I. Summary

SecReT6 ID	704
Strain	Escherichia coli O104:H4 str. 2009EL-2071
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_018661
Location	4205139..4246669
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	2	1	1	2	1	2	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	O3O_05095 (prfH)	4201568..4202182 [-], 615	407467709	peptide chain release factor-like protein
2	O3O_05090	4202179..4203318 [-], 1140	407467708	hypothetical protein
3	O3O_05085	4203564..4204016 [-], 453	407467707	acyltransferase
4	O3O_05080	4204013..4205068 [-], 1056	407467706	DNA polymerase IV
5	O3O_05075	4205139..4205924 [-], 786	407467705	hypothetical protein	TagL
6	O3O_05070	4205869..4207608 [+], 1740	407467704	flagellar system protein
7	O3O_05065	4207713..4207991 [+], 279	407467703	hypothetical protein
8	O3O_05060	4207984..4208340 [+], 357	407467702	HicB
9	O3O_05055	4208397..4209146 [-], 750	407467701	hydrolase
10	O3O_05050	4209356..4209616 [+], 261	407467700	DNA-damage-inducible protein J
11	O3O_05045	4209619..4209897 [+], 279	407467699	toxin of the YafQ-DinJ toxin-antitoxin system
12	O3O_05040	4210053..4210793 [+], 741	407467698	hypothetical protein
13	O3O_05035	4210764..4211531 [-], 768	407467697	amidotransfease
14	O3O_05030 (gmhA)	4211737..4212315 [-], 579	407467696	phosphoheptose isomerase
15	O3O_05025 (fadE)	4212555..4214999 [+], 2445	407467695	acyl-CoA dehydrogenase
16	O3O_05020	4215042..4215515 [-], 474	407467694	C-lysozyme inhibitor
17	O3O_05015	4215669..4216439 [+], 771	407467693	C-N hydrolase family amidase
18	O3O_05010	4216481..4217524 [-], 1044	407467692	transposase
19	O3O_04995	4219027..4219509 [-], 483	407467691	hypothetical protein
20	O3O_04990	4219493..4220683 [-], 1191	407467690	Rhs Core protein
21	O3O_04985	4220652..4223156 [-], 2505	407467689	Rhs core protein	PAAR
22	O3O_04980	4223232..4225373 [-], 2142	407467688	hypothetical protein	TssI
23	O3O_04975	4225583..4226101 [-], 519	407467687	hypothetical protein	TssD
24	O3O_04970	4226228..4226371 [-], 144	407467686	hypothetical protein
25	O3O_04965	4226798..4227298 [+], 501	407467685	hypothetical protein	TssB
26	O3O_04960	4227333..4227557 [+], 225	407467684	hypothetical protein	TssC
27	O3O_04955	4227608..4229083 [+], 1476	407467683	hypothetical protein	TssC
28	O3O_04950	4229090..4229503 [+], 414	407467682	hypothetical protein	TssE
29	O3O_04945	4229507..4231357 [+], 1851	407467681	type VI secretion system protein ImpG	TssF
30	O3O_04940	4231321..4232403 [+], 1083	407467680	hypothetical protein	TssG
31	O3O_04935	4232428..4233708 [+], 1281	407467679	type VI secretion system protein ImpI	Fha
32	O3O_04930	4233705..4234229 [+], 525	407467678	lipoprotein	TssJ
33	O3O_04925	4234232..4235563 [+], 1332	407467677	hypothetical protein	TssK
34	O3O_04920	4235568..4236329 [+], 762	407467676	membrane protein Aec26	TssL
35	O3O_04915	4236338..4239097 [+], 2760	407467675	ATP-dependent Clp proteinase ATP-binding subunit	TssH
36	O3O_04910	4239094..4239837 [+], 744	407467674	hypothetical protein
37	O3O_04905	4239842..4241257 [+], 1416	407467673	hypothetical protein	TssA
38	O3O_04900	4241276..4244800 [+], 3525	407467672	IcmF-like protein	TssM
39	O3O_04895	4244811..4246163 [+], 1353	407467671	hypothetical protein	TssA
40	O3O_04890	4246187..4246669 [+], 483	407467670	hypothetical protein	TssD
41	O3O_04885	4246713..4247627 [+], 915	407467669	hypothetical protein
42	O3O_04880	4247637..4248116 [+], 480	407467668	hypothetical protein
43	O3O_04875	4248253..4249038 [-], 786	407467667	aminopeptidase
44	O3O_04870	4249574..4250305 [-], 732	407467666	DNA polymerase III subunit epsilon
45	O3O_04865 (rnhA)	4250370..4250837 [+], 468	407467665	ribonuclease H
46	O3O_04860	4250834..4251556 [-], 723	407467664	S-adenosyl-L-methionine-dependent methyltransferase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

ABG57132

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	O3O_04975	4225583..4226101 [-], 519	407467687	hypothetical protein	ABG57132

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Proteins        Genes

N/A

N/A