I. Summary
SecReT6 ID70 experimental
StrainBurkholderia mallei ATCC 23344
Repliconchromosome II [Browse all T6SS(s) in this replicon]
CommentVirulence in Syrian hamster model; intracellular growth, actin polymerisation and mononuclear giant cell formation in cultured macrophages
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1BMA_RS19500732317..732814 [-], 498490306826hypothetical protein 
2BMA_RS19505732932..733816 [-], 885490387224metal-dependent hydrolase 
3BMA_RS19510733927..735047 [-], 1121741020948IS3 family transposase 
4BMA_RS19515735241..735548 [-], 308pseudogeneDDE-type integrase/transposase/recombinase 
5BMA_RS19520735641..736273 [-], 633490663813GTP cyclohydrolase I 
6BMA_RS19525736278..736571 [-], 294490687372type VI secretion system protein TssN 
7BMA_RS19530736530..736775 [+], 246490306833hypothetical protein 
8BMA_RS19535736735..737958 [-], 12241807434233Ig-like domain-containing protein 
9BMA_RS19540 (tssM-5)738692..742738 [-], 40471859203766type VI secretion system membrane subunit TssM-5 
10BMA_RS19545742735..743400 [-], 666490289278type VI secretion system protein TssL 
11BMA_RS19550 (tssK)743397..744794 [-], 1398490311820type VI secretion system baseplate subunit TssK 
12BMA_RS19555 (tssJ)744827..745624 [-], 798490305833type VI secretion system lipoprotein TssJ 
13BMA_RS19560745634..746026 [-], 393490305834type VI secretion system protein TssI 
14BMA_RS19565746055..746816 [-], 762490305837type VI secretion system protein TssH 
15BMA_RS19570746813..747877 [-], 1065490311822type VI secretion system protein TssG 
16BMA_RS19575747895..750537 [-], 2643490311823type VI secretion system protein TssF 
17BMA_RS19580 (tssI)750563..753586 [-], 3024490289303type VI secretion system tip protein VgrG 
18BMA_RS19585753613..756696 [-], 3084490305838AAA family ATPase 
19BMA_RS19590 (tssG)756683..757705 [-], 1023490305839type VI secretion system baseplate subunit TssG 
20BMA_RS19595 (tssF)757693..759435 [-], 1743490305840type VI secretion system baseplate subunit TssF 
21BMA_RS19600 (tssE)759472..759933 [-], 462490289134type VI secretion system baseplate subunit TssE 
22BMA_RS19605 (hcp1)759926..760435 [-], 510490305841type VI secretion system effector Hcp1 
23BMA_RS19610 (tssC)760655..762181 [-], 1527490305843type VI secretion system contractile sheath large subunit 
24BMA_RS19615 (tssB)762178..762672 [-], 495490305844type VI secretion system contractile sheath small subunit 
25BMA_RS19620762932..764776 [+], 1845490305845MASE1 domain-containing protein 
26BMA_RS19625764773..765510 [+], 738490311617response regulator transcription factor 
27BMA_RS19630 (tssA)765501..767435 [+], 1935490305847type VI secretion system protein TssA 
28BMA_RS19635767778..768155 [-], 378490289131hypothetical protein 
29BMA_RS19640768235..769356 [-], 1122490386950YadA-like family protein 
30BMA_RS19645769385..770746 [-], 1362490305851autotransporter strand-loop-strand O-heptosyltransferase 
31BMA_RS19650770893..771918 [-], 1026490311629N-acetylmuramoyl-L-alanine amidase 
32BMA_RS19655772063..772452 [-], 390490289046hypothetical protein 
33BMA_RS19660772957..773571 [-], 615490305853SCO family protein 
34BMA_RS19665773568..774404 [-], 837490305854formylglycine-generating enzyme family protein 
35BMA_RS19670 (nirK)774429..775991 [-], 1563490305855nitrite reductase, copper-containing 
36BMA_RS19675776399..776839 [-], 441490289301SRPBCC family protein 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
BMAA0737 , BMAA0729, BMAA0742

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes
Regulatory element identified
VirA (BMAA0745)ActivatorPMID: 17555434
VirG (BMAA0746)ActivatorPMID: 17555434

BMAA1517ActivatorPMID: 17555434

The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1BMAA0746 (VirG)BMAA0729.1 (TssM1) expression.
2BMAA0746 (VirG)TCS+BMAA0742 (Hcp1) expressionAcid pH upregulates TssM and Hcp1 expression through VirAG.
3BMAA0745 (VirA)TCS+BMAA0746 (VirG)Acid pH upregulates TssM and Hcp1 expression through VirAG.
(1) Fisher NA et al. (2012). The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 12:117. [PudMed:22892068]
(2) Burtnick MN et al. (2010). Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 78(1):88-99. [PudMed:19884331] experimental
(3) Shanks J et al. (2009). Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 77(4):1636-48. [PudMed:19168747] experimental
(4) Schell MA et al. (2007). Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 64(6):1466-85. [PudMed:17555434] experimental
experimental This literature contains experimental investigation