypothetical protein	TssG
11	BPC006_I3711	3586910..3588748 [-], 1839	403520326	hypothetical protein	TssF
12	BPC006_I3712	3588828..3589310 [-], 483	403520327	hypothetical protein	TssE
13	BPC006_I3713	3589368..3589862 [-], 495	403520328	hypothetical protein	TssD
14	BPC006_I3714	3589944..3591434 [-], 1491	403520329	hypothetical protein	TssC
15	BPC006_I3715	3591451..3591969 [-], 519	403520330	hypothetical protein	TssB
16	BPC006_I3716	3592006..3592641 [-], 636	403520331	hypothetical protein
17	BPC006_I3717	3592803..3592940 [-], 138	403520332	hypothetical protein
18	BPC006_I3718	3593051..3593665 [+], 615	403520333	lipoprotein	TssJ
19	BPC006_I3719	3593774..3595120 [+], 1347	403520334	hypothetical protein	TssK
20	BPC006_I3720	3595117..3595902 [+], 786	403520335	hypothetical protein	TssL
21	BPC006_I3721	3596004..3596369 [+], 366	403520336	phage tail completion protein
22	BPC006_I3722	3597458..3597874 [-], 417	403520337	DNA methylase
23	BPC006_I3723	3597959..3598156 [-], 198	403520338	bacteriophage tail protein I
24	BPC006_I3724	3598363..3598662 [-], 300	403520339	bacteriophage baseplate assembly protein J
25	BPC006_I3725	3598976..3599584 [-], 609	403520340	ISBma1, transposase
26	BPC006_I3726	3599548..3600195 [-], 648	403520341	ISBma1, transposase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

BTH_I0068 , BTH_I2705, BTH_I2698, BTH_I2697, BTH_I2693, BCAL0343, BTH_I3225, BTH_II0090, BTH_I3226, BTH_I3225, BTH_II0310

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BPC006_I0077	75552..76520 [+], 969	403516745	hypothetical protein	BTH_I0068
2	BPC006_I1650	1586894..1589701 [+], 2808	403518304	Rhs element Vgr protein	BTH_I2705
3	BPC006_I1654	1592179..1594227 [+], 2049	403518308	hypothetical protein	BTH_I2698
4	BPC006_I1656	1594336..1597671 [+], 3336	403518310	Rhs element Vgr protein	BTH_I2697
5	BPC006_I1660	1599807..1602647 [+], 2841	403518314	Rhs element Vgr protein	BTH_I2693
6	BPC006_I3713	3589368..3589862 [-], 495	403520328	hypothetical protein	BCAL0343
7	BPC006_II0102	88359..91139 [+], 2781	403521396	Rhs element Vgr protein	BTH_I3225
8	BPC006_II0103	91153..93393 [+], 2241	403521397	Type V secretory pathway, adhesin AidA	BTH_II0090
9	BPC006_II1612	1635452..1637692 [-], 2241	403522893	hypothetical protein	BTH_I3226
10	BPC006_II1613	1637706..1640492 [-], 2787	403522894	Rhs element Vgr protein	BTH_I3225
11	BPC006_II2723	2746518..2747423 [+], 906	403524001	hypothetical protein	BTH_II0310

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Proteins        Genes

Homology Immunity protein list

BTH_I0069, BTH_I2700

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BPC006_I0078	76507..76875 [+], 369	403516746	lipoprotein	BTH_I0069
2	BPC006_I1652	1590723..1591232 [+], 510	403518306	hypothetical protein	BTH_I2700

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Proteins        Genes

N/A