I. Summary

SecReT6 ID	765
Strain	Burkholderia sp. RPE64
Replicon	chromosome I [Browse all T6SS(s) in this replicon]
Accession	NC_021287
Location	337535..346771
Comment
Function	N/A
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	0	1	0	0	1	0	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	0	0

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BRPE64_ACDS03290 (rplM)	332662..333090 [+], 429	507514862	50S ribosomal protein L13
2	BRPE64_ACDS03300 (rpsI)	333102..333494 [+], 393	507514863	30S ribosomal protein S9
3	BRPE64_ACDS03310	333737..334069 [+], 333	507514864	putative iron-sulfur cluster insertion protein ErpA 1
4	BRPE64_ACDS03320	334158..335327 [-], 1170	507514865	anhydro-N-acetylmuramic acid kinase
5	BRPE64_ACDS03330 (tyrS)	335648..336904 [+], 1257	507514866	tyrosine--tRNA ligase
6	BRPE64_ACDS03340 (dtd)	336909..337367 [+], 459	507514867	D-tyrosyl-tRNA(Tyr) deacylase
7	BRPE64_ACDS03350	337535..339031 [+], 1497	507514868	hypothetical protein	TssC
8	BRPE64_ACDS03360	339157..339642 [+], 486	507514869	type VI secretion system effector Hcp1 family	TssD
9	BRPE64_ACDS03370	339754..340269 [+], 516	507514870	hypothetical protein	TssJ
10	BRPE64_ACDS03380	340640..341119 [+], 480	507514871	hypothetical protein	TssE
11	BRPE64_ACDS03390	342635..344527 [+], 1893	507514872	hypothetical protein	TssF
12	BRPE64_ACDS03400	344530..345588 [+], 1059	507514873	type VI secretion protein VC_A0111 family	TssG
13	BRPE64_ACDS03410	345677..346771 [+], 1095	507514874	ImpA domain protein	TssA
14	BRPE64_ACDS03420 (ruvB)	346795..347853 [-], 1059	507514875	Holliday junction ATP-dependent DNA helicase RuvB
15	BRPE64_ACDS03430 (ruvA)	347911..348492 [-], 582	507514876	Holliday junction ATP-dependent DNA helicase RuvA
16	BRPE64_ACDS03440 (ruvC)	348517..349059 [-], 543	507514877	crossover junction endodeoxyribonuclease RuvC
17	BRPE64_ACDS03450 (purH)	349176..350750 [-], 1575	507514878	bifunctional purine biosynthesis protein PurH
18	BRPE64_ACDS03460 (fis)	350806..351039 [-], 234	507514879	DNA-binding protein Fis

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

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