I. Summary

SecReT6 ID	77
Strain	Burkholderia pseudomallei K96243
Replicon	chromosome I [Browse all T6SS(s) in this replicon]
Accession	NC_006350
Location	3697190..3717176
Comment
Function	N/A
T6SS Type	Type i; subtype i4b
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	0	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BPSL3094	3693290..3694738 [+], 1449	53720703	outer membrane bacteriocin efflux protein
2	BPSL3095	3694948..3695934 [-], 987	53720704	hypothetical protein
3	BPSL3096	3696333..3696911 [+], 579	53720705	bacteriophage-related peptidase
4	BPSL3097	3697190..3701083 [+], 3894	53720706	hypothetical protein	TssM
5	BPSL3098	3701080..3702069 [+], 990	53720707	hypothetical protein
6	BPSL3099	3702074..3703006 [+], 933	53720708	outer membrane protein	TagL
7	BPSL3100	3703205..3704326 [-], 1122	53720709	hypothetical protein	TssA
8	BPSL3101 (clpB)	3704416..3707085 [-], 2670	53720710	protease associated ATPase ClpB	TssH
9	BPSL3102	3707119..3708219 [-], 1101	53720711	hypothetical protein	TssG
10	BPSL3103	3708183..3710009 [-], 1827	53720712	hypothetical protein	TssF
11	BPSL3104	3710101..3710613 [-], 513	53720713	hypothetical protein	TssE
12	BPSL3105	3710641..3711144 [-], 504	53720714	hypothetical protein	TssD
13	BPSL3106	3711217..3712707 [-], 1491	53720715	hypothetical protein	TssC
14	BPSL3107	3712724..3713242 [-], 519	53720716	hypothetical protein	TssB
15	BPSL3108	3713279..3713950 [-], 672	53720717	lipoprotein
16	BPSL3109	3714325..3714939 [+], 615	53720718	lipoprotein	TssJ
17	BPSL3110	3715048..3716394 [+], 1347	53720719	hypothetical protein	TssK
18	BPSL3111	3716391..3717176 [+], 786	53720720	hypothetical protein	TssL
19	BPSL3114	3718434..3719234 [+], 801	53720721	hypothetical protein
20	BPSL3115	3719241..3720311 [+], 1071	53720722	transcriptional regulator
21	BPSL3116	3720594..3720857 [+], 264	53720723	transposase
22	BPSL3117	3720854..3721714 [+], 861	53720724	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

BTH_I0068 , BTH_I2693, BTH_I2697, BTH_I2698, BTH_I2705, BCAL0343, BTH_I3225, BTH_II0090, BTH_I3226, BTH_I3225, BTH_II0310

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BPSL0067	76057..76497 [+], 441	53717706	hypothetical protein	BTH_I0068
2	BPSL2041	2438558..2441398 [-], 2841	53719652	hypothetical protein	BTH_I2693
3	BPSL2046	2443535..2446870 [-], 3336	53719658	hypothetical protein	BTH_I2697
4	BPSL2047	2446979..2449387 [-], 2409	53719659	hypothetical protein	BTH_I2698
5	BPSL2050	2451504..2454302 [-], 2799	53719663	hypothetical protein	BTH_I2705
6	BPSL3105	3710641..3711144 [-], 504	53720714	hypothetical protein	BCAL0343
7	BPSS0078	88921..91692 [+], 2772	53721116	hypothetical protein	BTH_I3225
8	BPSS0079	91706..93946 [+], 2241	53721117	hypothetical protein	BTH_II0090
9	BPSS1212	1633710..1635902 [-], 2193	53722235	hypothetical protein	BTH_I3226
10	BPSS1213	1635964..1638741 [-], 2778	53722236	hypothetical protein	BTH_I3225
11	BPSS2022	2732831..2733736 [+], 906	53723040	outer membrane protein	BTH_II0310

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Proteins        Genes

Homology Immunity protein list

BTH_I0069, BTH_I2700

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BPSL0068	76484..76852 [+], 369	53717707	lipoprotein	BTH_I0069
2	BPSL2048A	2449974..2450531 [-], 558	53719661	hypothetical protein	BTH_I2700

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Proteins        Genes

(1) Burtnick MN et al. (2011). The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun. 79(4):1512-25. [PudMed:21300775]

This literature contains experimental investigation