I. Summary

SecReT6 ID	760
Strain	Burkholderia thailandensis MSMB121
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_021174
Location	1286631..1308146
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

13

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BTI_4615	1281918..1282298 [-], 381	488606779	hypothetical protein
2	BTI_4616	1282312..1282971 [-], 660	488605865	hypothetical protein
3	BTI_4617	1283043..1284125 [-], 1083	488606730	pentapeptide repeats family protein
4	BTI_4618	1284122..1286599 [-], 2478	488606682	pentapeptide repeats family protein
5	BTI_4619	1286631..1288919 [-], 2289	488606945	Rhs element Vgr family protein	TssI
6	BTI_4620	1288990..1290090 [-], 1101	488606832	impA-related N-terminal family protein	TssA
7	BTI_4621	1290087..1291178 [-], 1092	488607122	hypothetical protein	TssG
8	BTI_4622	1291178..1293067 [-], 1890	488606465	hypothetical protein	TssF
9	BTI_4623	1293096..1293677 [-], 582	488606292	lysozyme family protein	TssE
10	BTI_4624	1293664..1294014 [-], 351	488606370	impE family protein	TagJ
11	BTI_4625	1294011..1294733 [-], 723	488605610	hypothetical protein
12	BTI_4626 (clpV)	1295264..1297975 [+], 2712	488606906	type VI secretion ATPase, ClpV1 family	TssH
13	BTI_4627	1298009..1298590 [+], 582	488607433	hypothetical protein	TssB
14	BTI_4628	1298624..1300123 [+], 1500	488606934	hypothetical protein	TssC
15	BTI_4629 (hcp1)	1300240..1300725 [+], 486	488607525	protein hcp1	TssD
16	BTI_4630	1300821..1301324 [+], 504	488605432	type VI secretion lipofamily protein	TssJ
17	BTI_4631	1301346..1302692 [+], 1347	488606499	hypothetical protein	TssK
18	BTI_4632	1302781..1304055 [+], 1275	488607242	type VI secretion system OmpA/MotB family protein	TssL
19	BTI_4633	1304085..1308146 [+], 4062	488605363	intracellular multiplication and macrophage-killing family protein	TssM
20	BTI_4634	1308257..1308553 [-], 297	488605587	hypothetical protein
21	BTI_4635	1308927..1310843 [-], 1917	488606626	SMP-30/Gluconolaconase/LRE-like region family protein
22	BTI_4636	1311460..1312221 [+], 762	488607024	short chain dehydrogenase family protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

SciK

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BTI_4629(hcp1)	1300240..1300725 [+], 486	488607525	protein hcp1	SciK

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Proteins        Genes

N/A

N/A