I. Summary

SecReT6 ID	70
Strain	Burkholderia mallei ATCC 23344
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_006349
Location	735641..772695
Comment	Virulence in Syrian hamster model; intracellular growth, actin polymerisation and mononuclear giant cell formation in cultured macrophages
Function	anti-eukaryotic; effector translocation;virulence
T6SS Type	Type i; subtype i1
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BMAA0722	732317..732895 [-], 579	53717353	hypothetical protein
2	BMAA0723	732932..733852 [-], 921	77358896	hypothetical protein
3	BMAA0724	733927..734760 [-], 834	53717354	IS407A, transposase OrfB
4	BMAA0725	734784..735047 [-], 264	53717476	IS407A, transposase OrfA
5	BMAA0727 (folE)	735641..736273 [-], 633	53717514	GTP cyclohydrolase I
6	BMAA0728	736278..736571 [-], 294	53717361	hypothetical protein
7	BMAA0729	736735..738714 [-], 1980	77358950	hypothetical protein	BMAA0729 (e)
8	BMAA0729.1	738692..743269 [-], 4578	77358930	hypothetical protein	TssM
9	BMAA0731	742735..743400 [-], 666	77358952	hypothetical protein	TssL
10	BMAA0732	743397..744794 [-], 1398	77358799	hypothetical protein	TssK
11	BMAA0733	744827..745624 [-], 798	53717587	hypothetical protein	TssJ
12	BMAA0734	745634..746026 [-], 393	53717362	hypothetical protein
13	BMAA0735	746055..746816 [-], 762	53717588	hypothetical protein
14	BMAA0737	750563..753586 [-], 3024	53717363	Rhs element Vgr protein	TssI
15	BMAA0738	753613..756696 [-], 3084	53717364	ATP-dependent Clp protease, ATP-binding subunit ClpB	TssH
16	BMAA0739	756683..757705 [-], 1023	53717365	hypothetical protein	TssG
17	BMAA0740	757693..759435 [-], 1743	77358961	hypothetical protein	TssF
18	BMAA0741	759472..759933 [-], 462	77358962	hypothetical protein	TssE
19	BMAA0742	759926..760435 [-], 510	77358963	hypothetical protein	TssD
20	BMAA0743	760655..762154 [-], 1500	77358966	hypothetical protein	TssC
21	BMAA0744	762178..762672 [-], 495	77358965	hypothetical protein	TssB
22	BMAA0745	763148..764776 [+], 1629	53717366	sensor histidine kinase	VirA
23	BMAA0746 (virG)	764773..765510 [+], 738	53717367	DNA-binding response regulator	VirG
24	BMAA0747	765507..767435 [+], 1929	53717376	hypothetical protein	TssA
25	BMAA0748	767778..768155 [-], 378	77358970	hypothetical protein
26	BMAA0749	768235..769356 [-], 1122	53717377	hemagglutinin domain-containing protein
27	BMAA0750	769385..770605 [-], 1221	53717378	hypothetical protein
28	BMAA0751	770893..771918 [-], 1026	53717379	N-acetylmuramoyl-L-alanine amidase
29	BMAA0752	772063..772695 [-], 633	53717380	hypothetical protein
30	BMAA0753	772957..773571 [-], 615	53717388	SCO1/SenC family protein
31	BMAA0754	773568..774359 [-], 792	53717381	hypothetical protein
32	BMAA0755	774429..775991 [-], 1563	53717382	outer membrane nitrite reductase
33	BMAA0756	776399..776839 [-], 441	53717389	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

BMAA0737 , BMAA0729, BMAA0742

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BMAA0737	750563..753586 [-], 3024	53717363	Rhs element Vgr protein	BMAA0737
2	BMAA0729	736735..738714 [-], 1980	77358950	hypothetical protein	BMAA0729
3	BMAA0742	759926..760435 [-], 510	77358963	hypothetical protein	BMAA0742
Data from experimental literature

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Proteins        Genes

N/A

Regulatory element identified

VirG

Regulator	Description	Reference
VirA (BMAA0745)	Activator	PMID: 17555434
VirG (BMAA0746)	Activator	PMID: 17555434

BMAA1517

Regulator	Description	Reference
BMAA1517	Activator	PMID: 17555434

The information on regulator interaction.

#	homolog effector locus tag	Regulator way	Function	Regulate target	Description
1	BMAA0746 (VirG)			BMAA0729.1 (TssM1) expression	.
2	BMAA0746 (VirG)	TCS	+	BMAA0742 (Hcp1) expression	Acid pH upregulates TssM and Hcp1 expression through VirAG.
3	BMAA0745 (VirA)	TCS	+	BMAA0746 (VirG)	Acid pH upregulates TssM and Hcp1 expression through VirAG.

(1) Fisher NA et al. (2012). The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 12:117. [PudMed:22892068]

(2) Burtnick MN et al. (2010). Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 78(1):88-99. [PudMed:19884331]

(3) Shanks J et al. (2009). Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 77(4):1636-48. [PudMed:19168747]

(4) Schell MA et al. (2007). Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 64(6):1466-85. [PudMed:17555434]

This literature contains experimental investigation