SecReT6 ID	788
Strain	Aeromonas hydrophila SSU
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	DQ667172
Location	1..27577
Comment	Cytotoxicity and inhibition of phagocytosis in cultured macrophages and epithelial cells; full virulence in septicaemic mouse model
Function	anti-eukaryotic; effector translocation;virulence
T6SS Type	Type i; subtype i1
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	2	0	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	- (hcp2)	1..519 [+], 519	110277123	Hcp-2 hemolysin-coregulated protein	TssD
2	- (vgrG2)	1004..3049 [+], 2046	110277124	VgrG-2 protein	TssI
3	-	3049..3918 [+], 870	110277125	hypothetical protein
4	-	3915..4559 [+], 645	110277126	hypothetical protein
5	-	4660..5631 [+], 972	110277127	hypothetical protein	TssC
6	-	5638..6069 [+], 432	110277128	hypothetical protein	TssE
7	- (vasA)	6073..7839 [+], 1767	110277129	VasA	TssF
8	-	7803..8801 [+], 999	110277130	hypothetical protein	TssG
9	-	8858..10138 [+], 1281	110277131	hypothetical protein	Fha
10	-	10138..10653 [+], 516	110277132	hypothetical protein	TssJ
11	-	10656..11990 [+], 1335	110277133	hypothetical protein	TssK
12	- (vasF)	12082..12792 [+], 711	110277134	VasF	TssL
13	- (clpB)	12813..15455 [+], 2643	110277135	ClpB protein	TssH
14	- (vasH)	15458..16996 [+], 1539	110277136	VasH
15	-	16996..17601 [+], 606	110277137	hypothetical protein
16	-	17610..19055 [+], 1446	110277138	hypothetical protein	TssA
17	- (vasK)	19097..22582 [+], 3486	110277139	VasK	TssM
18	-	22629..24059 [+], 1431	110277140	hypothetical protein	TssA
19	-	24317..24604 [+], 288	110277141	hypothetical protein	PAAR
20	- (vgrG3)	24615..26663 [+], 2049	110277142	VgrG-3 protein	TssI
21	-	26704..27577 [+], 874	110277143	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files

Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

VgrG2 (ABG57133)   VgrG3 (ABG57151)   Hcp2 (ABG57132)   Hcp1 (AFX59894)   VgrG1 (AFX59895)   TesC(ORF2402)

Homology effector (effector protein label, when available)

Homology immunity protein (immunity protein label, when available)

Regulatory element identified

AhyR

Regulator	Description	Reference
AhyI	Activator	PMID: 19729404
AhyR	Activator	PMID: 19729404

VasH

Regulator	Description	Reference
VasH	Activator	PMID: 18037263

Adaptors list

ORF2403

Homology effector (effector protein label, when available)

(1) Grim CJ et al. (2014). Functional Genomic Characterization of Virulence Factors from Necrotizing Fasciitis-Causing Strains of Aeromonas hydrophila. Appl Environ Microbiol. 80(14):4162-83. [PudMed:24795370]

(2) Sha J et al. (2013). Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology. 159(Pt 6):1120-35. [PudMed:23519162]

(3) Suarez G et al. (2010). Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology. 156(Pt 12):3678-88. [PudMed:20798163]

(4) Suarez G et al. (2010). A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 192(1):155-68. [PudMed:19880608]

(5) Suarez G et al. (2008). Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 44(4):344-61. [PudMed:18037263]

This literature contains experimental investigation