I. Summary

SecReT6 ID	747
Strain	Pseudomonas aeruginosa B136-33
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_020912
Location	2892536..2912421
Comment
Function	N/A
T6SS Type	Type i; subtype i4a
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

12

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	G655_13160	2889247..2890452 [-], 1206	478478824	hypothetical protein
2	G655_13165	2890653..2891294 [-], 642	478478825	putative transcriptional regulator
3	G655_13170	2891571..2891966 [+], 396	478478826	hypothetical protein
4	G655_13175	2891989..2892525 [-], 537	478478827	hypothetical protein
5	G655_13180	2892536..2894542 [-], 2007	478478828	hypothetical protein	TssI
6	G655_13185	2894579..2895646 [-], 1068	478478829	hypothetical protein
7	G655_13190	2895685..2896110 [-], 426	478478830	hypothetical protein
8	G655_13195	2896292..2898841 [-], 2550	478478831	putative ClpA/B-type protease	TssH
9	G655_13200	2898843..2899859 [-], 1017	478478832	hypothetical protein	TssG
10	G655_13205	2899823..2901616 [-], 1794	478478833	type VI secretion protein VasA	TssF
11	G655_13210	2901600..2902025 [-], 426	478478834	hypothetical protein	TssE
12	G655_13215	2902038..2902535 [-], 498	478478835	hypothetical protein	TssD
13	G655_13220	2902609..2904093 [-], 1485	478478836	hypothetical protein	TssC
14	G655_13225	2904116..2904661 [-], 546	478478837	hypothetical protein	TssB
15	G655_13230	2904869..2905345 [+], 477	478478838	hypothetical protein
16	G655_13235	2905444..2906736 [+], 1293	478478839	hypothetical protein	TssK
17	G655_13240	2906754..2907512 [+], 759	478478840	type VI secretion protein DotU	TssL
18	G655_13245	2907509..2911324 [+], 3816	478478841	type VI secretion protein IcmF	TssM
19	G655_13250	2911321..2912421 [+], 1101	478478842	hypothetical protein	TssA
20	G655_13255	2912531..2913616 [+], 1086	478478843	putative transcriptional regulator	Sfa3
21	G655_13260	2913852..2914118 [+], 267	478478844	hypothetical protein
22	G655_13265	2914278..2914838 [+], 561	478478845	NADH-dependent FMN reductase MsuE
23	G655_13270	2914848..2915993 [+], 1146	478478846	methanesulfonate sulfonatase MsuD
24	G655_13275	2916023..2917207 [+], 1185	478478847	putative FMNH2-dependent monooxygenase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PA5089

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	G655_26795	5881685..5883922 [-], 2238	478481538	hypothetical protein	PA5089

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Proteins        Genes

Homology Immunity protein list

PA5087, PA5088, PA5087

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	G655_26780	5879050..5879931 [-], 882	478481535	hypothetical protein	PA5087
2	G655_26785	5879928..5880809 [-], 882	478481536	hypothetical protein	PA5088
3	G655_26790	5880806..5881681 [-], 876	478481537	hypothetical protein	PA5087

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Proteins        Genes

N/A