I. Summary
SecReT6 ID70 experimental
StrainBurkholderia mallei ATCC 23344
Repliconchromosome II [Browse all T6SS(s) in this replicon]
CommentVirulence in Syrian hamster model; intracellular growth, actin polymerisation and mononuclear giant cell formation in cultured macrophages
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1BMAA0722732317..732895 [-], 57953717353hypothetical protein 
2BMAA0723732932..733852 [-], 92177358896hypothetical protein 
3BMAA0724733927..734760 [-], 83453717354IS407A, transposase OrfB 
4BMAA0725734784..735047 [-], 26453717476IS407A, transposase OrfA 
5BMAA0727 (folE)735641..736273 [-], 63353717514GTP cyclohydrolase I 
6BMAA0728736278..736571 [-], 29453717361hypothetical protein 
7BMAA0729736735..738714 [-], 198077358950hypothetical protein  BMAA0729 (e)
8BMAA0729.1738692..743269 [-], 457877358930hypothetical protein  TssM
9BMAA0731742735..743400 [-], 66677358952hypothetical protein  TssL
10BMAA0732743397..744794 [-], 139877358799hypothetical protein  TssK
11BMAA0733744827..745624 [-], 79853717587hypothetical protein  TssJ
12BMAA0734745634..746026 [-], 39353717362hypothetical protein 
13BMAA0735746055..746816 [-], 76253717588hypothetical protein 
14BMAA0737750563..753586 [-], 302453717363Rhs element Vgr protein  TssI
15BMAA0738753613..756696 [-], 308453717364ATP-dependent Clp protease, ATP-binding subunit ClpB  TssH
16BMAA0739756683..757705 [-], 102353717365hypothetical protein  TssG
17BMAA0740757693..759435 [-], 174377358961hypothetical protein  TssF
18BMAA0741759472..759933 [-], 46277358962hypothetical protein  TssE
19BMAA0742759926..760435 [-], 51077358963hypothetical protein  TssD
20BMAA0743760655..762154 [-], 150077358966hypothetical protein  TssC
21BMAA0744762178..762672 [-], 49577358965hypothetical protein  TssB
22BMAA0745763148..764776 [+], 162953717366sensor histidine kinase  VirA
23BMAA0746 (virG)764773..765510 [+], 73853717367DNA-binding response regulator  VirG
24BMAA0747765507..767435 [+], 192953717376hypothetical protein  TssA
25BMAA0748767778..768155 [-], 37877358970hypothetical protein 
26BMAA0749768235..769356 [-], 112253717377hemagglutinin domain-containing protein 
27BMAA0750769385..770605 [-], 122153717378hypothetical protein 
28BMAA0751770893..771918 [-], 102653717379N-acetylmuramoyl-L-alanine amidase 
29BMAA0752772063..772695 [-], 63353717380hypothetical protein 
30BMAA0753772957..773571 [-], 61553717388SCO1/SenC family protein 
31BMAA0754773568..774359 [-], 79253717381hypothetical protein 
32BMAA0755774429..775991 [-], 156353717382outer membrane nitrite reductase 
33BMAA0756776399..776839 [-], 44153717389hypothetical protein 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
BMAA0737 , BMAA0729, BMAA0742

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1BMAA0737750563..753586 [-], 302453717363Rhs element Vgr protein BMAA0737
2BMAA0729736735..738714 [-], 198077358950hypothetical protein BMAA0729
3BMAA0742759926..760435 [-], 51077358963hypothetical protein BMAA0742
Data from experimental literature

Download FASTA format files
Proteins        Genes
Regulatory element identified
VirA (BMAA0745)ActivatorPMID: 17555434
VirG (BMAA0746)ActivatorPMID: 17555434

BMAA1517ActivatorPMID: 17555434

The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1BMAA0746 (VirG)BMAA0729.1 (TssM1) expression.
2BMAA0746 (VirG)TCS+BMAA0742 (Hcp1) expressionAcid pH upregulates TssM and Hcp1 expression through VirAG.
3BMAA0745 (VirA)TCS+BMAA0746 (VirG)Acid pH upregulates TssM and Hcp1 expression through VirAG.
(1) Fisher NA et al. (2012). The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 12:117. [PudMed:22892068]
(2) Burtnick MN et al. (2010). Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 78(1):88-99. [PudMed:19884331] experimental
(3) Shanks J et al. (2009). Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 77(4):1636-48. [PudMed:19168747] experimental
(4) Schell MA et al. (2007). Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 64(6):1466-85. [PudMed:17555434] experimental
experimental This literature contains experimental investigation