I. Summary

SecReT6 ID	744
Strain	Pseudomonas denitrificans ATCC 13867
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_020829
Location	3350777..3369689
Comment
Function	N/A
T6SS Type	Type i; subtype i4a
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	H681_14830	3346033..3347355 [-], 1323	472326130	CzcB family heavy metal RND efflux membrane fusion protein
2	H681_14835	3347505..3348485 [-], 981	472326131	CzcC family heavy metal RND efflux outer membrane protein
3	H681_14850	3350049..3350555 [-], 507	472326132	N-acetyltransferase GCN5
4	H681_14855	3350777..3351835 [-], 1059	472326133	hypothetical protein	TssA
5	H681_14860	3351832..3355656 [-], 3825	472326134	type VI secretion protein IcmF	TssM
6	H681_14865	3355653..3356423 [-], 771	472326135	type VI secretion protein DotU	TssL
7	H681_14870	3356436..3357725 [-], 1290	472326136	hypothetical protein	TssK
8	H681_14875	3357821..3358246 [-], 426	472326137	hypothetical protein
9	H681_14880	3358504..3359076 [+], 573	472326138	hypothetical protein	TssB
10	H681_14885	3359101..3360588 [+], 1488	472326139	hypothetical protein	TssC
11	H681_14890	3360662..3361159 [+], 498	472326140	hypothetical protein	TssD
12	H681_14895	3361172..3361600 [+], 429	472326141	hypothetical protein	TssE
13	H681_14900	3361584..3363410 [+], 1827	472326142	type VI secretion protein VasA	TssF
14	H681_14905	3363374..3364387 [+], 1014	472326143	hypothetical protein	TssG
15	H681_14910	3364387..3366936 [+], 2550	472326144	type VI secretion ATPase	TssH
16	H681_14915	3367683..3369689 [+], 2007	472326145	hypothetical protein	TssI
17	H681_14920	3369700..3370239 [+], 540	472326146	hypothetical protein
18	H681_14925	3370303..3371205 [+], 903	472326147	hypothetical protein
19	H681_14930	3371242..3371637 [-], 396	472326148	hypothetical protein
20	H681_14935	3372019..3372714 [-], 696	472326149	hypothetical protein
21	H681_14940	3373084..3374631 [+], 1548	472326150	diguanylate cyclase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PSPTO_5435 , PSPTO_5435

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	H681_06055	1326142..1326660 [-], 519	472324385	hypothetical protein	PSPTO_5435
2	H681_11920	2636050..2636568 [+], 519	472325548	hypothetical protein	PSPTO_5435

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Proteins        Genes

N/A

N/A