SecReT6
SecReT6 ID790 experimental
StrainVibrio cholerae V52
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNZ_AAKJ02000013
Location12546..38192
CommentCytotoxicity towards Dictyostelium and cultured mammalian macrophages; inflammatory response, diarrhoea and actin cross-linking in mouse intestine; Killing of E. coli, Salmonella and Citrobacter rodentium
Functionanti-bacterial and anti-eukaryotic; effector translocation;virulence; adherence
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number101121111
ComponentTssGTssJTssKTssM
Number1111
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1VCV52_A0145 (rbsB)7969..8853 [-], 885487815976D-ribose transporter subunit RbsB 
2VCV52_A01448908..9891 [-], 984446029754ribose ABC transporter permease 
3VCV52_A0143 (rbsA)9906..11408 [-], 1503446111600D-ribose transporter ATP binding protein 
4VCV52_A0142 (rbsD)11435..11905 [-], 471487815988D-ribose pyranase 
5VCV52_A014112138..12398 [+], 261446721925hypothetical protein 
6VCV52_A014012546..12914 [-], 369446984039hypothetical protein  TsiV3 (i)
7VCV52_A0139 (vgrG-3)12911..15964 [-], 3054446035450type VI secretion protein VgrG  TssI
8VCV52_A013816203..17468 [-], 1266487815979type VI secretion protein  TssA
9VCV52_A013717516..21061 [-], 3546487815985type VI secretion protein IcmF  TssM
10VCV52_A013621077..22486 [-], 1410446348238type VI secretion protein  TssA
11VCV52_A013522496..23179 [-], 684487815980type VI secretion protein 
12VCV52_A013423149..24741 [-], 1593446006947ATPase AAA  VasH
13VCV52_A0133 (clpB-2)24744..27353 [-], 2610446541787ATPase AAA  TssH
14VCV52_A013227379..28152 [-], 774446005552hypothetical protein  TssL
15VCV52_A013128235..29491 [-], 1257487815977hypothetical protein  TssK
16VCV52_A013029498..29974 [-], 477446968055type VI secretion protein  TssJ
17VCV52_A012929977..31464 [-], 1488447012450signal peptide protein  Fha
18VCV52_A012831467..32420 [-], 954487815978hypothetical protein  TssG
19VCV52_A012732447..34216 [-], 1770446111937type VI secretion protein  TssF
20VCV52_A012634222..34659 [-], 438446143146lysozyme  TssE
21VCV52_A012534662..36140 [-], 1479487809500type VI secretion protein  TssC
22VCV52_A012436181..36687 [-], 507445953536type VI secretion protein  TssB
23VCV52_A012337119..38192 [-], 1074487815982hypothetical protein 
24VCV52_A0122 (rluA-2)40177..40842 [+], 666487814261ribosomal large subunit pseudouridine synthase A 
25VCV52_A012140884..42662 [-], 1779446929257sulfate permease 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
VCA0123 (VCV52_A0139) , VCA0118 (VCV52_A0135)   VC1416 (VCV52_1390)   VCA0018 (VCV52_A0041)   VC1418 (VCV52_1392)   VCA0020 (VCV52_A0043)   VC1415 (VCV52_1389)   VCA0285(VCV52_A0301)   VCA0017 (VCV52_A0040)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1VCV52_A0139(vgrG-3)12911..15964 [-], 3054446035450type VI secretion protein VgrG (VCV52_A0139)VCA0123 (VCV52_A0139)

Download FASTA format files
Proteins        Genes

Homology Immunity protein list
   VCA0021 (VCV52_A0044)   VC1419 (VCV52_1393)   VCA0124 (VCV52_A0140)   VCA0286(VCV52_A0302)
Homology immunity protein (immunity protein label, when available)
Regulatory element identified
VasH
RegulatorDescriptionReference
VasH (VCV52_A0134)ActivatorPMID: 21949076

HapR
RegulatorDescriptionReference
HapRActivatorPMID: 19701456

LuxQ
RegulatorDescriptionReference
LuxQ (VCV52_A0685)Can be activator or repressorPMID: 19701456

OscR
RegulatorDescriptionReference
OscR (VCV52_A0051)RepressorPMID: 22083711

VCA0122
RegulatorDescriptionReference
VCA0122ActivatorPMID: 21909372

HlyU
RegulatorDescriptionReference
HlyUActivatorPMID: 8557353

LuxS
RegulatorDescriptionReference
LuxS (VCV52_0530)ActivatorPMID: 19701456

Hfq
RegulatorDescriptionReference
HfqRepressorPMID: 19701456

DksA
RegulatorDescriptionReference
DksA (VCV52_0564)ActivatorPMID: 19329638

LuxO
RegulatorDescriptionReference
LuxO (VCV52_0978)Repressor: HapRPMID: 21084635

TsrA
RegulatorDescriptionReference
TsrA (VCV52_0073)RepressorPMID: 21084635

Qrr sRNAs
RegulatorDescriptionReference
Qrr sRNAsRepressorPMID: 24698180

RpoN
RegulatorDescriptionReference
RpoN (VCV52_2488)Activator: Hcp operons and VgrG-3PMID: 22723378

TfoX
RegulatorDescriptionReference
TfoXActivatorPMID: 25554784

QstR
RegulatorDescriptionReference
QstRActivatorPMID: 25554784

thiourea
RegulatorDescriptionReference
thioureaRepressorPMID: 25646446


Adaptors list
Tap-1
Homology effector (effector protein label, when available)
(1) Borgeaud S et al. (2015). Bacterial evolution. The type VI secretion system of Vibrio cholerae fosters horizontal gene transfer. Science. 347(6217):63-7. [PudMed:25554784] experimental
(2) Shao Y et al. (2014). Quorum regulatory small RNAs repress type VI secretion in Vibrio cholerae. Mol Microbiol. 92(5):921-30. [PudMed:24698180] experimental
(3) Unterweger D et al. (2014). The Vibrio cholerae type VI secretion system employs diverse effector modules for intraspecific competition. Nat Commun. 5:3549. [PudMed:24686479] experimental
(4) Dong TG et al. (2013). Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc Natl Acad Sci U S A . 110(7):2623-8. [PudMed:23362380] experimental
(5) Miyata ST et al. (2011). Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun. 79(7):2941-9. [PudMed:21555399] experimental
(6) Ma AT et al. (2010). In vivo actin cross-linking induced by Vibrio cholerae type VI secretion system is associated with intestinal inflammation. Proc Natl Acad Sci U S A. 107(9):4365-70. [PudMed:20150509]
(7) Ma AT et al. (2009). Translocation of a Vibrio cholerae type VI secretion effector requires bacterial endocytosis by host cells. Cell Host Microbe. 5(3):234-43. [PudMed:19286133] experimental
(8) Pukatzki S et al. (2006). Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci U S A. 103(5):1528-33. [PudMed:16432199] experimental
 
experimental This literature contains experimental investigation

Genomics & Bioinformatics Group in MML, SJTU