I. Summary

SecReT6 ID	752
Strain	Enterobacter cloacae subsp. cloacae NCTC 9394
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_021046
Location	935277..962988
Comment
Function	N/A
T6SS Type	Type i; subtype i2
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	0

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	ENC_08860	930787..931752 [+], 966	479271620	L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily
2	ENC_08870	931743..932456 [-], 714	479271621	Predicted carboxypeptidase
3	ENC_08900	935277..935768 [+], 492	479271622	type VI secretion protein, VC_A0107 family	TssB
4	ENC_08910	935803..937344 [+], 1542	479271623	type VI secretion protein, EvpB/VC_A0108 family	TssC
5	ENC_08920	937360..938700 [+], 1341	479271624	type VI secretion protein, VC_A0114 family	TssK
6	ENC_08930	938697..939386 [+], 690	479271625	type IV / VI secretion system protein, DotU family	TssL
7	ENC_08940	939383..941089 [+], 1707	479271626	Outer membrane protein and related peptidoglycan-associated (lipo)proteins	TagL
8	ENC_08950	941095..941586 [+], 492	479271627	type VI secretion system effector, Hcp1 family	TssD
9	ENC_08960	941760..944405 [+], 2646	479271628	type VI secretion ATPase, ClpV1 family	TssH
10	ENC_08970	944402..946894 [+], 2493	479271629	type VI secretion system Vgr family protein	TssI
11	ENC_08980	947632..947796 [+], 165	479271630	hypothetical protein
12	ENC_09000	950888..951283 [+], 396	479271631	hypothetical protein
13	ENC_09010	951313..952044 [+], 732	479271632	Protein of unknown function (DUF3274).
14	ENC_09020	952396..953826 [+], 1431	479271633	hypothetical protein
15	ENC_09050	957564..959165 [+], 1602	479271634	type VI secretion-associated protein, VC_A0119 family	TssA
16	ENC_09060	959207..960961 [+], 1755	479271635	type VI secretion protein, VC_A0110 family	TssF
17	ENC_09070	960925..962010 [+], 1086	479271636	type VI secretion protein, VC_A0111 family	TssG
18	ENC_09090	962533..962988 [+], 456	479271637	type VI secretion system lysozyme-related protein	TssE
19	ENC_09120	964705..965193 [+], 489	479271638	hypothetical protein
20	ENC_09130	965491..965706 [+], 216	479271639	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

EC042_4529 , EC042_4529

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	ENC_08950	941095..941586 [+], 492	479271627	type VI secretion system effector, Hcp1 family	EC042_4529
2	ENC_31190	3152480..3152971 [+], 492	479273322	type VI secretion system effector, Hcp1 family	EC042_4529

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Proteins        Genes

N/A

N/A