I. Summary

SecReT6 ID	731
Strain	Serratia marcescens WW4
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_020211
Location	3387021..3410437
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	SMWW4_v1c31390	3383353..3384222 [-], 870	448242899	hypothetical protein
2	SMWW4_v1c31400	3384331..3384906 [-], 576	448242900	hypothetical protein
3	SMWW4_v1c31410	3385613..3385903 [-], 291	448242901	hypothetical protein
4	SMWW4_v1c31420	3386149..3386376 [+], 228	448242902	hypothetical protein
5	SMWW4_v1c31430	3386461..3386574 [-], 114	448242903	hypothetical protein
6	SMWW4_v1c31440	3386642..3386995 [-], 354	448242904	hypothetical protein
7	SMWW4_v1c31450	3387021..3390500 [-], 3480	448242905	type VI secretion protein IcmF	TssM
8	SMWW4_v1c31460	3390536..3392047 [-], 1512	448242906	hypothetical protein	TssL
9	SMWW4_v1c31470	3392044..3393402 [-], 1359	448242907	hypothetical protein	TssK
10	SMWW4_v1c31480	3393404..3393910 [-], 507	448242908	hypothetical protein	TssJ
11	SMWW4_v1c31490	3393920..3394285 [-], 366	448242909	hypothetical protein	PAAR
12	SMWW4_v1c31500	3394292..3394906 [-], 615	448242910	hypothetical protein
13	SMWW4_v1c31510	3394899..3396053 [-], 1155	448242911	hypothetical protein
14	SMWW4_v1c31520	3396043..3398268 [-], 2226	448242912	hypothetical protein
15	SMWW4_v1c31530	3398281..3400542 [-], 2262	448242913	hypothetical protein	TssI
16	SMWW4_v1c31540 (clpV)	3400607..3403192 [-], 2586	448242914	type VI secretion ATPase, ClpV family	TssH
17	SMWW4_v1c31550 (tssG)	3403209..3404237 [-], 1029	448242915	type VI secretion protein, TssG family	TssG
18	SMWW4_v1c31560 (tssF)	3404230..3406071 [-], 1842	448242916	type VI secretion protein, TssF family	TssF
19	SMWW4_v1c31570	3406083..3406529 [-], 447	448242917	hypothetical protein	TssE
20	SMWW4_v1c31580	3406611..3407135 [-], 525	448242918	hypothetical protein	TssD
21	SMWW4_v1c31590	3407367..3408893 [-], 1527	448242919	hypothetical protein	TssC
22	SMWW4_v1c31600 (tssB)	3408890..3409447 [-], 558	448242920	type VI secretion protein, TssB family	TssB
23	SMWW4_v1c31610	3409472..3410437 [-], 966	448242921	ImpA domain protein	TssA
24	SMWW4_v1c31620	3411474..3411665 [+], 192	448242922	hypothetical protein
25	SMWW4_v1c31630	3412047..3412481 [+], 435	448242923	hypothetical protein
26	SMWW4_v1c31640	3412539..3413171 [-], 633	448242924	hypothetical protein
27	SMWW4_v1c31650	3413291..3413761 [-], 471	448242925	hypothetical protein
28	SMWW4_v1c31660	3413751..3415238 [-], 1488	448242926	putative 3-polyprenyl-4-hydroxybenzoate decarboxylase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

YPK_3563 , SMA3455, SMA3456, SMA3980

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	SMWW4_v1c31580	3406611..3407135 [-], 525	448242918	hypothetical protein	YPK_3563
2	SMWW4_v1c41490	4490932..4491414 [+], 483	448243902	type VI secretion system effector	SMA3455
3	SMWW4_v1c41500	4491462..4491944 [+], 483	448243903	type VI secretion system effector	SMA3456
4	SMWW4_v1c46890	5079351..5080346 [-], 996	448244442	hypothetical protein	SMA3980

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Proteins        Genes

Homology Immunity protein list

SMA3979

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	SMWW4_v1c46880	5078773..5079351 [-], 579	448244441	hypothetical protein	SMA3979

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Proteins        Genes

N/A