I. Summary
SecReT6 ID75 experimental
StrainBurkholderia pseudomallei K96243
Repliconchromosome II [Browse all T6SS(s) in this replicon]
CommentVirulence in Syrian hamster model; growth and mononuclear giant cell formation in cultured macrophages; intercellular spread in cultured cells
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1BPSS14902031208..2032233 [+], 102653722512N-acetylmuramoyl-L-alanine amidase 
2BPSS14912032502..2033761 [+], 126053722513hypothetical protein 
3BPSS14922033784..2035334 [+], 155153722514hypothetical protein 
4BPSS14932036034..2037980 [-], 194753722515hypothetical protein  TssA
5BPSS14942037977..2038714 [-], 73853722516two-component response regulator protein  VirG
6BPSS14952038711..2040555 [-], 184553722517two-component regulator sensor kinase  VirA
7BPSS14962040815..2041309 [+], 49553722518hypothetical protein  TssB
8BPSS14972041333..2042832 [+], 150053722519hypothetical protein  TssC
9BPSS14982043052..2043561 [+], 51053722520hypothetical protein  TssD
10BPSS14992043554..2044015 [+], 46253722521hypothetical protein  TssE
11BPSS15002044052..2045794 [+], 174353722522hypothetical protein  TssF
12BPSS15012045782..2046804 [+], 102353722523hypothetical protein  TssG
13BPSS15022046791..2049829 [+], 303953722524Clp-type ATPase chaperone protein  TssH
14BPSS15032049856..2052879 [+], 302453722525hypothetical protein  TssI
15BPSS15042052905..2055547 [+], 264353722526hypothetical protein 
16BPSS15052055565..2056629 [+], 106553722527hypothetical protein 
17BPSS15062056626..2057378 [+], 75353722528hypothetical protein 
18BPSS15072057407..2057799 [+], 39353722529hypothetical protein 
19BPSS15082057929..2058606 [+], 67853722530hypothetical protein  TssJ
20BPSS15092058639..2060036 [+], 139853722531hypothetical protein  TssK
21BPSS15102060051..2060698 [+], 64853722532hypothetical protein  TssL
22BPSS15112060710..2064669 [+], 396053722533hypothetical protein  TssM
23BPSS15122065185..2066609 [+], 142553722534hypothetical protein  BMAA0729 (e)
24BPSS15132066773..2067066 [+], 29453722535hypothetical protein 
25BPSS1514 (folE)2067071..2067703 [+], 63353722536GTP cyclohydrolase I 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
BMAA0742 , BMAA0737, BMAA0729

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1BPSS14982043052..2043561 [+], 51053722520hypothetical protein BMAA0742
2BPSS15032049856..2052879 [+], 302453722525hypothetical protein BMAA0737
3BPSS15122065185..2066609 [+], 142553722534hypothetical protein BMAA0729
Data from experimental literature

Download FASTA format files
Proteins        Genes
Regulatory element identified
BsaN (BPSS1546)ActivatorPMID: 21670170

BspR (BPSL1105)Indirect activatorPMID: 21670170

BprC (BPSS1520)ActivatorPMID: 21670170

VirA (BPSS1495)ActivatorPMID: 21670170
VirG (BPSS1494)ActivatorPMID: 21670170

BicA (BPSS1533)ActivatorPMID: 21670170

BprP (BPSS1553)Indirect activatorPMID: 21670170

(1) Chirakul S et al. (2014). Characterization of BPSS1521 (bprD), a Regulator of Burkholderia pseudomallei Virulence Gene Expression in the Mouse Model. PLoS One. 9(8):e104313. [PudMed:25111708] experimental
(2) Chen Y et al. (2014). Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon. BMC Microbiol. 14(1):206. [PudMed:25085508] experimental
(3) Hopf V et al. (2014). BPSS1504, a cluster 1 type VI secretion gene, is involved in intracellular survival and virulence of Burkholderia pseudomallei. Infect Immun. 82(5):2006-15. [PudMed:24595140] experimental
(4) Toesca IJ et al. (2014). The Type VI Secretion System Spike Protein VgrG5 Mediates Membrane Fusion during Intercellular Spread by Pseudomallei Group Burkholderia Species. Infect Immun. 82(4):1436-44. [PudMed:24421040] experimental
(5) Fisher NA et al. (2012). The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 12:117. [PudMed:22892068]
(6) Chen Y et al. (2011). Regulation of type VI secretion system during Burkholderia pseudomallei infection. Infect Immun. 79(8):3064-73. [PudMed:21670170] experimental
(7) Burtnick MN et al. (2011). The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun. 79(4):1512-25. [PudMed:21300775] experimental
(8) Sun GW et al. (2010). Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei. Mol Microbiol. 76(3):677-89. [PudMed:20345664]
(9) Shalom G et al. (2007). In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages. Microbiology. 153(Pt 8):2689-99. [PudMed:17660433] experimental
experimental This literature contains experimental investigation