I. Summary

SecReT6 ID	784
Strain	Ralstonia solanacearum CMR15
Replicon	plasmid CMR15_mp [Browse all T6SS(s) in this replicon]
Accession	NC_017589
Location	820709..861362
Comment
Function	N/A
T6SS Type	Type i; subtype i4b
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	3	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

15

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	CMR15_mp10703	815883..816941 [-], 1059	410684611	conserved hypothethical protein
2	CMR15_mp10704	816973..818031 [-], 1059	410684612	conserved hypothethical protein
3	CMR15_mp10705	818057..819127 [-], 1071	410684613	conserved hypothethical protein
4	CMR15_mp10706	819139..820689 [-], 1551	410684614	conserved hypothethical protein
5	CMR15_mp10707	820709..823510 [-], 2802	410684615	conserved hypothethical protein, Rhs element Vgr related protein	TssI
6	CMR15_mp10708	823569..824360 [-], 792	410684616	putative secretion system protein (EvpN-like)	TssL
7	CMR15_mp10709	824357..825703 [-], 1347	410684617	conserved hypothethical protein	TssK
8	CMR15_mp10710	825747..826421 [-], 675	410684618	conserved exported protein of unknown function	TssJ
9	CMR15_mp10711	826408..826578 [-], 171	410684619	hypothetical protein
10	CMR15_mp10712	826696..827343 [+], 648	410684620	conserved exported protein of unknown function, partial TRP motif
11	CMR15_mp10713	827380..827892 [+], 513	410684621	conserved hypothethical protein DUF770	TssB
12	CMR15_mp10714	827873..829375 [+], 1503	410684622	conserved hypothethical protein	TssC
13	CMR15_mp10715 (sciK)	829463..829966 [+], 504	410684623	secretion system protein (EvpC-like), Hemolysin-coregulated protein	TssD
14	CMR15_mp10716	830027..830500 [+], 474	410684624	putative secretion system protein (EvpE-like)	TssE
15	CMR15_mp10717	830563..832413 [+], 1851	410684625	putative Type VI secretion protein (DUF879)	TssF
16	CMR15_mp10718	832377..833468 [+], 1092	410684626	putative Type VI secretion protein, VC_A0111 family	TssG
17	CMR15_mp10719 (clpB)	833501..836218 [+], 2718	410684627	Chaperone clpB	TssH
18	CMR15_mp10720	836239..838995 [+], 2757	410684628	putative Rhs element VGR-related protein	TssI
19	CMR15_mp10721	838992..840146 [+], 1155	410684629	conserved hypothethical protein
20	CMR15_mp10722	840124..842877 [+], 2754	410684630	conserved hypothethical protein
21	CMR15_mp10723	842861..843994 [+], 1134	410684631	conserved hypothethical protein
22	CMR15_mp10724	844089..844208 [+], 120	410684632	conserved hypothethical protein
23	CMR15_mp10725	844391..844798 [+], 408	410684633	conserved exported protein of unknown function
24	CMR15_mp10726	845053..846405 [+], 1353	410684634	putative lipoprotein yddW precursor
25	CMR15_mp10727	846537..847616 [+], 1080	410684635	conserved hypothethical protein	TssA
26	CMR15_mp10728	847686..848651 [-], 966	410684636	putative transmembrane protein
27	CMR15_mp10729	848750..849589 [-], 840	410684637	putative outer membrane protein; OmpA/MotB domain	TagL
28	CMR15_mp10730	849586..850572 [-], 987	410684638	conserved hypothethical protein (signal transduction histidine kinase domain)
29	CMR15_mp10731	850569..854480 [-], 3912	410684639	putative secretion system protein (EvpO-like)	TssM
30	CMR15_mp10732	854518..855975 [-], 1458	410684640	conserved transmembrane protein of unknown function
31	CMR15_mp10733	856016..856285 [-], 270	410684641	conserved hypothethical protein with PAAR_motif	PAAR
32	CMR15_mp10734	856325..857173 [-], 849	410684642	putative carboxypeptidase
33	CMR15_mp10735	857459..858205 [+], 747	410684643	putative transmembrane protein
34	CMR15_mp10736	858342..861362 [+], 3021	410684644	conserved hypothethical protein, VGR related protein	TssI
35	CMR15_mp10737	861378..862784 [+], 1407	410684645	putative hydrolase
36	CMR15_mp10738	862856..863950 [-], 1095	410684646	putative transposase
37	CMR15_mp10740	865390..865671 [+], 282	410684647	conserved exported protein of unknown function
38	CMR15_mp10741	865803..866267 [+], 465	410684648	membrane protein of unknown function

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

RSp0745

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	CMR15_mp10715(sciK)	829463..829966 [+], 504	410684623	secretion system protein (EvpC-like), Hemolysin-coregulated protein	RSp0745

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Proteins        Genes

N/A

N/A